{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:26:17Z","timestamp":1791044777309,"version":"4.1.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T00:00:00Z","timestamp":1645142400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate whether trophectoderm (TE) biopsy differentially influence the level of serum \u03b2-human chorionic gonadotropin (\u03b2-hCG) with different TE-scored blastocysts transferred in early pregnancy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This retrospective cohort study contained 7847 single-blastocyst transfer cycles executed between January 2019 and June 2020, including 2657 preimplantation genetic testing (PGT) cycles and 5190 <jats:italic>in vitro<\/jats:italic> fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles. All cycles were classified into biopsy and control groups, and further stratified based on the TE morphological scores into three subgroups: grades A, B, and C for TE scores, respectively. Intra-group and inter-group analyses were performed on serum \u03b2-hCG levels on the 12th day after blastocyst transfer (HCG<jats:sub>12<\/jats:sub>), and obstetric and neonatal outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For cycles with a live birth, in grade A TE score subgroups, the HCG<jats:sub>12<\/jats:sub> level did not exhibit statistical significance between the control and biopsy groups after adjustment (769 mIU\/mL vs. 753 mIU\/mL, P=0.631). In contrast, in grade B and C TE score subgroups, the control group showed a significantly higher level of HCG<jats:sub>12<\/jats:sub> relative to the biopsy group (690 mIU\/mL vs. 649 mIU\/mL, P=0.001; 586 mIU\/mL vs. 509 mIU\/mL, P&amp;lt;0.001, respectively). We observed no statistically significant differences in obvious adverse obstetric and neonatal outcomes between the same TE-score subgroups of the biopsy group and control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>While blastocysts with higher TE grades produced higher serum \u03b2-hCG levels in early pregnancy, TE biopsy might exert a negative impact on serum \u03b2-hCG levels by blastocysts with a grade-B TE score and below. TE biopsy did not increase the risk for adverse obstetric and neonatal outcomes.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2022.794720","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T06:37:21Z","timestamp":1645166241000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Trophectoderm Biopsy Differentially Influences the Level of Serum \u03b2-Human Chorionic Gonadotropin With Different Embryonic Trophectoderm Scores in Early Pregnancy From 7847 Single-Blastocyst Transfer Cycles"],"prefix":"10.3389","volume":"13","author":[{"given":"Yuan","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingnan","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuoping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keli","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Xiu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,2,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-016-0667-7","article-title":"Molecular Analysis of DNA in Blastocoele Fluid Using Next-Generation Sequencing","volume":"33","author":"Zhang","year":"2016","journal-title":"J Assist Reprod Genet"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.g7611","article-title":"Clinical Applications of Preimplantation Genetic Testing","volume":"350","author":"Brezina","year":"2015","journal-title":"BMJ"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoaa020","article-title":"ESHRE PGT Consortium and SIG Embryology Good Practice Recommendations for Polar Body and Embryo Biopsy for PGT","volume":"2020","author":"Kokkali","year":"2020","journal-title":"Hum Reprod Open"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmaa019","article-title":"The Dawn of the Future: 30 Years From the First Biopsy of a Human Embryo. 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