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Endocrinol."],"abstract":"<jats:p>Obesity is a growing medical and social problem worldwide. The control of energy homeostasis in the brain is achieved by various regions including the arcuate hypothalamic nucleus (ARH). The latter comprises a number of neuronal populations including the first order metabolic neurons, appetite-stimulating agouti-related peptide (AgRP) neurons and appetite-suppressing proopiomelanocortin (POMC) neurons. Using an <jats:italic>in vivo<\/jats:italic> reductionist approach and POMC<jats:sup>Cre<\/jats:sup>-dependent CRISPR-Cas9, we demonstrate that miR-15a-5p protects from obesity. Moreover, we have identified <jats:italic>Bace1<\/jats:italic>, a gene previously linked to energy metabolism imbalance, as a direct target of miR-15a-5p. This work warrants further investigations of non-coding RNA-mediated regulation of energy homeostasis and might contribute to the development of novel therapeutic approaches to treat metabolic diseases.<\/jats:p>","DOI":"10.3389\/fendo.2022.867929","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T14:55:53Z","timestamp":1657205753000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["In Vivo Reductionist Approach Identifies miR-15a Protecting Mice From Obesity"],"prefix":"10.3389","volume":"13","author":[{"given":"Nicola","family":"Murgia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syeda 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