{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:25:36Z","timestamp":1788492336132,"version":"build-2803163510"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:p>Postmenopausal Osteoporosis (PMOP) is the most prevalent primary osteoporosis, attributable to an imbalance in osteoblast and osteoclast activity. Modified You-Gui-Yin (MYGY), a traditional Chinese herbal formula, is able to effectively treat PMOP, while the critical components and pharmacological mechanisms of MYGY are still unclear. In this study, we aimed to investigate the therapeutic effects and underlying mechanisms of N-butanol extract of MYGY (MYGY-Nb) in ovariectomized (OVX)-induced osteoporosis mice. Histological staining and micro-computed tomography (\u03bcCT) analysis showed that MYGY-Nb was more effective in the suppression of OVX-induced bone loss than MYGY original formula. Subsequently, liquid chromatography and mass spectrometry analysis identified 16 critical compounds of MYGY-Nb and some of them are reported to affect osteoclast functions. Furthermore, <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> experiments demonstrated that MYGY-Nb significantly attenuated osteoclastogenesis by down-regulating RANKL-mediated NF-\u03baB signaling. In conclusion, our study indicated that MYGY-Nb suppresses NF-\u03baB signaling and osteoclast formation to mitigate bone loss in PMOP, implying that MYGY-Nb and its compounds are potential candidates for development of anti-PMOP drugs.<\/jats:p>","DOI":"10.3389\/fendo.2022.925848","type":"journal-article","created":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T04:43:12Z","timestamp":1656045792000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["N-Butanol Extract of Modified You-Gui-Yin Attenuates Osteoclastogenesis and Ameliorates Osteoporosis by Inhibiting RANKL-Mediated NF-\u03baB Signaling"],"prefix":"10.3389","volume":"13","author":[{"given":"Qinghe","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houfu","family":"Ling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mancang","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taotao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pinger","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongfeng","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongting","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hangbo","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fusheng","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00113-015-0085-9","article-title":"[Regulation of Bone Metabolism in Osteoporosis: Novel Drugs for Osteoporosis in Development]","volume":"118","author":"Jakob","year":"2015","journal-title":"Unfallchirurg"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.061113","article-title":"Incidence and Economic Burden of Osteoporosis-Related Fractures in the United State","volume":"22","author":"Burge","year":"2007","journal-title":"J Bone Miner Res"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.7861\/clinmedicine.14-2-187","article-title":"Osteoporosis and Fracture Risk in Older People","volume":"14","author":"Coughlan","year":"2014","journal-title":"Clin Med (Lond)"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.04.010","article-title":"Pharmacological Inhibition of Cathepsin K: A Promising Novel Approach for Postmenopausal Osteoporosis Therapy","volume":"117","author":"Mukherjee","year":"2016","journal-title":"Biochem Pharmacol"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62349-5","article-title":"Osteoporosis: Now and the Future","volume":"377","author":"Rachner","year":"2011","journal-title":"Lancet"},{"key":"B6","doi-asserted-by":"publisher","first-page":"898","DOI":"10.1016\/S2213-8587(17)30188-2","article-title":"Osteoporosis Treatment: Recent Developments and Ongoing Challenges","volume":"5","author":"Khosla","year":"2017","journal-title":"Lancet Diabetes Endocrinol"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.11032","article-title":"Osteoporosis Therapy in Postmenopausal Women With High Risk of Fracture","volume":"316","author":"Cappola","year":"2016","journal-title":"JAMA"},{"key":"B8","first-page":"1","article-title":"Extraction, Isolation and Characterization of Bioactive Compounds From Plants' Extracts","volume":"8","author":"Sasidharan","year":"2011","journal-title":"Afr J Tradit Complement Altern Med"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.632698","article-title":"Pretreatments for the Efficient Extraction of Bioactive Compounds From Plant-Based Biomaterials","volume":"54","author":"Zhao","year":"2014","journal-title":"Crit Rev Food Sci Nutr"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1586\/17512433.2016.1171712","article-title":"Herbal Medicines: Challenges in the Modern World. 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