{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:43:56Z","timestamp":1779914636234,"version":"3.53.1"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T00:00:00Z","timestamp":1657756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate the effect of elevated sperm DNA fragmentation index (DFI) on fresh and frozen embryo transfer cycles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A retrospective study was performed with 549 fresh embryo transfer cycles and 1340 frozen embryo transfer cycles after <jats:italic>in vitro<\/jats:italic> fertilization\/intracytoplasmic sperm injection (IVF\/ICSI) from 2016 to 2021.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The statistical results of 549 fresh embryo transfer cycles showed that the delivery rate in the normal sperm DFI group (43.9% vs. 27.1%, <jats:italic>P<\/jats:italic> = 0.014) was significantly higher than that in the abnormal sperm DFI group, and there were no significant differences in the biochemical pregnancy rate (59.0% vs. 50.8%, <jats:italic>P<\/jats:italic> = 0.232), clinical pregnancy rate (53.1% vs. 40.7%, <jats:italic>P<\/jats:italic> = 0.072), or miscarriage rate (17.3% vs. 33.3%, <jats:italic>P<\/jats:italic> = 0.098) between the two groups. The results of 1340 frozen embryo transfer cycles showed that the biochemical pregnancy rate (57.9% vs. 45.6%, <jats:italic>P<\/jats:italic> = 0.006) and clinical pregnancy rate (50.3% vs. 40.7%, <jats:italic>P<\/jats:italic> = 0.027) in the normal sperm DFI group were significantly higher than those in the abnormal sperm DFI group. The delivery rate (40.9% vs. 33.3%, <jats:italic>P<\/jats:italic> = 0.074) and miscarriage rate (18.6% vs. 18.0%, <jats:italic>P<\/jats:italic> = 0.919) were not significantly different between the two groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The increase of sperm DFI significantly reduced the delivery rate of fresh embryo transfer cycles and the biochemical pregnancy rate and clinical pregnancy rate of frozen embryo transfer cycles.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2022.945242","type":"journal-article","created":{"date-parts":[[2022,7,14]],"date-time":"2022-07-14T05:48:25Z","timestamp":1657777705000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":21,"title":["Influence of sperm DNA fragmentation on the clinical outcome of in vitro fertilization-embryo transfer 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