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Endocrinol."],"abstract":"<jats:p>Circular RNAs (circRNAs) are non-coding RNAs newly identified and play important roles in RNA regulation. However, little is known regarding photoperiods induced circRNAs in the thyroid gland. In this study, we performed a comprehensive analysis of circRNA profiles in the thyroid gland of OVX+E<jats:sub>2<\/jats:sub> ewes at different photoperiods by whole transcriptome sequencing. A total of 37,470 novel circRNAs were detected in different photoperiods (42 days of short photoperiod treatment, SP42; 42 days of long photoperiod treatment, LP42; SP42 transfer to LP42, SPLP42), with a total of 817 circRNAs for SP42-LP42 (down: 132; up: 114), LP42-SPLP42 (down: 136; up: 112) and SP42-SPLP42 (down: 182; up: 141) having differentially expressed. Functional enrichment annotation analysis of DE-circRNAs for GO and KEGG by R package, features that influence photoperiod response in Sunite ewes through the Inositol phosphate metabolism, cGMP-PKG signaling pathway, Calcium signaling pathway, MAPK signaling pathway, and Oocyte meiosis. In addition, competitive endogenous RNA (ceRNA) network analysis revealed target binding sites for identified miRNAs in DE-cirRNAs such as oar-miR-10b, oar-miR-200c, oar-miR-21, oar-miR-370-3p, oar-miR-377-3p, oar-miR-181a, oar-miR-432, and oar-miR-495-3p. These results of this study will provide some new information for understanding circRNA function as well as the changes in the sheep thyroid gland under different photoperiods.<\/jats:p>","DOI":"10.3389\/fendo.2022.974518","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T05:05:43Z","timestamp":1661749543000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes"],"prefix":"10.3389","volume":"13","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyun","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Di","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingxing","family":"Chu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00512","article-title":"Expression and functional analysis of the BCL2-associated agonist of cell death (BAD) gene in the sheep ovary during the reproductive cycle","volume":"9","author":"Cao","year":"2018","journal-title":"Front Endocrinol (Lausanne)"},{"key":"B2","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1038\/s41574-019-0237-z","article-title":"Interconnection between circadian clocks and thyroid function","volume":"15","author":"Ikegami","year":"2019","journal-title":"Nat Rev Endocrinol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202000961R","article-title":"Thyroid hormone receptors are required for the melatonin-dependent control of rfrp gene expression in mice","volume":"34","author":"Quignon","year":"2020","journal-title":"FASEB J"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1242\/dev.145615","article-title":"Development of the thyroid gland","volume":"144","author":"Nilsson","year":"2017","journal-title":"Development"},{"key":"B5","article-title":"\"Ontogeny, anatomy, metabolism and physiology of the thyroid,\"","volume-title":"Endotext","author":"Maenhaut","year":"2000"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1080\/10408363.2016.1269309","article-title":"Thyroid in pregnancy: from physiology to screening","volume":"54","author":"Springer","year":"2017","journal-title":"Crit Rev Clin Lab Sci"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-3-1635","article-title":"Role of the thyroid gland in seasonal reproduction. 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