{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:13:16Z","timestamp":1787026396357,"version":"build-2736575974"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The diverse subtypes of thyroid carcinoma have distinct clinical outcomes despite a comparable spectrum of underlying genetic alterations. Beyond genetic alterations, sparse efforts have been made to characterize the microbes associated with thyroid cancer. In this study, we examine the microbial profile of thyroid cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We sequenced the whole transcriptome of 70 thyroid cancers (40 papillary and 30 anaplastic). Using Infectious Pathogen Detector IPD 2.0, we analysed the relative abundance of 1060 microbes across 70 tumours from patients with thyroid cancer against 118 tumour samples from patients with breast, cervical, colorectal, and tongue cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our analysis reveals a significant prevalence of\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      in 58.6% thyroid cancer samples compared to other cancer types (\n                      <jats:italic>p=0.00038<\/jats:italic>\n                      ). Immune cell fraction analysis between thyroid cancer samples with high and low\n                      <jats:italic>Cutibacterium<\/jats:italic>\n                      loads identify enrichment of immunosuppressive cells, including Tregs (\n                      <jats:italic>p=0.015<\/jats:italic>\n                      ), and other anti-inflammatory cytokines in the tumour microenvironment, suggesting an immune evasion\/immunosuppression\n                      <jats:italic>milieu<\/jats:italic>\n                      is associated with the infection. A higher burden of\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      was also found to be associated with poor survival defining a distinct sub-group of thyroid cancer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      is associated with immune suppression and poor prognosis in a subpopulation of thyroid cancer. This study may help design novel therapeutic measures involving appropriate antibiotics to manage the disease better.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2023.1152514","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T07:20:31Z","timestamp":1699860031000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Association of Cutibacterium acnes with human thyroid cancer"],"prefix":"10.3389","volume":"14","author":[{"given":"Vaishakhi","family":"Trivedi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanita","family":"Noronha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peddagangannagari","family":"Sreekanthreddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanket","family":"Desai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Disha","family":"Poojary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linu","family":"Varghese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Gowda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwin","family":"Butle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohit","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munita","family":"Bal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Mittal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swapnil","family":"Rane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubhada","family":"Kane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandip","family":"Basu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandini","family":"Menon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj","family":"Chaturvedi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pratik","family":"Chandrani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Choughule","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya","family":"Veldore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kumar","family":"Prabhash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Dutt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1001\/jamanetworkopen.2020.8759","article-title":"Global burden of thyroid cancer from 1990 to 2017","volume":"3","author":"Deng","year":"2020","journal-title":"JAMA Netw Open"},{"key":"B2","doi-asserted-by":"crossref","DOI":"10.5772\/intechopen.70128","article-title":"Thyroid Cancers: Considerations, Classifications, and Managements","volume-title":"Akarslan Z, editor. Diagnosis and Management of Head and Neck Cancer","author":"Khosravi","year":"2017"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1586\/14737140.9.3.317","article-title":"Contemporary management of papillary carcinoma of the thyroid gland","volume":"9","author":"Rosenbaum","year":"2009","journal-title":"Expert Rev Anticancer Ther"},{"key":"B4","article-title":"Anaplastic thyroid cancer: prevalence, diagnosis and treatment","volume":"33","author":"Chiacchio","year":"2008","journal-title":"Minerva Endocrinologica"},{"key":"B5","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1158\/2159-8290.CD-21-1059","article-title":"Hallmarks of cancer: new dimensions","volume":"12","author":"Hanahan","year":"2022","journal-title":"Cancer Discov"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J Clin"},{"key":"B7","doi-asserted-by":"crossref","DOI":"10.1111\/j.1572-0241.2005.41688.x","article-title":"Nyr\u00e9n Ol tLHpGCTF. Helicobacter pylori eradication has the potential to prevent gastric cancer a state-of-the-art critique","volume":"100","author":"Malfertheiner","year":"2005","journal-title":"Am J Gastroenterol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-019-0602-7","article-title":"Human papillomavirus as a driver of head and neck cancers","volume":"122","author":"Sabatini MEaC","year":"2020","journal-title":"Br J Cancer"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910520513","article-title":"The causal link between human papillomavirus and invasive cervical cancer: a population-based case-control study in Colombia and Spain","volume":"52","author":"Munoz","year":"1992","journal-title":"Int J Cancer"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.11.3265","article-title":"DNA of Epstein-Barr virus detected in tissue of Burkitt's lymphoma and nasopharyngeal carcinoma","volume":"70","author":"Nonoyama","year":"1973","journal-title":"Proc Natl Acad Sci USA"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/narcan\/zcac006","article-title":"Fusobacterium nucleatum is associated with inflammation and poor survival in early-stage HPV-negative tongue cancer","volume":"4","author":"Desai","year":"2022","journal-title":"NAR Cancer"},{"key":"B12","first-page":"1","article-title":"Bacterial signatures and their inflammatory potentials associated with prostate cancer","author":"Br\u00fcggemann","year":"2020","journal-title":"J Pathol Microbiol Immunol"},{"key":"B13","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.ijmm.2010.08.014","article-title":"Prevalence of Propionibacterium acnes in diseased prostates and its inflammatory and transforming activity on prostate epithelial cells","volume":"301","author":"Fassi Fehri","year":"2011","journal-title":"Int J Med Microbiol"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbaa437","article-title":"An integrated approach to determine the abundance, mutation rate and phylogeny of the SARS-CoV-2 genome","volume":"22","author":"Desai","year":"2021","journal-title":"Brief Bioinform"},{"key":"B15","unstructured":"WMA declaration of Helsinki \u2013 ethical principles for medical research involving human subjects2013"},{"key":"B16","volume-title":"Morpheus Web tool, Broad Institute, USA","year":""},{"key":"B17","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1186\/s13059-019-1891-0","article-title":"Improved metagenomic analysis with Kraken 2","volume":"20","author":"Wood","year":"2019","journal-title":"Genome Biol"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197","article-title":"Salmon: fast and bias-aware quantification of transcript expression using dual-phase inference","volume":"14","author":"Rob Patro","year":"2017","journal-title":"Nat Methods"},{"key":"B19","volume-title":"FastQC: a quality control tool for high throughput sequence data","author":"Andrews","year":"2010"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354","article-title":"MultiQC: summarize analysis results for multiple tools and samples in a single report","volume":"32","author":"Ewels","year":"2016","journal-title":"Bioinformatics"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"B22","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3337","article-title":"Robust enumeration of cell subsets from tissue expression profiles","volume":"12","author":"Newman","year":"2015","journal-title":"Nat Methods"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01274-5","article-title":"Systematic investigation of cytokine signalling activity at the tissue and single-cell level","volume":"18","author":"Jiang","year":"2021","journal-title":"Nat Methods"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00594-19","article-title":"Cutibacterium acnes (Propionibacterium acnes) 16S rRNA Genotyping of Microbial Samples from Possessions Contributes to Owner Identification","volume":"4","author":"Yang","year":"2019","journal-title":"mSystems"},{"key":"B26","volume-title":"survminer: Survival Analysis and Visualization","author":"Kassambara","year":"2017"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22136995","article-title":"Macrophage polarization states in the tumour microenvironment","volume":"22","author":"Boutilier","year":"2021","journal-title":"Int J Mol Sci"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.571334","article-title":"Cutibacterium acnes Infection Induces Type I Interferon Synthesis Through the cGAS-STING Pathway","volume":"11","author":"Fischer","year":"2020","journal-title":"Front Immunol"},{"key":"B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/microorganisms9020303","article-title":"Cutibacterium acnes as an opportunistic pathogen: an update of its virulence-associated factors","volume":"9","author":"Mayslich","year":"2021","journal-title":"Microorganisms"},{"key":"B30","first-page":"51","article-title":"Microbe-microbe and host-microbe interactions drive microbiome dysbiosis and inflammatory processes","volume":"23","author":"Proal","year":"2017","journal-title":"Discov Med"},{"key":"B31","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.chom.2020.12.001","article-title":"The cancer microbiome atlas: a pan-cancer comparative analysis to distinguish tissue-resident microbiota from contaminants","volume":"29","author":"Dohlman","year":"2021","journal-title":"Cell Host Microbe"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0558-9","article-title":"The landscape of viral associations in human cancers","volume":"52","author":"Zapatka","year":"2020","journal-title":"Nat Genet"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00092-13","article-title":"Propionibacterium acnes: from commensal to opportunistic biofilm-associated implant pathogen","volume":"27","author":"Achermann","year":"2014","journal-title":"Clin Microbiol Rev"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7050128","article-title":"Propionibacterium acnes and acne vulgaris: new insights from the integration of population genetic, multi-omic, biochemical and host-microbe studies","volume":"7","author":"McLaughlin","year":"2019","journal-title":"Microorganisms"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1586\/eri.11.137","article-title":"Propionibacterium acnes: infection beyond the skin","volume":"9","author":"Perry","year":"2011","journal-title":"Expert Rev Anti Infect Ther"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1556\/1886.2016.00040","article-title":"Propionibacterium acnes phylogenetic type iii is associated with progressive macular hypomelanosis","author":"Rolf","year":"2017","journal-title":"Eur J Microbiol Immunol"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169984","article-title":"Frequency of propionibacterium acnes infection in prostate glands with negative biopsy results is an independent risk factor for prostate cancer in patients with increased serum PSA titers","volume":"12","author":"Kakegawa","year":"2017","journal-title":"PloS One"},{"key":"B38","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s13027-018-0204-7","article-title":"The influence of prostatic Cutibacterium acnes infection on serum levels of IL6 and CXCL8 in prostate cancer patients","volume":"13","author":"Ugge","year":"2018","journal-title":"Infect Agent Cancer"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-018-1831-x","article-title":"Dysbiosis of the gut microbiome is associated with thyroid cancer and thyroid nodules and correlated with clinical index of thyroid function","author":"J Zhang","year":"2018","journal-title":"Endocrine"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2021.03.032","article-title":"The intratumor microbiome predicts prognosis across gender and subtypes in papillary thyroid carcinoma","volume":"19","author":"Gnanasekar","year":"2021","journal-title":"Comput Struct Biotechnol J"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01497-21","article-title":"Cutibacterium acnes induces the expression of immunosuppressive genes in macrophages and is associated with an increase of regulatory T-cells in prostate cancer","volume":"9","author":"Davidsson","year":"2021","journal-title":"Microbiol Spectr"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1213633","article-title":"Th17 cells and Tregs: unlikely allies","volume":"95","author":"Chen","year":"2014","journal-title":"J Leukoc Biol"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-018-0004-4","article-title":"When worlds collide: Th17 and Treg cells in cancer and autoimmunity","volume":"15","author":"Knochelmann","year":"2018","journal-title":"Cell Mol Immunol"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.11.003","article-title":"Contamination in low microbial biomass microbiome studies: issues and recommendations","volume":"27","author":"Eisenhofer","year":"2019","journal-title":"Trends Microbiol"},{"key":"B45","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1186\/s40168-023-01477-2","article-title":"Using strain-resolved analysis to identify contamination in metagenomics data","volume":"11","author":"Lou","year":"2023","journal-title":"Microbiome"},{"key":"B46","doi-asserted-by":"crossref","DOI":"10.1099\/mgen.0.001088","article-title":"Caution regarding the specificities of pan-cancer microbial structure","volume-title":"Microb Genom","author":"Gihawi","year":"2023"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: architecture and applications","volume":"10","author":"Camacho","year":"2009","journal-title":"BMC Bioinf"}],"container-title":["Frontiers in Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fendo.2023.1152514\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T07:20:38Z","timestamp":1699860038000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fendo.2023.1152514\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,10]]},"references-count":47,"alternative-id":["10.3389\/fendo.2023.1152514"],"URL":"https:\/\/doi.org\/10.3389\/fendo.2023.1152514","relation":{},"ISSN":["1664-2392"],"issn-type":[{"value":"1664-2392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,10]]},"article-number":"1152514"}}