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Endocrinol."],"abstract":"<jats:sec><jats:title>Introduction<\/jats:title><jats:p>Intracellular communication is essential for the maintenance of the anterior pituitary gland plasticity. The aim of this study was to evaluate whether GPCR-G\u03b1i modulates basic fibroblast growth factor (FGF2)-induced proliferative activity in normal pituitary cell populations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Anterior pituitary primary cell cultures from Wistar female rats were treated with FGF2 (10ng\/mL) or somatostatin analog (SSTa, 100nM) alone or co-incubated with or without the inhibitors of GPCR-G\u03b1i, pertussis toxin (PTX, 500nM), MEK inhibitor (U0126, 100\u00b5M) or PI3K inhibitor (LY 294002, 10 \u03bcM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FGF2 increased and SSTa decreased the lactotroph and somatotroph BrdU uptak2e (p&amp;lt;0.05) whereas the FGF2-induced S-phase entry was prevented by SSTa co-incubation in both cell types, with these effects being reverted by PTX, U0126 or LY294002 pre-incubation. The inhibition of lactotroph and somatotroph mitosis was associated with a downregulation of c-Jun expression, a decrease of phosphorylated (p) ERK and pAKT. Furthermore, SSTa was observed to inhibit the S-phase entry induced by FGF2, resulting in a further increase in the number of cells in the G1 phase and a concomitant reduction in the number of cells in the S phases (p&amp;lt; 0.05), effects related to a decrease of cyclin D1 expression and an increase in the expression of the cell cycle inhibitors p27 and p21.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>In summary, the GPCR-G\u03b1i activated by SSTa blocked the pro-proliferative effect of FGF2 in normal pituitary cells <jats:italic>via<\/jats:italic> a MEK-dependent mechanism, which acts as a mediator of both anti and pro-mitogenic signals, that may regulate the principal effectors of the G1 to S-phase transition.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2023.1183151","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T20:59:26Z","timestamp":1690491566000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulation of FGF2-induced proliferation by inhibitory GPCR in normal pituitary cells"],"prefix":"10.3389","volume":"14","author":[{"given":"Liliana del V.","family":"Sosa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florencia","family":"Picech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Perez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvina","family":"Gutierrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo 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