{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:34:44Z","timestamp":1792611284214,"version":"4.5.0"},"reference-count":121,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:p>This study aimed to identify key genes related to focal adhesions (FA) and cells involved in osteoblast (OS) and adipocyte (AD) differentiation in osteoporosis. A mouse model of disuse osteoporosis was made by hindlimbs unloading (HLU)\/Tail - suspension. Micro - CT and histological analysis were done, and differentially expressed genes (DEGs) from GSE100930 were analyzed. Soft clustering on GSE80614 OS\/AD samples found FA - related candidate genes. protein-protein interaction (PPI) network and cytoHubba\u2019s Degree algorithm identified key FA - genes, validated by quantitative polymerase chain reaction (qPCR). Key OS\/AD - associated cells were identified by single - cell analysis. The mouse model showed decreased bone density, microstructure damage, increased marrow adiposity, and altered gene expression. Key FA - related genes for osteogenesis (ITGB3, LAMC1, COL6A3, ITGA8, PDGFRB) and adipogenesis (ITGB3, ITGA4, LAMB1, ITGA8, LAMA4) were found and validated. Key cells (chondrocyte, adipocyte, and osteoblast progenitors) are involved in specific pathways, with osteoblast progenitors having stronger interactions. Pseudotime analysis implies differentiation from chondrocyte progenitors to adipocyte, then osteoblast progenitors. This study provides new insights for disuse osteoporosis research.<\/jats:p>","DOI":"10.3389\/fendo.2025.1469400","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T05:15:10Z","timestamp":1741583710000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["The potential key genes within focal adhesion that regulate mesenchymal stem cells osteogenesis or adipogenesis in microgravity related disuse osteoporosis: an integrated analysis"],"prefix":"10.3389","volume":"16","author":[{"given":"Haoyang","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Tu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200003000-00016","article-title":"Osteoporosis","volume":"372","author":"Lane","year":"2000","journal-title":"Clin Orthop Relat Res"},{"key":"B2","doi-asserted-by":"publisher","first-page":"46","DOI":"10.5152\/eurjrheum.2016.048","article-title":"An overview and management of osteoporosis","volume":"4","author":"Sozen","year":"2017","journal-title":"Eur J Rheumatol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1007\/s00198-023-06907-3","article-title":"Global burden of hip fracture: The Global Burden of Disease Study","volume":"35","author":"Feng","year":"2024","journal-title":"Osteoporos Int"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5411.143","article-title":"Multilineage potential of adult human mesenchymal stem cells","volume":"284","author":"Pittenger","year":"1999","journal-title":"Science"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2004.03.002","article-title":"Controlling the balance between osteoblastogenesis and adipogenesis and the consequent therapeutic implications","volume":"4","author":"Nuttall","year":"2004","journal-title":"Curr Opin Pharmacol"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020360","article-title":"Mesenchymal stem cells: cell fate decision to osteoblast or adipocyte and application in osteoporosis treatment","volume":"19","author":"Hu","year":"2018","journal-title":"IJMS"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.554565","article-title":"Proliferation and osteogenic differentiation of hMSCs on biomineralized collagen","volume":"8","author":"De Melo Pereira","year":"2020","journal-title":"Front Bioeng Biotechnol"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13011","article-title":"Region-specific effects of oestradiol on adipose-derived stem cell differentiation in post-menopausal women","volume":"21","author":"Cox-York","year":"2017","journal-title":"J Cell Mol Medi"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12131","article-title":"Adipogenic differentiation potential of adipose-derived mesenchymal stem cells from ovariectomized mice","volume":"47","author":"Fu","year":"2014","journal-title":"Cell Proliferation"},{"key":"B10","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1111\/j.1463-1326.2011.01488.x","article-title":"Oestrogen alters adipocyte biology and protects female mice from adipocyte inflammation and insulin resistance","volume":"14","author":"Stubbins","year":"2012","journal-title":"Diabetes Obes Metab"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9728.2004.00127.x","article-title":"Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma\/stem cells: the role of PPAR-\u03b32 transcription factor and TGF-\u03b2\/BMP signaling pathways: Aging alters the differentiation potential of mMSC, E. 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