{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T12:50:57Z","timestamp":1793019057982,"version":"4.5.0"},"reference-count":81,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023M731309"],"award-info":[{"award-number":["2023M731309"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:p>Non-obstructive azoospermia (NOA) constitutes one of the most severe forms of male infertility. Recent advancements in single-cell sequencing have significantly contributed to understanding the molecular landscape of NOA in human testicular tissues, elucidating the factors that underpin spermatogenic dysfunction. This technology has improved our understanding of the condition at a cellular level. Concurrently, bioinformatics developments have facilitated the re-analysis of publicly available single-cell datasets, offering novel insights into the disorder. Nevertheless, a comprehensive review integrating primary and re-analysis studies of single-cell sequencing in NOA is lacking. This review systematically evaluates 10 primary studies reporting original single-cell sequencing data of human NOA testicular samples and 22 secondary studies that re-analyzed these published data. We explore single-cell sequencing applications in germ cells, Sertoli cells, and Leydig cells, offering a comprehensive overview of molecular insights into spermatogenic dysfunction. Our review highlights novel findings in secondary studies, including the roles of transcriptional regulators, RNA transcription, endocrine disruptors, and microtubular cytoskeleton, thereby bridging primary studies and re-analysis studies. Additionally, we discussed future research directions and the challenges of translating single-cell research findings into clinical applications. In summary, single-cell sequencing offers a high-resolution, single-cell perspective of NOA testicular tissue, paving the way for innovative therapeutic strategies in male infertility.<\/jats:p>","DOI":"10.3389\/fendo.2025.1539063","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T05:16:53Z","timestamp":1742361413000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Single-cell sequencing in non-obstructive azoospermia: insights from primary and re-analysis studies"],"prefix":"10.3389","volume":"16","author":[{"given":"Zesong","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongjian","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmeng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiguo","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-020-02112-y","article-title":"Monogenic causes of non-obstructive azoospermia: challenges, established knowledge, limitations and perspectives","volume":"140","author":"Kasak","year":"2021","journal-title":"Hum Genet"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey116","article-title":"et al: Testicular microbiome in azoospermic men-first evidence of the impact of an altered microenvironment","volume":"33","author":"Alfano","year":"2018","journal-title":"Hum Reprod"},{"key":"B3","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/s12522-015-0212-x","article-title":"Predictive factors of successful sperm retrieval on microdissection testicular sperm extraction in Japanese men","volume":"15","author":"Enatsu","year":"2016","journal-title":"Reprod Med Biol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"3264","DOI":"10.3390\/ijms22063264","article-title":"Genetics of azoospermia","volume":"22","author":"Cioppi","year":"2021","journal-title":"Int J Mol Sci"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2024.1394812","article-title":"Understanding testicular single cell transcriptional atlas: from developmental complications to male infertility","volume":"15","author":"Tirumalasetty","year":"2024","journal-title":"Front Endocrinol (Lausanne)"},{"key":"B6","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1038\/s41368-021-00146-0","article-title":"From bulk, single-cell to spatial RNA sequencing","volume":"13","author":"Li","year":"2021","journal-title":"Int J Oral Sci"},{"key":"B7","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1016\/j.stem.2018.08.007","article-title":": Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis","volume":"23","author":"Wang","year":"2018","journal-title":"Cell Stem Cell"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-018-0099-2","article-title":": The adult human testis transcriptional cell atlas","volume":"28","author":"Guo","year":"2018","journal-title":"Cell Res"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.82191","article-title":"Application of single-cell RNA sequencing on human testicular samples: a comprehensive review","volume":"19","author":"Dong","year":"2023","journal-title":"Int J Biol Sci"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioac109","article-title":"Multiomics analysis of male infertility\u2020","volume":"107","author":"Wu","year":"2022","journal-title":"Biol Reprod"},{"key":"B11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/humrep\/deac245","article-title":"Human spermatogonial stem cells and their niche in male (in)fertility: novel concepts from single-cell RNA-sequencing","volume":"38","author":"Di Persio","year":"2023","journal-title":"Hum Reprod"},{"key":"B12","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1038\/s41572-023-00459-w","article-title":"Male infertility","volume":"9","author":"Eisenberg","year":"2023","journal-title":"Nat Rev Dis Primers"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.09.012","article-title":"Management of nonobstructive azoospermia: a committee opinion","volume":"110","year":"2018","journal-title":"Fertil Steril"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2019.02.013","article-title":"The management of obstructive azoospermia: a committee opinion","volume":"111","year":"2019","journal-title":"Fertil Steril"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.v12.3","article-title":"Single-cell RNA sequencing technologies and applications: A brief overview","volume":"12","author":"Jovic","year":"2022","journal-title":"Clin Transl Med"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1315","article-title":": mRNA-Seq whole-transcriptome analysis of a single cell","volume":"6","author":"Tang","year":"2009","journal-title":"Nat Methods"},{"key":"B17","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1016\/j.stem.2019.12.005","article-title":": the dynamic transcriptional cell atlas of testis development during human puberty","volume":"26","author":"Guo","year":"2020","journal-title":"Cell Stem Cell"},{"key":"B18","doi-asserted-by":"publisher","first-page":"5683","DOI":"10.1038\/s41467-020-19414-4","article-title":": Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells","volume":"11","author":"Zhao","year":"2020","journal-title":"Nat\u00a0Commun"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.768834","article-title":"Progress of single-cell RNA sequencing technology in myocardial infarction research","volume":"9","author":"Li","year":"2022","journal-title":"Front Cardiovasc Med"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-0502-3_12","article-title":"Emergence of Bias During the Synthesis and Amplification of cDNA for scRNA-seq","volume":"1068","author":"Luo","year":"2018","journal-title":"Adv Exp Med Biol"},{"key":"B21","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.362","article-title":"What has single-cell RNA sequencing revealed about microglial neuroimmunology","volume":"8","author":"Kubick","year":"2020","journal-title":"Immun Inflammation Dis"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.04.005","article-title":"The technology and biology of single-cell RNA sequencing","volume":"58","author":"Kolodziejczyk","year":"2015","journal-title":"Mol Cell"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00317","article-title":"Single-cell RNA-seq technologies and related computational data analysis","volume":"10","author":"Chen","year":"2019","journal-title":"Front Genet"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1049\/nbt2.12115","article-title":"scRNA-seq data analysis method to improve analysis performance","volume":"17","author":"Lu","year":"2023","journal-title":"IET Nanobiotechnol"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202206852","article-title":"Deciphering the molecular characteristics of human idiopathic nonobstructive azoospermia from the perspective of germ cells","volume":"10","author":"Chen","year":"2023","journal-title":"Adv Sci (Weinh)"},{"key":"B26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/stmcls\/sxad077","article-title":"Single-cell RNA sequencing technology landscape in 2023","volume":"42","author":"Qu","year":"2024","journal-title":"Stem Cells"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-023-01232-7","article-title":": Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination","volume":"25","author":"Huang","year":"2023","journal-title":"Nat Cell Biol"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcae009","article-title":"Long-read single-cell sequencing reveals the transcriptional landscape of spermatogenesis in obstructive azoospermia and Sertoli cell-only patients","volume":"117","author":"Wu","year":"2024","journal-title":"Qjm"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2021.09.001","article-title":": Comparative single-cell analysis of biopsies clarifies pathogenic mechanisms in Klinefelter syndrome","volume":"108","author":"Mahyari","year":"2021","journal-title":"Am J Hum Genet"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.7150\/thno.55645","article-title":": Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis","volume":"11","author":"Wang","year":"2021","journal-title":"Theranostics"},{"key":"B31","doi-asserted-by":"publisher","first-page":"5205","DOI":"10.1038\/s41467-021-25544-0","article-title":": Aging, inflammation and DNA damage in the somatic testicular niche with idiopathic germ cell aplasia","volume":"12","author":"Alfano","year":"2021","journal-title":"Nat Commun"},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/humrep\/13.suppl_1.1","article-title":"Spermatogenesis","author":"de Kretser","year":"1998","journal-title":"Hum Reprod"},{"key":"B33","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.semcdb.2016.04.009","article-title":"Spermatogenesis in humans and its affecting factors","volume":"59","author":"Neto","year":"2016","journal-title":"Semin Cell Dev Biol"},{"key":"B34","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.1016\/j.devcel.2022.04.004","article-title":": Single-cell analysis of human testis aging and correlation with elevated body mass index","volume":"57","author":"Nie","year":"2022","journal-title":"Dev Cell"},{"key":"B35","doi-asserted-by":"publisher","first-page":"3610466","DOI":"10.1155\/2023\/3610466","article-title":"MAGEB2-mediated degradation of EGR1 regulates the proliferation and apoptosis of human spermatogonial stem cell lines","volume":"2023","author":"Zhao","year":"2023","journal-title":"Stem Cells Int"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.4103\/aja202254","article-title":"FOXP4 promotes proliferation of human spermatogonial stem cells","volume":"25","author":"Luo","year":"2023","journal-title":"Asian J Androl"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v14.i12.822","article-title":"SPOC domain-containing protein 1 regulates the proliferation and apoptosis of human spermatogonial stem cells through adenylate kinase 4","volume":"14","author":"Zhou","year":"2022","journal-title":"World J Stem Cells"},{"key":"B38","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/path.v221:1","article-title":"Autophagy: cellular and molecular mechanisms","volume":"221","author":"Glick","year":"2010","journal-title":"J\u00a0Pathol"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.176","article-title":"S phase-dependent interaction with DNMT1 dictates the role of UHRF1 but not UHRF2 in DNA methylation maintenance","volume":"21","author":"Zhang","year":"2011","journal-title":"Cell Res"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1242\/dev.107029","article-title":": Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis","volume":"141","author":"Rebourcet","year":"2014","journal-title":"Development"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.1012119","article-title":"Testicular aging, male fertility and beyond","volume":"13","author":"Dong","year":"2022","journal-title":"Front Endocrinol (Lausanne)"},{"key":"B42","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.semcdb.2014.02.011","article-title":"Sertoli cells\u2013immunological sentinels of spermatogenesis","volume":"30","author":"Kaur","year":"2014","journal-title":"Semin Cell Dev Biol"},{"key":"B43","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.semcdb.2021.06.016","article-title":"Sertoli cells as key drivers of testis function","volume":"121","author":"O\u2019Donnell","year":"2022","journal-title":"Semin Cell Dev Biol"},{"key":"B44","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.metabol.2017.11.018","article-title":"The hypothalamus-pituitary-gonad axis: Tales of mice and men","volume":"86","author":"Kaprara","year":"2018","journal-title":"Metabolism"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00224","article-title":"Molecular mechanisms and signaling pathways involved in sertoli cell proliferation","volume":"10","author":"Meroni","year":"2019","journal-title":"Front Endocrinol (Lausanne)"},{"key":"B46","doi-asserted-by":"publisher","first-page":"112449","DOI":"10.1016\/j.biopha.2021.112449","article-title":": Single-cell transcriptome analysis of Bisphenol A exposure reveals the key roles of the testicular microenvironment in male reproduction","volume":"145","author":"Zhang","year":"2022","journal-title":"BioMed Pharmacother"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2020.09.004","article-title":"Human obstructive (postvasectomy) and nonobstructive azoospermia - Insights from scRNA-Seq and transcriptome analysis","volume":"9","author":"Chen","year":"2022","journal-title":"Genes Dis"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1705-0","article-title":"Single-cell transcriptome analysis of the novel coronavirus (SARS-CoV-2) associated gene ACE2 expression in normal and non-obstructive azoospermia (NOA) human male testes","volume":"63","author":"Liu","year":"2020","journal-title":"Sci China Life Sci"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.v24.16","article-title":"The ACE2 expression in Sertoli cells and germ cells may cause male reproductive disorder after SARS-CoV-2 infection","volume":"24","author":"Shen","year":"2020","journal-title":"J Cell Mol Med"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03101-9","article-title":"The roles and mechanisms of Leydig cells and myoid cells in regulating spermatogenesis","volume":"76","author":"Zhou","year":"2019","journal-title":"Cell Mol Life Sci"},{"key":"B51","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1186\/s13578-023-01034-2","article-title":"Identification and validation of CCL2 as a potential biomarker relevant to mast cell infiltration in the testicular immune microenvironment of spermatogenic dysfunction","volume":"13","author":"Dong","year":"2023","journal-title":"Cell Biosci"},{"key":"B52","doi-asserted-by":"publisher","first-page":"8819","DOI":"10.3390\/ijms24108819","article-title":"Macrophage-related testicular inflammation in individuals with idiopathic non-obstructive azoospermia: A single-cell analysis","volume":"24","author":"Xia","year":"2023","journal-title":"Int J Mol Sci"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203675","article-title":"Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue","volume":"13","author":"Zhou","year":"2021","journal-title":"Aging (Albany NY)"},{"key":"B54","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1186\/s13059-020-1934-6","article-title":"Lisa: inferring transcriptional regulators through integrative modeling of public chromatin accessibility and ChIP-seq data","volume":"21","author":"Qin","year":"2020","journal-title":"Genome Biol"},{"key":"B55","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.01.029","article-title":"The human transcription factors","volume":"172","author":"Lambert","year":"2018","journal-title":"Cell"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-022-01008-8","article-title":"A 3-gene random forest model to diagnose non-obstructive azoospermia based on transcription factor-related henes","volume":"30","author":"Zhou","year":"2023","journal-title":"Reprod Sci"},{"key":"B57","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1138386","article-title":"Single-cell multi-omics analysis reveals dysfunctional Wnt signaling of spermatogonia in non-obstructive azoospermia","volume":"14","author":"Zeng","year":"2023","journal-title":"Front Endocrinol (Lausanne)"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.875762","article-title":"Single-cell transcriptomics-based study of transcriptional regulatory features in the non-obstructive azoospermia testis","volume":"13","author":"Tang","year":"2022","journal-title":"Front Genet"},{"key":"B59","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1186\/s12864-016-2618-1","article-title":"Transcriptome analysis of highly purified mouse spermatogenic cell populations: gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage","volume":"17","author":"da Cruz","year":"2016","journal-title":"BMC Genomics"},{"key":"B60","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00679-09","article-title":"Loss of YY1 impacts the heterochromatic state and meiotic double-strand breaks during mouse spermatogenesis","volume":"29","author":"Wu","year":"2009","journal-title":"Mol Cell Biol"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.24272\/j.issn.2095-8137.2023.414","article-title":"Meiotic transcriptional reprogramming mediated by cell-cell communications in humans and mice revealed by scATAC-seq and scRNA-seq","volume":"45","author":"Wang","year":"2024","journal-title":"Zool Res"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204674","article-title":"An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes","volume":"15","author":"Peng","year":"2023","journal-title":"Aging (Albany NY)"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.18632\/aging.204749","article-title":"Identification and validation of diagnostic signature genes in non-obstructive azoospermia by machine learning","volume":"15","author":"Ran","year":"2023","journal-title":"Aging (Albany NY)"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.18632\/aging.202559","article-title":"Infertility network and hub genes for nonobstructive azoospermia utilizing integrative analysis","volume":"13","author":"Han","year":"2021","journal-title":"Aging (Albany NY)"},{"key":"B65","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1186\/s12864-024-10506-9","article-title":"Whole transcriptome analysis to identify non-coding RNA regulators and hub genes in sperm of non-obstructive azoospermia by microarray, single-cell RNA sequencing, weighted gene co-expression network analysis, and mRNA-miRNA-lncRNA interaction analysis","volume":"25","author":"Karoii","year":"2024","journal-title":"BMC Genomics"},{"key":"B66","doi-asserted-by":"publisher","first-page":"55","DOI":"10.21037\/atm-22-5601","article-title":"Identification of biomarkers associated with macrophage infiltration in non-obstructive azoospermia using single-cell transcriptomic and microarray data","volume":"11","author":"Luo","year":"2023","journal-title":"Ann Trans Med"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13098","article-title":": RNA-binding protein ELAVL2 plays post-transcriptional roles in the regulation of spermatogonia proliferation and apoptosis","volume":"54","author":"Yang","year":"2021","journal-title":"Cell Prolif"},{"key":"B68","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-023-02892-y","article-title":"Identification of a novel pyroptosis-related gene signature in human spermatogenic dysfunction","volume":"40","author":"Dong","year":"2023","journal-title":"J Assist Reprod Genet"},{"key":"B69","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2013.08.008","article-title":"Bisphenol A and human health: a review of the literature","volume":"42","author":"Rochester","year":"2013","journal-title":"Reprod Toxicol"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.936738","article-title":"Hair sample analysis of residents from olsztyn, northeastern Poland, to evaluate levels of bisphenol S and bisphenol A: A pilot study","volume":"28","author":"Gonkowski","year":"2022","journal-title":"Med Sci Monit"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.04.177","article-title":"Identification of genetic networks involved in the cell injury accompanying endoplasmic reticulum stress induced by bisphenol A in testicular Sertoli cells","volume":"345","author":"Tabuchi","year":"2006","journal-title":"Biochem Biophys Res Commun"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2007.07.010","article-title":"Human exposure to bisphenol A (BPA)","volume":"24","author":"Vandenberg","year":"2007","journal-title":"Reprod Toxicol"},{"key":"B73","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.semcdb.2014.01.003","article-title":"Microtubules and spermatogenesis","volume":"30","author":"O\u2019Donnell","year":"2014","journal-title":"Semin Cell Dev Biol"},{"key":"B74","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1186\/s12958-022-01026-w","article-title":": Defects of microtubule cytoskeletal organization in NOA human testes","volume":"20","author":"Wu","year":"2022","journal-title":"Reprod Biol Endocrinol"},{"key":"B75","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13008-022-00077-0","article-title":"An updated view on the centrosome as a cell cycle regulator","volume":"17","author":"Lin","year":"2022","journal-title":"Cell Div"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140968","article-title":"Cep169, a novel microtubule plus-end-tracking centrosomal protein, binds to CDK5RAP2 and regulates microtubule stability","volume":"10","author":"Mori","year":"2015","journal-title":"PloS One"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2023.03.015","article-title":"A novel missense variant in CDK5RAP2 associated with non-obstructive azoospermia","volume":"62","author":"Rahimian","year":"2023","journal-title":"Taiwan J Obstet Gynecol"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.4161\/21565562.2014.979106","article-title":"Testicular histopathology associated with disruption of the Sertoli cell cytoskeleton","volume":"4","author":"Johnson","year":"2014","journal-title":"Spermatogenesis"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00370-8","article-title":"Integrating single-cell and spatial transcriptomics to elucidate intercellular tissue dynamics","volume":"22","author":"Longo","year":"2021","journal-title":"Nat Rev Genet"},{"key":"B80","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-05795-1","article-title":"et al: Spatial epigenome-transcriptome co-profiling of mammalian tissues","volume":"616","author":"Zhang","year":"2023","journal-title":"Nature"},{"key":"B81","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2021.07.011","article-title":"Role of laminin and collagen chains in human spermatogenesis - Insights from studies in rodents and scRNA-Seq transcriptome profiling","volume":"121","author":"Wu","year":"2022","journal-title":"Semin Cell Dev Biol"}],"container-title":["Frontiers in Endocrinology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fendo.2025.1539063\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T05:16:57Z","timestamp":1742361417000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fendo.2025.1539063\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,19]]},"references-count":81,"alternative-id":["10.3389\/fendo.2025.1539063"],"URL":"https:\/\/doi.org\/10.3389\/fendo.2025.1539063","relation":{},"ISSN":["1664-2392"],"issn-type":[{"value":"1664-2392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,19]]},"article-number":"1539063"}}