{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:14:41Z","timestamp":1770293681344,"version":"3.49.0"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013138","name":"Hainan Provincial Department of Health","doi-asserted-by":"publisher","award":["22A200273"],"award-info":[{"award-number":["22A200273"]}],"id":[{"id":"10.13039\/501100013138","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the impact of smooth endoplasmic reticulum aggregates (SERa) in oocytes on embryological outcomes and clinical and neonatal outcomes during split IVF-ICSI cycles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A retrospective analysis was conducted using clinical data from January 2020 to December 2023 at the Reproductive Medicine Center of Hainan Women and Children\u2019s Medical Center. Patients were divided into SERa+ and SERa- cycles based on the visibility of SERa after the removal of cumulus cells. Basic patient characteristics, embryological outcomes, clinical and neonatal outcomes were compared between the two groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to the SERa- cycles, the SERa+ cycles showed significantly higher levels of E<jats:sub>2<\/jats:sub> on the day of hCG administration (P&amp;lt;0.01) and a significantly increased number of retrieved oocytes (P&amp;lt;0.01). In terms of embryological outcomes, the total D3 high-quality embryo rate was significantly higher in the SERa+ cycles (P&amp;lt;0.01). There was a significant increase in the D3 high-quality embryo rate for ICSI, but no difference in the D3 high-quality embryo rate for IVF. No significant differences were observed between the SERa+ and SERa- cycles in terms of \u03b2hCG positivity rate, clinical pregnancy rate, implantation rate, early miscarriage rate, live birth rate, preterm birth rate, newborn height, and weight (P&amp;gt;0.05). No congenital birth defects were found in either group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The occurrence of SERa in split IVF-ICSI cycles may be associated with increased E<jats:sub>2<\/jats:sub> levels on hCG day, and the presence of SERa does not appear to affect <jats:italic>in vitro<\/jats:italic> fertilization, embryological, clinical, or neonatal outcomes.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fendo.2025.1567066","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T04:10:36Z","timestamp":1745899836000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Oocytes with aggregates of smooth endoplasmic reticulum may not affect reproductive outcomes in split IVF-ICSI insemination: a retrospective 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