{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:16:11Z","timestamp":1793812571214,"version":"4.6.1"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T00:00:00Z","timestamp":1768780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Estrogen signaling is essential for ovarian differentiation in vertebrates, but its developmental onset and specific roles during early gonadogenesis remain unclear. In medaka (\n                      <jats:italic>Oryzias latipes<\/jats:italic>\n                      ), the first morphological sex difference appears as a higher germ cell number in XX compared with XY embryos before hatching. Recently, we demonstrated that zygotically synthesized estrogen was dispensable for early germ cell sex difference but essential for subsequent oocyte meiotic progression and ovarian fate maintenance. Nevertheless, whether these phenomena depend on maternal estrogen or zygotic estrogen signaling mediated by nuclear estrogen receptors (nEsrs) is unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To clarify the receptor-level requirement of estrogen signaling, we generated\n                      <jats:italic>esr1\/2a\/2b<\/jats:italic>\n                      triple knockout (\u0394nEsrs) medaka using CRISPR\/Cas9 genome editing. By comparing these receptor-deficient mutants with our previous ligand-deficient (\u0394\n                      <jats:italic>cyp19a1a\/1b<\/jats:italic>\n                      double knockout) model, we aimed to determine whether estrogen signaling is involved in the establishment of the early germ cell number difference or acts later to control meiotic progression and ovarian maintenance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We established \u0394nEsrs medaka using CRISPR\/Cas9 and analyzed gonadal histology, germ cell kinetics, and expression of steroidogenic enzyme genes, sex differentiation-related genes, and oocyte-specific expressed genes during early development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      \u0394nEsrs mutants displayed normal early germ cell number and sex-specific differences at hatching (0 dph). At 10 dph, diplotene oocytes were markedly reduced, accompanied by significant downregulation of oocyte-specific genes,\n                      <jats:italic>figa<\/jats:italic>\n                      ,\n                      <jats:italic>42sp50<\/jats:italic>\n                      , as well as\n                      <jats:italic>cyp19a1a<\/jats:italic>\n                      and\n                      <jats:italic>foxl2<\/jats:italic>\n                      mRNA. However, \u0394nEsrs did not cause feedback regulation on other hypothalamus-pituitary-gonad (HPG) axis gene expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results demonstrate that estrogen signaling, both at the ligand and receptor levels, is dispensable for establishing the early germ cell sex difference but essential for subsequent oocyte meiotic progression and ovarian fate maintenance. This establishes a two-step estrogenic control model, redefining the developmental timing of estrogen action during the early phase of gonadal differentiation in vertebrate reproduction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2025.1769798","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:37:42Z","timestamp":1768829862000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Estrogenic control of germ cell differentiation in medaka: independence of early sex dimorphism from zygotic estrogen and receptor signaling"],"prefix":"10.3389","volume":"16","author":[{"given":"Yuta","family":"Sakai-Yamada","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka","place":["Shizuoka, Japan"]},{"name":"Graduate School of Pharmaceutical and Nutritional Sciences, University of 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Institute, The University of Tokyo","place":["Kashiwa, Chiba, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Reproductive Biology, Institute for Environmental Sciences, University of Shizuoka","place":["Shizuoka, Japan"]},{"name":"Graduate School of Pharmaceutical and Nutritional Sciences, University of Shizuoka","place":["Shizuoka, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1023\/a:1020068224909","article-title":"Estrogen receptors: structure, mechanisms and function","volume":"3","author":"Hewitt","year":"2002","journal-title":"Rev Endocr Metab Disord"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2009.03.026","article-title":"Mechanisms of estrogen signaling and gene 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