{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T04:47:08Z","timestamp":1776919628294,"version":"3.51.2"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T00:00:00Z","timestamp":1776902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Glyphosate-based herbicides (GBHs), such as Roundup\u00ae, are ubiquitous environmental contaminants with emerging endocrine-disrupting properties. Based on previous findings of non-monotonic effects in thyroid models, this study investigated lineage-dependent cytotoxic and proliferative responses to Roundup\u00ae in thyroid cells with different cytogenic profiles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human papillary carcinoma (BCPAP, BRAFV600E) and anaplastic carcinoma (8505C) cells were exposed to Roundup\u00ae Original DI (6.5\u20136500 \u00b5g\/L) for 24 and 48 hours. Cell viability was assessed using trypan blue exclusion and CCK-8 assays, and proliferation was measured by BrdU incorporation. The data were integrated with previous results from normal (Nthy-ori 3-1) and papillary (TPC-1) cell lines for comparative analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Exposure to Roundup\u00ae induced specific effects for each cell type. The BCPAP cell line demonstrated significant sensitivity, with reduced viability and sustained proliferative stimulation at low concentrations. In contrast, anaplastic 8505C cells proved to be highly resistant, exhibiting transient biphasic proliferation and recovery of viability even at high doses. A consolidated analysis established a clear hierarchy of sensitivity: TPC-1 &amp;gt; BCPAP &amp;gt; Nthy-ori3-1 &amp;gt; 8505C, revealing that papillary thyroid carcinoma cells are the most vulnerable. Crucially, a concentration of 65 \u00b5g\/L, which is below current regulatory limits, triggered proliferative responses in all cell models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These results suggest that Roundup\u00ae is a potent endocrine disruptor that exerts mutation-specific proliferative effects on thyroid cancer cells. Consistent growth stimulation at low, environmentally relevant doses suggests a potential tumor-promoting role, particularly in papillary carcinomas driven by the MAPK pathway. This evidence reinforces the need to revise risk assessments of GBHs (subcutaneous hypothyroidism) to take into account endocrine disruption and the potential for cancer progression beyond traditional toxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2026.1745247","type":"journal-article","created":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T04:22:25Z","timestamp":1776918145000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Roundup\u00ae Original DI promotes proliferation in papillary thyroid carcinoma cells: a comparative study highlighting cell-type-dependent effects"],"prefix":"10.3389","volume":"17","author":[{"given":"Izabela Fernanda","family":"Dal\u2019 B\u00f3","sequence":"first","affiliation":[{"name":"Laboratory of Cancer Molecular Genetics, School of Medical Sciences, University of Campinas (UNICAMP)","place":["Campinas, SP, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1ssia Elena","family":"Bufalo","sequence":"additional","affiliation":[{"name":"Laboratory of Cancer Molecular Genetics, School of Medical Sciences, University of Campinas (UNICAMP)","place":["Campinas, SP, Brazil"]},{"name":"Department of Medicine, S\u00e3o Leopoldo Mandic and Research Center","place":["Campinas, SP, Brazil"]},{"name":"Max-Planck University Center (UniMAX)","place":["Indaiatuba, SP, Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valdemar","family":"M\u00e1ximo","sequence":"additional","affiliation":[{"name":"Institute for Research and Innovation in Health (i3S), University of Porto","place":["Porto, Portugal"]},{"name":"Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup)","place":["Porto, Portugal"]},{"name":"Department of Pathology, Faculty of Medicine of the University of Porto (FMUP)","place":["Porto, 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