{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T05:01:18Z","timestamp":1789016478225,"version":"build-2803163510"},"reference-count":52,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2024-04456, RGPIN-2021-03174, RTI-2021-00643"],"award-info":[{"award-number":["RGPIN-2024-04456, RGPIN-2021-03174, RTI-2021-00643"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","award":["35859"],"award-info":[{"award-number":["35859"]}],"id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012171","name":"Ontario Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      The vertebrate nonapeptide vasotocin (Avp) is evolutionarily conserved and homologous to mammalian vasopressin. Previous work using\n                      <jats:italic>avp<\/jats:italic>\n                      <jats:sup>-\/-<\/jats:sup>\n                      zebrafish demonstrated a role for Avp in female, but not male, zebrafish reproduction. The phenotype manifested as reductions in pair-breeding success, viable egg release, ovarian Prostaglandin F\n                      <jats:sub>2a<\/jats:sub>\n                      (PGF\n                      <jats:sub>2a<\/jats:sub>\n                      ) concentration, and reduction in ovarian transcripts coding for PGF\n                      <jats:sub>2a<\/jats:sub>\n                      . synthesis enzymes. However, mutants exhibited life-long hypercortisolism, confounding the investigation of the mechanistic basis of Avp in female zebrafish reproductive success.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here, we used acute pharmacological manipulation via the Avpr1 receptor antagonist Manning compound alone (dose-response experiment) or in conjunction with PGF\n                      <jats:sub>2a<\/jats:sub>\n                      (rescue experiment) to investigate the role of Avp1-type receptor (Avpr1) signalling in female zebrafish reproductive success.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the dose-response experiment, acute female intraperitoneal injection of MC at the lowest dose tested (5 ng\/g bodyweight) reduced breeding pair success, quivering behaviour, and the number of released viable eggs compared to saline-injected controls. Whole-body cortisol and reproductive hormone concentrations, clutch size, hatchability and larval survival were unaffected at this concentration. Additional effects on courtship behaviours and increases in estradiol and progesterone concentrations were observed exclusively in females injected with higher doses of MC, suggesting no contribution to the reproductive phenotype observed at the lowest MC dose. The rescue experiment revealed that the phenotype elicited by 5 ng\/g bw MC was rescued by co-injection with 5 mg\/g bw PGF\n                      <jats:sub>2a<\/jats:sub>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      Together, these data suggest that Avp acutely promotes female zebrafish spawning through action on Avpr1 receptors and likely involve downstream activation of PGF\n                      <jats:sub>2a<\/jats:sub>\n                      . Because Avp may regulate female reproductive success at the ovarian level, future studies should explore the location, regulation, and function of the ovarian Avp receptors in detail.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2026.1771879","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T04:21:22Z","timestamp":1777522882000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Acute administration of Manning compound during the spawning period reduces reproductive success in female zebrafish"],"prefix":"10.3389","volume":"17","author":[{"given":"Dinusha","family":"Rajapaksha","sequence":"first","affiliation":[{"name":"Department of Biology, University of Ottawa","place":["Ottawa, ON, 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