{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T05:05:57Z","timestamp":1779944757136,"version":"3.53.1"},"reference-count":45,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["21JR7RA391, 21JR1RA101"],"award-info":[{"award-number":["21JR7RA391, 21JR1RA101"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009590","name":"Gansu Education Department","doi-asserted-by":"publisher","award":["2021B-011"],"award-info":[{"award-number":["2021B-011"]}],"id":[{"id":"10.13039\/501100009590","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016321","name":"First Clinical Medical School, Lanzhou University","doi-asserted-by":"publisher","award":["2019-96"],"award-info":[{"award-number":["2019-96"]}],"id":[{"id":"10.13039\/501100016321","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Previous studies on factors influencing embryonic aneuploidy have produced inconsistent results. This study aimed to identify predictors of\n                      <jats:italic>de novo<\/jats:italic>\n                      aneuploidy in distinct patients undergoing preimplantation genetic testing for structural chromosome rearrangements (PGT-SR), aneuploidy (PGT-A) and monogenic disorders (PGT-M).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analyzed associations between maternal age, paternal age, anti-M\u00fcllerian hormone (AMH), maternal body mass index (BMI), controlled ovarian stimulation (COS) protocol, sperm parameters, blastocyst expansion degree, inner cell mass (ICM) grade, trophectoderm (TE) grade, and\n                      <jats:italic>de novo<\/jats:italic>\n                      aneuploidy using generalized linear mixed models (GLMM).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The incidence of\n                      <jats:italic>de novo<\/jats:italic>\n                      aneuploidy was 19.5% (PGT-SR), 32.1% (PGT-A), and 31.2% (PGT-M). Multivariate analysis showed that advanced maternal age and blastocyst expansion degree were significant predictors in the PGT-SR cohort. In the PGT-A cohort, significant associations were observed for maternal age, AMH, PGT indication, expansion degree, ICM grade, and TE grade. In the PGT-M cohort, TE grade was significantly associated with aneuploidy in women aged \u2265 30 years. No independent effects were found for semen parameters or stimulation protocols in any cohort. Whole-chromosome aneuploidies most frequently involved chromosomes 22, 16, and 21. There were no dominant chromosomes for segmental-chromosome aneuploidy. Notably, the aneuploidy rate remained consistently low (~20%) in women aged \u2264 30 years.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In this study, advanced maternal age (\u226535 years) emerged as a robust predictor of\n                      <jats:italic>de novo<\/jats:italic>\n                      aneuploidy. The expansion degree ex hibited an association with\n                      <jats:italic>de novo<\/jats:italic>\n                      aneuploidy across both the PGT-SR and PGT-A cohorts. The TE grade seemed to be correlated with\n                      <jats:italic>de novo<\/jats:italic>\n                      aneuploidy, especially in the PGT-A cohort, and the TE grade exhibited stronger predictive performance compared to the ICM grade. No independent predictive effect was detected for semen parameters or stimulation protocols. These findings support the implementation of individualized risk assessment and embryo selection in distinct PGT patient cohorts.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2026.1805736","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T04:48:52Z","timestamp":1779943732000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Factors associated with de novo aneuploidy across different preimplantation genetic testing cycles: a retrospective cohort study"],"prefix":"10.3389","volume":"17","author":[{"given":"Hongfang","family":"Liu","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, The First Hospital of Lanzhou University","place":["Lanzhou, China"]},{"name":"Gansu Key Laboratory of Reproductive Medicine and Embryology","place":["Lanzhou, 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China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Mao","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The First Hospital of Lanzhou University","place":["Lanzhou, China"]},{"name":"The First Clinical Medical College, Lanzhou University","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Ma","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, The First Hospital of Lanzhou University","place":["Lanzhou, China"]},{"name":"Gansu Key Laboratory of Reproductive Medicine and Embryology","place":["Lanzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2023.08.952","article-title":"Genetic causes of sporadic and recurrent 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