{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:08:38Z","timestamp":1780376918964,"version":"3.54.1"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the intrinsic mechanism by which electroacupuncture (EA) treats embryo implantation dysfunction in mice from the perspective of exosomal miRNAs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Human trophoblast cells (HTR-8\/Svneo) were co-cultured\n                      <jats:italic>in vitro<\/jats:italic>\n                      with endometrial cells (Ishikawa and HEC-1-A), and exosomes derived from endometrial cells were isolated to treat HTR-8\/Svneo cells. Cell proliferation and invasion were assessed by EdU staining and Transwell assays. miRNA sequencing was performed on exosomes from the two endometrial cell lines to identify differentially expressed miRNAs. A mifepristone-induced mouse model of implantation dysfunction was established and treated with EA. The number of implantation sites was counted, pathological changes in the endometrium were observed by hematoxylin-eosin (HE) staining, and miR-30c-5p expression was detected by qPCR. miR-30c-5p was inhibited in HTR-8\/Svneo cells, and changes in cell proliferation, invasion, and phosphorylation of FAK and JNK were evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Communication existed between HTR-8\/Svneo cells and endometrial cells, and co-culture with Ishikawa cells significantly promoted the proliferation and invasion of HTR-8\/Svneo cells. Exosomes derived from both Ishikawa and HEC-1-A cells promoted HTR-8\/Svneo cell proliferation and invasion. High-throughput sequencing showed that miR-30c-5p was expressed at a low level in Ishikawa cell-derived exosomes but at a high level in HEC-1-A cell-derived exosomes.\n                      <jats:italic>In vivo<\/jats:italic>\n                      experiments demonstrated that EA reduced miR-30c-5p expression in the endometrium of mice with implantation dysfunction, showed a trend of increasing implantation sites, and alleviated endometrial injury. Inhibition of miR-30c-5p in HTR-8\/Svneo cells promoted cell proliferation and invasion and increased the phosphorylation of FAK and JNK.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>EA may ameliorate embryo implantation dysfunction in mice by downregulating miR-30c-5p in the endometrium.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2026.1847688","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T04:29:07Z","timestamp":1780374547000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Electroacupuncture ameliorates embryo implantation dysfunction in mice via miR-30c-5p"],"prefix":"10.3389","volume":"17","author":[{"given":"Jing-Yan","family":"Song","sequence":"first","affiliation":[{"name":"The First Clinical College, Shandong University of Traditional Chinese Medicine","place":["Jinan, China"]},{"name":"Reproductive Center of Integrated Medicine, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine","place":["Jinan, China"]},{"name":"Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore","place":["Singapore, Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Yi","family":"Dong","sequence":"additional","affiliation":[{"name":"Child Rehabilitation Center, Jinan Maternity and Child Care Hospital Affiliated to Shandong First Medical University","place":["Jinan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1016\/j.molmed.2020.12.008","article-title":"Strategies to identify genetic variants causing 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