{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T06:01:54Z","timestamp":1789970514682,"version":"4.0.1"},"reference-count":19,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010253","name":"Secretar\u00eda de Ciencia y T\u00e9cnica, Universidad de Buenos Aires","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010253","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Endocrinol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Conventional sperm motility analysis often fails to capture the dynamic physiological changes associated with fertilization. The identification of a transition hyperactivated (TH) phenotype, characterized by high vigor while preserving linearity, may provide additional information on ejaculate functional competence beyond standard semen parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To establish an objective method for detecting TH motility using computer-assisted sperm analysis (CASA) with 10-\u03bcm chambers and to evaluate its clinical predictive value in comparison with pharmacological induction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      The Sperm Class Analyzer (SCA) system (Microptic SL) was validated using the following kinematic thresholds: curvilinear velocity (VCL) &amp;gt; 35 \u03bcm\/s, linearity (LIN) &amp;gt; 50%, and amplitude of lateral head displacement (ALH) &amp;gt; 2.5 \u03bcm. Semen samples from fertile men (\n                      <jats:italic>n<\/jats:italic>\n                      =\u00a027) and men consulting for infertility (\n                      <jats:italic>n<\/jats:italic>\n                      =\u00a0229) were analyzed under different conditions. TH motility was assessed in ejaculated semen (THE), after swim-up (THS), and after overnight incubation (THO). The non-genomic effects of thyroxine (T4, 0.002 \u03bcg\/mL) on TH induction and on the number of total recovered spermatozoa (NTRS) were also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Significant differences were observed among the analyzed conditions (Kruskal\u2013Wallis test with Conover\n                      <jats:italic>post-hoc<\/jats:italic>\n                      analysis,\n                      <jats:italic>p<\/jats:italic>\n                      &amp;lt;\u00a00.001). Fertile men showed no significant difference between THE and THO, whereas significant differences were observed in men consulting for infertility. THE was significantly higher in fertile men than in men with infertility [median (interquartile range, IQR): 8.2% (4.9\u201314.8) vs. 4.9% (2.7\u20139.0);\n                      <jats:italic>p<\/jats:italic>\n                      &amp;lt;\u00a00.001]. THE values above the upper quartile of patients with infertility (&amp;gt; 9%) correlated with NTRS (Spearman\u2019s\n                      <jats:italic>rs<\/jats:italic>\n                      =\u00a00.779,\n                      <jats:italic>p<\/jats:italic>\n                      =\u00a00.0004). In paired samples, T4 treatment significantly increased NTRS [median (IQR): 4.4 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      (3.6\u20139.2) vs. 10.7 \u00d7 10\n                      <jats:sup>6<\/jats:sup>\n                      (6.1\u201316.7);\n                      <jats:italic>p<\/jats:italic>\n                      =\u00a00.00075], enabling samples to reach the threshold for intrauterine insemination.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Transition hyperactivated motility represents a novel and objective functional biomarker of sperm kinematic competence beyond conventional semen parameters. Measurement of THE in 10-\u03bcm chambers provides a robust analytical approach for functional sperm assessment. Combined with T4 induction, this strategy may offer clinically relevant information on sperm functional status. Prospective studies including pregnancy outcomes are needed to determine its predictive value for clinical fertility and assisted reproduction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fendo.2026.1870772","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T05:35:05Z","timestamp":1789968905000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Objective assessment of transition hyperactivated motility in 10-\u03bcm chambers: a functional predictor of sperm recovery in assisted reproduction"],"prefix":"10.3389","volume":"17","author":[{"given":"Gabriela Ruth","family":"Mendeluk","sequence":"first","affiliation":[{"name":"Laboratory of Male Fertility, Buenos Aires University Clinical Hospital \u201cJos\u00e9 de San Mart\u00edn\u201d, Instituto de Fisiopatolog\u00eda y Bioqu\u00edmica Cl\u00ednica (INFIBIOC), Faculty of Pharmacy and Biochemistry, University of Buenos 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