{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T23:07:18Z","timestamp":1754262438704,"version":"3.41.2"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI137485"],"award-info":[{"award-number":["R21AI137485"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Fungal Biol."],"abstract":"<jats:p><jats:italic>Aspergillus fumigatus<\/jats:italic> is a potentially deadly opportunistic human pathogen. <jats:italic>A. fumigatus<\/jats:italic> has evolved a variety of mechanisms to evade detection by the immune system. For example, the conidium surface is covered in a layer of 1,8-dihydroxynaphthalene (DHN) melanin which masks the antigen macrophages use for recognition. DHN melanin also protects conidia from ultraviolet radiation and gives <jats:italic>A. fumigatus<\/jats:italic> conidia their characteristic green-grayish color. Here, we conducted genomic analysis of two closely related white-spore natural variants of <jats:italic>A. fumigatus<\/jats:italic> in comparison to two closely related green-spore isolates to identify a genetic basis of the white-spore phenotype. Illumina whole-genome resequencing data of the four isolates was used to identify variants that were shared in the white-spore isolates and different from both the green-spore isolates and the Af293 reference genome (which is also a green-spore isolate). We identified 4,279 single nucleotide variants and 1,785 insertion\/deletions fitting this pattern. Among these, we identified 64 variants predicted to be high impact, loss-of-function mutations. One of these variants is a single nucleotide deletion that results in a frameshift in <jats:italic>pksP<\/jats:italic> (<jats:italic>Afu2g17600<\/jats:italic>), the core biosynthetic gene in the DHN melanin encoding gene cluster. The frameshift mutation in the white-spore isolates leads to a truncated protein in which a phosphopantetheine attachment site (PP-binding domain) is interrupted and an additional PP-binding domain and a thioesterase domain are omitted. Growth rate analysis of white-spore and green-spore isolates at 37\u00b0C and 48\u00b0C revealed that white-spore isolates are thermosensitive. Growth rate of <jats:italic>A. fumigatus<\/jats:italic> Af293 and a <jats:italic>pksP<\/jats:italic> null mutant in the Af293 background suggests <jats:italic>pksP<\/jats:italic> is not directly involved in the thermosensitivity phenotype. Further, our study identified a mutation in a gene (<jats:italic>Afu4g04740)<\/jats:italic> associated with thermal sensitivity in yeasts which could also be responsible for the thermosensitivity of the white-spore mutants. Overall, we used comparative genomics to identify the mutation and protein alterations responsible for the white-spore phenotype of environmental isolates of <jats:italic>A. fumigatus<\/jats:italic>.<\/jats:p>","DOI":"10.3389\/ffunb.2022.897954","type":"journal-article","created":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T13:29:50Z","timestamp":1654608590000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparative Genomics Reveals a Single Nucleotide Deletion in pksP That Results in White-Spore Phenotype in Natural Variants of Aspergillus fumigatus"],"prefix":"10.3389","volume":"3","author":[{"given":"John G.","family":"Gibbons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"D\u2019Avino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace W.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C.","family":"Rinker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jarrod R.","family":"Fortwendel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Paul","family":"Latge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00446-17","article-title":"And Universal System for Gene Manipulation in Aspergillus Fumigatus: In Vitro-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled With Microhomology Repair Templates","volume":"2","author":"Al Abdallah","year":"2017","journal-title":"mSphere"},{"key":"B2","doi-asserted-by":"publisher","first-page":"742","DOI":"10.1099\/mic.0.072520-0","article-title":"Genetic and Virulence Variation in an Environmental Population of the Opportunistic Pathogen Aspergillus Fumigatus","volume":"160","author":"Alshareef","year":"2014","journal-title":"Microbiol-Sgm"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.1128\/AAC.48.4.1197-1203.2004","article-title":"Aspergillus Fumigatus Variant With Decreased Susceptibility to Multiple Antifungals","volume":"48","author":"Balajee","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"B4","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"39","DOI":"10.3390\/jof4010039","article-title":"FungiDB: An Integrated Bioinformatic Resource for Fungi and Oomycetes","volume":"4","author":"Basenko","year":"2018","journal-title":"J. Fungi (Basel) 4"},{"key":"B6","doi-asserted-by":"publisher","first-page":"310","DOI":"10.3109\/13693780903148346","article-title":"Interstrain Variability in the Virulence of Aspergillus Fumigatus and Aspergillus Terreus in a Toll-Deficient Drosophila Fly Model of Invasive Aspergillosis","volume":"48","author":"Ben-Ami","year":"2010","journal-title":"Med. Mycol"},{"key":"B7","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1111\/j.1525-142X.2012.00535.x","article-title":"Evolution of Albinism in Cave Planthoppers by a Convergent Defect in the First Step of Melanin Biosynthesis","volume":"14","author":"Bilandzija","year":"2012","journal-title":"Evol. Dev."},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03415-19","article-title":"Contributions of Spore Secondary Metabolites to UV-C Protection and Virulence Vary in Different Aspergillus Fumigatus Strains","volume":"11","author":"Blachowicz","year":"2020","journal-title":"Mbio"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1186\/1471-2164-15-1011","article-title":"Illumina Identification of RsrA, a Conserved C2H2 Transcription Factor Coordinating the NapA Mediated Oxidative Stress Signaling Pathway in Aspergillus","volume":"15","author":"Bok","year":"2014","journal-title":"BMC Genomics"},{"key":"B10","doi-asserted-by":"publisher","first-page":"165rv13","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden Killers: Human Fungal Infections","volume":"4","author":"Brown","year":"2012","journal-title":"Sci. Transl. Med."},{"key":"B11","doi-asserted-by":"publisher","first-page":"843","DOI":"10.2307\/2261448","article-title":"A Survey of Ultraviolet-B Radiation in Forests","volume":"82","author":"Brown","year":"1994","journal-title":"J. Ecol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10682-020-10084-8","article-title":"When Animal Coloration is a Poor Match","volume":"35","author":"Caro","year":"2021","journal-title":"Evol. Ecol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"80","DOI":"10.4161\/fly.19695","article-title":"A Program for Annotating and Predicting the Effects of Single Nucleotide Polymorphisms, SnpEff: SNPs in the Genome of Drosophila Melanogaster Strain W(1118); Iso-2; Iso-3","volume":"6","author":"Cingolani","year":"2012","journal-title":"Fly"},{"key":"B14","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1111\/j.1365-2958.2006.05374.x","article-title":"The Nuclear GTPase Gsp1p can Affect Proper Telomeric Function Through the Sir4 Protein in Saccharomyces Cerevisiae","volume":"62","author":"Clement","year":"2006","journal-title":"Mol. Microbiol."},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01827","article-title":"The Genome of a Thermo Tolerant, Pathogenic Albino Aspergillus Fumigatus","volume":"9","author":"Couger","year":"2018","journal-title":"Front. Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/S0926-6593(66)80120-0","article-title":"The Induction and Repression of Nitrate Reductase in the Fungus Aspergillus Nidulans","volume":"113","author":"Cove","year":"1966","journal-title":"Biochim. Biophys. Acta"},{"key":"B17","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1038\/s41594-021-00650-1","article-title":"AlphaFold2 and the Future of Structural Biology","volume":"28","author":"Cramer","year":"2021","journal-title":"Nat. Struct. Mol. Biol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1083\/jcb.200509061","article-title":"The Mobile Nucleoporin Nup2p and Chromatin-Bound Prp20p Function in Endogenous NPC-Mediated Transcriptional Control","volume":"171","author":"Dilworth","year":"2005","journal-title":"J. Cell Biol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"D427","DOI":"10.1093\/nar\/gky995","article-title":"The Pfam Protein Families Database in 2019","volume":"47","author":"El-Gebali","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B20","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1007\/BF00273932","article-title":"Analysis of Yeast Prp20 Mutations and Functional Complementation by the Human Homolog Rcc1, a Protein Involved in the Control of Chromosome Condensation","volume":"227","author":"Fleischmann","year":"1991","journal-title":"Mol. Gen. Genet."},{"key":"B21","doi-asserted-by":"publisher","first-page":"44613","DOI":"10.1074\/jbc.M406758200","article-title":"Hydrolytic Polyketide Shortening by Ayg1p, a Novel Enzyme Involved in Fungal Melanin Biosynthesis","volume":"279","author":"Fujii","year":"2004","journal-title":"J. Biol. Chem."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.48550\/arXiv.1207.3907","article-title":"Haplotype-Based Variant Detection From Short-Read Sequencing","author":"Garrison","year":"2012","journal-title":"arXiv"},{"key":"B23","first-page":"2","article-title":"BioEdit: An Important Software for Molecular Biology","volume":"2","author":"Hall","year":"2011","journal-title":"GERF Bull. Biosci."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00440","article-title":"Aspergillus Fumigatus Melanins: Interference With the Host Endocytosis Pathway and Impact on Virulence","volume":"3","author":"Heinekamp","year":"2012","journal-title":"Front. Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1146\/annurev.anthro.33.070203.143955","article-title":"The Evolution of Human Skin and Skin Color","volume":"33","author":"Jablonski","year":"2004","journal-title":"Annu. Rev. Anthropol"},{"key":"B26","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly Accurate Protein Structure Prediction With AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"B27","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0252948","article-title":"Variation of Virulence of Five Aspergillus Fumigatus Isolates in Four Different Infection Models","volume":"16","author":"Keizer","year":"2021","journal-title":"PloS One"},{"key":"B29","doi-asserted-by":"publisher","first-page":"6246","DOI":"10.1073\/pnas.0913531107","article-title":"Structure and Function of an Iterative Polyketide Synthase Thioesterase Domain Catalyzing Claisen Cyclization in Aflatoxin Biosynthesis","volume":"107","author":"Korman","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01515-16","article-title":"Heterogeneity Among Isolates Reveals That Fitness in Low Oxygen Correlates With Aspergillus Fumigatus Virulence","volume":"7","author":"Kowalski","year":"2016","journal-title":"Mbio"},{"key":"B31","doi-asserted-by":"publisher","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"Molecular Evolutionary Genetics Analysis Across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1128\/CMR.12.2.310","article-title":"Aspergillus Fumigatus and Aspergillosis","volume":"12","author":"Latge","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"B33","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1146\/annurev-micro-030117-020406","article-title":"The Cell Wall of the Human Fungal Pathogen Aspergillus Fumigatus: Biosynthesis, Organization, Immune Response, and Virulence","volume":"71","author":"Latge","year":"2017","journal-title":"Annu. Rev. Microbiol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and Accurate Short Read Alignment With Burrows-Wheeler Transform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"B35","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"The Sequence Alignment\/Map Format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"B36","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.1101\/gr.205682.116","article-title":"Gene Discovery by Chemical Mutagenesis and Whole-Genome Sequencing in Dictyostelium","volume":"26","author":"Li","year":"2016","journal-title":"Genome Res."},{"key":"B37","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1038\/nrg3937","article-title":"Elucidating the Molecular Architecture of Adaptation via Evolve and Resequence Experiments","volume":"16","author":"Long","year":"2015","journal-title":"Nat. Rev. Genet."},{"key":"B38","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1111\/j.1462-5822.2006.00796.x","article-title":"Phagocytosis of Aspergillus Fumigatus Conidia by Murine Macrophages Involves Recognition by the Dectin-1 Beta-Glucan Receptor and Toll-Like Receptor 2","volume":"9","author":"Luther","year":"2007","journal-title":"Cell Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt Removes Adapter Sequences From High-Throughput Sequencing Reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"B40","doi-asserted-by":"publisher","first-page":"13775","DOI":"10.1074\/jbc.M110.169953","article-title":"Nuclear Protein Quality Is Regulated by the Ubiquitin-Proteasome System Through the Activity of Ubc4 and San1 in Fission Yeast","volume":"286","author":"Matsuo","year":"2011","journal-title":"J. Biol. Chem."},{"key":"B41","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1186\/1471-2105-7-339","article-title":"Tools for Integrated Sequence-Structure Analysis With UCSF Chimera","volume":"7","author":"Meng","year":"2006","journal-title":"BMC Bioinf."},{"key":"B42","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1099\/00222615-45-3-186","article-title":"Variation in Virulence of Aspergillus Fumigatus Strains in a Murine Model of Invasive Pulmonary Aspergillosis","volume":"45","author":"Mondon","year":"1996","journal-title":"J. Med. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1038\/nature04332","article-title":"Genomic Sequence of the Pathogenic and Allergenic Filamentous Fungus Aspergillus Fumigatus","volume":"438","author":"Nierman","year":"2005","journal-title":"Nature"},{"key":"B44","doi-asserted-by":"publisher","first-page":"3635","DOI":"10.1093\/bioinformatics\/btx445","article-title":"Gvolante for Standardizing Completeness Assessment of Genome and Transcriptome Assemblies","volume":"33","author":"Nishimura","year":"2017","journal-title":"Bioinformatics"},{"key":"B45","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1186\/1471-2180-9-177","article-title":"Melanin Is an Essential Component for the Integrity of the Cell Wall of Aspergillus Fumigatus Conidia","volume":"9","author":"Pihet","year":"2009","journal-title":"BMC Microbiol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1038\/ng1700","article-title":"Genetic Analysis of Cavefish Reveals Molecular Convergence in the Evolution of Albinism","volume":"38","author":"Protas","year":"2006","journal-title":"Nat. Genet."},{"key":"B47","doi-asserted-by":"publisher","first-page":"5702","DOI":"10.1073\/pnas.1013850108","article-title":"Genetic Basis of Eye and Pigment Loss in the Cave Crustacean, Asellus Aquaticus","volume":"108","author":"Protas","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"B48","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1093\/bioinformatics\/btq033","article-title":"BEDTools: A Flexible Suite of Utilities for Comparing Genomic Features","volume":"26","author":"Quinlan","year":"2010","journal-title":"Bioinformatics"},{"key":"B49","doi-asserted-by":"publisher","first-page":"203","DOI":"10.3314\/jjmm.43.203","article-title":"A New Experimental Murine Aspergillosis Model to Identify Strains of Aspergillus Fumigatus With Reduced Virulence","volume":"43","author":"Sarfati","year":"2002","journal-title":"Nihon Ishinkin Gakkai Zasshi"},{"key":"B50","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1093\/bioinformatics\/btv351","article-title":"BUSCO: Assessing Genome Assembly and Annotation Completeness With Single-Copy Orthologs","volume":"31","author":"Simao","year":"2015","journal-title":"Bioinformatics"},{"key":"B51","doi-asserted-by":"publisher","first-page":"D675","DOI":"10.1093\/nar\/gkr918","article-title":"FungiDB: An Integrated Functional Genomics Database for Fungi","volume":"40","author":"Stajich","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B52","doi-asserted-by":"publisher","first-page":"ii215","DOI":"10.1093\/bioinformatics\/btg1080","article-title":"Gene Prediction With a Hidden Markov Model and a New Intron Submodel","author":"Stanke","year":"2003","journal-title":"Bioinformatics"},{"key":"B53","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/j.jinf.2012.08.003","article-title":"Clinical Epidemiology of 960 Patients With Invasive Aspergillosis From the PATH Alliance Registry","volume":"65","author":"Steinbach","year":"2012","journal-title":"J. Infect."},{"key":"B54","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1007\/s00203-006-0144-2","article-title":"Characterisation of the Laccase-Encoding Gene Abr2 of the Dihydroxynaphthalene-Like Melanin Gene Cluster of Aspergillus Fumigatus","volume":"186","author":"Sugareva","year":"2006","journal-title":"Arch. Microbiol."},{"key":"B55","doi-asserted-by":"publisher","first-page":"a019786","DOI":"10.1101\/cshperspect.a019786","article-title":"Aspergillus Fumigatus and Related Species","volume":"5","author":"Sugui","year":"2014","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"B56","doi-asserted-by":"publisher","first-page":"3031","DOI":"10.1128\/JB.180.12.3031-3038.1998","article-title":"The Developmentally Regulated Alb1 Gene of Aspergillus Fumigatus: Its Role in Modulation of Conidial Morphology and Virulence","volume":"180","author":"Tsai","year":"1998","journal-title":"J. Bacteriol"},{"key":"B57","doi-asserted-by":"publisher","first-page":"29292","DOI":"10.1074\/jbc.M101998200","article-title":"Pentaketide Melanin Biosynthesis in Aspergillus Fumigatus Requires Chain-Length Shortening of a Heptaketide Precursor","volume":"276","author":"Tsai","year":"2001","journal-title":"J. Biol. Chem."},{"key":"B58","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1016\/j.chembiol.2012.10.002","article-title":"Characterization of a Fungal Thioesterase Having Claisen Cyclase and Deacetylase Activities in Melanin Biosynthesis","volume":"19","author":"Vagstad","year":"2012","journal-title":"Chem. Biol."},{"key":"B59","doi-asserted-by":"publisher","first-page":"11 10 1","DOI":"10.1002\/0471250953.bi1110s43","article-title":"From FastQ Data to High Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline","volume":"43","author":"Van der Auwera","year":"2013","journal-title":"Curr. Protoc. Bioinf."},{"key":"B60","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1038\/nrmicro.2017.90","article-title":"Aspergillus Fumigatus Morphology and Dynamic Host Interactions","volume":"15","author":"van de Veerdonk","year":"2017","journal-title":"Nat. Rev. Microbiol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"1206","DOI":"10.1101\/gad.3.8.1206","article-title":"Isolation and Characterization of Pre-Messenger Rna Splicing Mutants of Saccharomyces-Cerevisiae","volume":"3","author":"Vijayraghavan","year":"1989","journal-title":"Gene Dev."},{"key":"B62","doi-asserted-by":"publisher","first-page":"103601","DOI":"10.1016\/j.fgb.2021.103601","article-title":"Analysis of Genomic Characteristics and Their Influence on Metabolism in Aspergillus Luchuensis Albino Mutants Using Genome Sequencing","volume":"155","author":"Yamamoto","year":"2021","journal-title":"Fungal Genet. Biol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"20170635","DOI":"10.1098\/rspb.2017.0635","article-title":"Evolution of Cross-Resistance to Medical Triazoles in Aspergillus Fumigatus Through Selection Pressure of Environmental Fungicides","volume":"284","author":"Zhang","year":"2017","journal-title":"Proc. Biol. Sci."},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.3389\/ffunb.2020.617338","article-title":"Genome-Wide Association for Itraconazole Sensitivity in Non-Resistant Clinical Isolates of Aspergillus Fumigatus","volume":"1","author":"Zhao","year":"2021","journal-title":"Front. Fungal Biol."},{"key":"B65","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.01328-19","article-title":"Genome Sequences of Two Strains of the Food Spoilage Mold Aspergillus Fischeri","volume":"8","author":"Zhao","year":"2019","journal-title":"Microbiol. Resour. Announc."}],"container-title":["Frontiers in Fungal Biology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/ffunb.2022.897954\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T13:29:57Z","timestamp":1654608597000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/ffunb.2022.897954\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,7]]},"references-count":65,"alternative-id":["10.3389\/ffunb.2022.897954"],"URL":"https:\/\/doi.org\/10.3389\/ffunb.2022.897954","relation":{},"ISSN":["2673-6128"],"issn-type":[{"type":"electronic","value":"2673-6128"}],"subject":[],"published":{"date-parts":[[2022,6,7]]},"article-number":"897954"}}