{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:32:11Z","timestamp":1787725931285,"version":"build-2784847793"},"reference-count":73,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Fungal Biol."],"abstract":"<jats:p>\n                    Conidial germination requires rapid mobilization and reorganization of storage carbohydrates, yet the network architecture underlying this process remains poorly defined in filamentous fungi. Using quantitative GC\u2013MS\/MS profiling, we provide the first quantitative identification of major soluble sugar species across four germination stages of\n                    <jats:italic>Trichoderma asperelloides<\/jats:italic>\n                    T203 and compared them with four representative models in the Sordariomycetes (\n                    <jats:italic>Metarhizium anisopliae<\/jats:italic>\n                    ,\n                    <jats:italic>Cordyceps militaris<\/jats:italic>\n                    ,\n                    <jats:italic>Fusarium graminearum<\/jats:italic>\n                    , and\n                    <jats:italic>Neurospora crassa<\/jats:italic>\n                    ). In\n                    <jats:italic>T. asperelloides<\/jats:italic>\n                    , mannitol was the most prevalent measured sugar in dormant conidia, declined sharply at polarity establishment, and partially recovered at later stages, while trehalose displayed a reciprocal increase and other sugars remained comparatively stable. Comparative analyses revealed distinct species-specific carbon storage strategies: Dormant conidia of\n                    <jats:italic>T. asperelloides<\/jats:italic>\n                    ,\n                    <jats:italic>M. anisoplia<\/jats:italic>\n                    e, and\n                    <jats:italic>C. militaris<\/jats:italic>\n                    were mannitol-enriched, whereas in\n                    <jats:italic>N. crassa<\/jats:italic>\n                    and\n                    <jats:italic>F. graminearum<\/jats:italic>\n                    glucose was the most abundant; after germination onset, most species shifted toward glucose accumulation, but\n                    <jats:italic>T. asperelloides<\/jats:italic>\n                    uniquely transitioned from mannitol to trehalose dominance before partial re-accumulation of mannitol. Integration of sugar profiles with time-resolved RNA-seq and Bayesian network inference revealed conserved core interactions but also lineage-specific divergences in mannitol\/trehalose-associated central-carbon modules that correspond to distinct nutrient and lifestyle strategies during early colonization. A focused analysis in\n                    <jats:italic>T. asperelloides<\/jats:italic>\n                    uncovered extensive stage-dependent transcriptional remodeling of metabolic-process genes and a mannitol-centered module involving\n                    <jats:italic>mpd1<\/jats:italic>\n                    and\n                    <jats:italic>mtd1<\/jats:italic>\n                    (encoding mannitol\u20101\u2010phosphate 5\u2010dehydrogenase and mannitol dehydrogenase, respectively). Antisense-based knockdown of\n                    <jats:italic>mpd1<\/jats:italic>\n                    strongly reduced its transcript levels and led to stage-dependent upregulation of\n                    <jats:italic>mtd1<\/jats:italic>\n                    . However, these changes left mannitol content, soluble-sugar profiles, germination dynamics, and growth on mannitol essentially unchanged. Together, our comparative metabolic-network analysis shows that conidial mannitol and trehalose metabolism in\n                    <jats:italic>T. asperelloides<\/jats:italic>\n                    is embedded in a flexible, partially redundant central-carbon framework, and establishes this species as a tractable model for systems-level dissection of sugar metabolic regulation during early fungal development and colonization.\n                  <\/jats:p>","DOI":"10.3389\/ffunb.2026.1930613","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:04:39Z","timestamp":1787724279000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Conserved storage-carbohydrate metabolic modules are rewired during germination of Trichoderma asperelloides and other Sordariomycetes"],"prefix":"10.3389","volume":"7","author":[{"given":"Bar","family":"Tenennbaum","sequence":"first","affiliation":[{"name":"Department of Plant Pathology and Microbiology, The Robert H. 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Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem","place":["Rehovot, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Yale School of Public Health","place":["New Haven, CT, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey Peter","family":"Townsend","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Yale School of Public Health","place":["New Haven, CT, United States"]},{"name":"Department of Ecology and Evolutionary Biology, Yale University","place":["New Haven, CT, United States"]},{"name":"Program in Microbiology, Yale University","place":["New Haven, CT, United States"]},{"name":"Program in Computational Biology and Biomedical Informatics, Yale University","place":["New Haven, CT, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oded","family":"Yarden","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology, The Robert H. 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