{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T15:16:34Z","timestamp":1793718994706,"version":"4.6.1"},"reference-count":140,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genome Ed."],"abstract":"<jats:p>\n                    Genome editing using the CRISPR\/Cas system has revolutionized the field of genetic engineering, offering unprecedented opportunities for therapeutic applications\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Despite the numerous ongoing clinical trials focusing on\n                    <jats:italic>ex vivo<\/jats:italic>\n                    genome editing, recent studies emphasize the therapeutic promise of\n                    <jats:italic>in vivo<\/jats:italic>\n                    gene editing using CRISPR\/Cas technology. However, it is worth noting that the complete attainment of the inherent capabilities of\n                    <jats:italic>in vivo<\/jats:italic>\n                    therapy in humans is yet to be accomplished. Before the full realization of\n                    <jats:italic>in vivo<\/jats:italic>\n                    therapeutic potential, it is crucial to achieve enhanced specificity in selectively targeting defective cells while minimizing harm to healthy cells. This review examines emerging studies, focusing on CRISPR\/Cas-based pre-clinical and clinical trials for innovative therapeutic approaches for a wide range of diseases. Furthermore, we emphasize targeting cancer-specific sequences target in genes associated with tumors, shedding light on the diverse strategies employed in cancer treatment. We highlight the various challenges associated with\n                    <jats:italic>in vivo<\/jats:italic>\n                    CRISPR\/Cas-based cancer therapy and explore their prospective clinical translatability and the strategies employed to overcome these obstacles.\n                  <\/jats:p>","DOI":"10.3389\/fgeed.2024.1342193","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T23:29:28Z","timestamp":1706743768000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":42,"title":["Revolutionizing in vivo therapy with CRISPR\/Cas genome editing: breakthroughs, opportunities and challenges"],"prefix":"10.3389","volume":"6","author":[{"given":"Arturo","family":"Macarr\u00f3n Palacios","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Korus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bodo G. 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