{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T06:04:55Z","timestamp":1780985095123,"version":"3.54.1"},"reference-count":29,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genome Ed."],"abstract":"<jats:p>\n                    Myostatin (\n                    <jats:italic>MSTN<\/jats:italic>\n                    ) gene knockout has attracted considerable interest for enhancing meat production in livestock species due to its well-established role as a negative regulator of skeletal muscle growth. In this study, we report the clinical outcome of a bi-allelic\n                    <jats:italic>MSTN<\/jats:italic>\n                    knockout buffalo produced via somatic cell nuclear transfer (SCNT) that failed to survive beyond 100\u00a0days of age. The\n                    <jats:italic>MSTN<\/jats:italic>\n                    knockout calf exhibited a double-muscling phenotype, which was validated by changes in mRNA expression of transcripts associated with increased muscle mass and reduced subcutaneous fat. In addition, the calf had a telomere length similar to that of age-matched calves produced through artificial insemination. However, serum biochemistry analysis revealed decreased levels of globulin, GGTP, ALP, and bilirubin, alongside increased concentrations of AST, urea, blood urea nitrogen, uric acid, phosphorus, and potassium. Cytokine profiling (including IFN-\u03b3, IL-1\u03b1, IL-1\u03b2, IL-4, IL-6, IL-8, IL-10, IL-17\u03b1, IL-36R\u03b1, TNF-\u03b1, MIP-1\u03b1, MIP-1\u03b2, MCP-1, IP-10, and VEGF-\u03b1) demonstrated dysregulation of several inflammatory and chemotactic mediators. This case report highlights potential health and welfare challenges associated with producing\n                    <jats:italic>MSTN<\/jats:italic>\n                    knockout buffalo through cloning-based approaches.\n                  <\/jats:p>","DOI":"10.3389\/fgeed.2026.1822169","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T05:45:21Z","timestamp":1780983921000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical outcome of an SCNT-derived MSTN knockout buffalo: a case study"],"prefix":"10.3389","volume":"8","author":[{"given":"Priyanka","family":"Singh","sequence":"first","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kartikey","family":"Patel","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shavi","family":"Verma","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kusum","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjeet","family":"Verma","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babu Lal","family":"Jangir","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS)","place":["Hisar, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoj Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh L.","family":"Selokar","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division (ABTD), ICAR-National Dairy Research Institute (NDRI)","place":["Karnal, Haryana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1111\/age.12696","article-title":"The myostatin gene: an overview of mechanisms of action and its relevance to livestock animals","volume":"49","author":"Aiello","year":"2018","journal-title":"Anim. 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