{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:46:31Z","timestamp":1790052391242,"version":"4.0.1"},"reference-count":70,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genome Ed."],"abstract":"<jats:p>\n                    Sugarbeet (\n                    <jats:italic>Beta vulgaris<\/jats:italic>\n                    ssp.\n                    <jats:italic>vulgaris<\/jats:italic>\n                    , L.), is a vital temperate crop, supplying nearly 40% of the world\u2019s sugar. However, its high susceptibility to bacterial, fungal, and viral diseases creates an urgent need for improved, disease-resistant cultivars. The CRISPR\/Cas9 system has rapidly advanced plant genetic engineering by enabling precise and targeted genome modifications. Our goal is to establish a gene-editing platform in sugarbeet to support future development of disease-resistant lines by targeting the candidate genes. In this study, we applied CRISPR\/Cas9 to generate targeted mutations in two genes involved in chlorophyll biosynthesis and carotenoid-mediated leaf pigmentation:\n                    <jats:italic>magnesium chelatase<\/jats:italic>\n                    (Mg-chelatase) and\n                    <jats:italic>phytoene desaturase<\/jats:italic>\n                    (PDS). Two CRISPR\/Cas9 constructs, each carrying an sgRNA targeting either Mg-chelatase or PDS, were developed and mobilized into\n                    <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\n                    . A total of 233 and 200 hypocotyl explants were transformed with constructs targeting Mg-chelatase and PDS, resulting in regeneration efficiencies of 8% and 14% on kanamycin selection medium, respectively. Light green, yellow, variegated yellow-green, and albino phenotypes were observed among the putative transformants, whereas non-edited transformed lines resembled untransformed control plants. Targeted mutations, including insertions, deletions, and substitutions of nucleotides, were identified at both genomic loci, with editing efficiencies of 60.0% for Mg-chelatase and 68.75% for PDS underscoring the effectiveness of this approach in sugarbeet, a recalcitrant crop. Deletions ranged from 5 to 28 bp in Mg-chelatase and 2 to 21 bp in PDS, while insertion events consisted of single-base additions in Mg-chelatase edited lines and larger insertions of 7\u201316 bp in PDS mutants. The results demonstrate the successful deployment of CRISPR\/Cas9 for targeted genome engineering in sugarbeet and establish a reliable platform for future gene-editing efforts aimed at enhancing resistance to a wide range of pathogens and diseases affecting the crop.\n                  <\/jats:p>","DOI":"10.3389\/fgeed.2026.1875376","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:35:26Z","timestamp":1784612126000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["CRISPR\/Cas9-based gene-editing platform development by targeting genes encoding magnesium chelatase and phytoene desaturase in sugarbeet (Beta vulgaris L.)"],"prefix":"10.3389","volume":"8","author":[{"given":"Zainul A.","family":"Khan","sequence":"first","affiliation":[{"name":"The Oak Ridge Institute for Science and Education, United States Department of Agriculture, Agricultural Research Service","place":["Fargo, ND, United States"]},{"name":"United States Department of Agriculture, Agricultural Research Service, Sugarbeet Research Unit, Edward T. Schafer Agricultural Research Center","place":["Fargo, ND, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tinley","family":"Hathaway","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Sugarbeet Research Unit, Edward T. Schafer Agricultural Research Center","place":["Fargo, ND, United States"]},{"name":"Department of Plant Pathology, Microbiology and Biotechnology, North Dakota State University","place":["Fargo, ND, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenggen","family":"Chu","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Sugarbeet Research Unit, Edward T. Schafer Agricultural Research Center","place":["Fargo, ND, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeev","family":"Gupta","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Cereal Crops Improvement Research Unit, Edward T. Schafer Agricultural Research Center","place":["Fargo, ND, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melvin","family":"Bolton","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Sugarbeet Research Unit, Edward T. Schafer Agricultural Research Center","place":["Fargo, ND, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanitharani","family":"Ramachandran","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture, Agricultural Research Service, Sugarbeet Research Unit, Edward T. 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