{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T05:03:48Z","timestamp":1789967028809,"version":"4.0.1"},"reference-count":92,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genome Ed."],"abstract":"<jats:p>\n                    Soil salinity is a critical abiotic stress that severely constrains rice productivity and threatens global food security. Phospholipase D (PLD) enzymes play pivotal roles in plant stress signaling; however, the specific function of\n                    <jats:italic>OsPLD\u03b21<\/jats:italic>\n                    in rice salt stress responses remains unclear. We here investigated the role of\n                    <jats:italic>OsPLD\u03b21<\/jats:italic>\n                    using knockout mutants to elucidate its regulatory function under saline conditions.\n                    <jats:italic>Ospld\u03b21<\/jats:italic>\n                    -mutant lines exhibited substantially improved salt tolerance in contrast to wild-type plants. Improved tolerance was associated with elevated reactive oxygen species (ROS) scavenging capacity, indicating improved oxidative stress management. Furthermore, the mutant lines displayed increased expression of ion transport and homeostasis genes and maintained photosynthetic efficiency and agronomic performance. Collectively, these physiological and molecular responses demonstrate that\n                    <jats:italic>OsPLD\u03b21<\/jats:italic>\n                    negatively regulates salt stress tolerance in rice. This research highlights the role of\n                    <jats:italic>OsPLD\u03b21<\/jats:italic>\n                    in salt tolerance in rice and lays the groundwork for developing salinity-resistant crop varieties through genetic manipulation.\n                  <\/jats:p>","DOI":"10.3389\/fgeed.2026.1904040","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:28:28Z","timestamp":1789964908000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["CRISPR\/Cas9-mediated disruption of OsPLD\u03b21 enhances salt stress tolerance in rice through modulation of antioxidant defense"],"prefix":"10.3389","volume":"8","author":[{"given":"Sangeetha","family":"Karippadakam","sequence":"first","affiliation":[{"name":"Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology","place":["New Delhi, India"]},{"name":"School of Biotechnology, Amrita Vishwa Vidyapeetham","place":["Kollam, Kerala, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. Mohan M.","family":"Achary","sequence":"additional","affiliation":[{"name":"Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalia","family":"Vishnudasan","sequence":"additional","affiliation":[{"name":"School of Biotechnology, Amrita Vishwa Vidyapeetham","place":["Kollam, Kerala, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemangini","family":"Parmar","sequence":"additional","affiliation":[{"name":"Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Balamurugan","sequence":"additional","affiliation":[{"name":"ICAR- Indian Agricultural Research Institute (IARI), Plant Pathology","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallesham","family":"Bulle","sequence":"additional","affiliation":[{"name":"School of Plant, Environmental and Soil Science, Louisiana State University Agricultural Center","place":["Baton Rouge, LA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganesan","family":"Prakash","sequence":"additional","affiliation":[{"name":"ICAR- Indian Agricultural Research Institute (IARI), Plant Pathology","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malireddy K.","family":"Reddy","sequence":"additional","affiliation":[{"name":"Crop Improvement Group, International Centre for Genetic Engineering and Biotechnology","place":["New Delhi, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1002\/em.21719","article-title":"Aluminum induces oxidative burst, cell wall NADH peroxidase activity, and DNA damage in root cells of Allium cepa L","volume":"53","author":"Achary","year":"2012","journal-title":"Environ. 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