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Genet."],"abstract":"<jats:p>Previously, we demonstrated an integrated genomic convergence and network analysis approach to identify the candidate genes associated with the complex neurodegenerative disorder, Alzheimer\u2019s disease (AD). Here, we performed a pilot study to validate the <jats:italic>in silico<\/jats:italic> approach by studying the association of genetic variants from three identified critical genes, <jats:italic>APOE<\/jats:italic>, <jats:italic>EGFR<\/jats:italic>, and <jats:italic>ACTB<\/jats:italic>, with AD. A total of 103 patients with AD and 146 healthy controls were recruited. A total of 46 single-nucleotide polymorphisms (SNPs) spanning the three genes were genotyped, of which only 19 SNPs were included in the final analyses after excluding non-polymorphic and Hardy\u2013Weinberg equilibrium-violating SNPs. Apart from our previously reported <jats:italic>APOE<\/jats:italic> \u03b54, four other SNPs in <jats:italic>APOE<\/jats:italic> (rs405509, rs7259620, \u2212rs769449, and rs7256173), one in <jats:italic>EGFR<\/jats:italic> (rs6970262), and one in <jats:italic>ACTB<\/jats:italic> (rs852423) showed a significant association with AD (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Our results validate the reliability of genomic convergence and network analysis approach in identifying the AD-associated candidate genes.<\/jats:p>","DOI":"10.3389\/fgene.2021.722221","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T12:22:31Z","timestamp":1639052551000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["Validating a Genomic Convergence and Network Analysis Approach Using Association Analysis of Identified Candidate Genes in Alzheimer\u2019s Disease"],"prefix":"10.3389","volume":"12","author":[{"given":"Puneet","family":"Talwar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suman","family":"Kushwaha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chitra","family":"Rawat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harpreet","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankit","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachna","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Puneet","family":"Chandna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Tucci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciano","family":"Saso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritushree","family":"Kukreti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/s0014-5793(97)01543-3","article-title":"Allelic Polymorphisms in the Transcriptional Regulatory Region of Apolipoprotein E Gene","volume":"421","author":"Artiga","year":"1998","journal-title":"FEBS Lett."},{"key":"B2","doi-asserted-by":"publisher","first-page":"87ps24","DOI":"10.1126\/scitranslmed.3001628","article-title":"Breaking Free of Sample Size Dogma to Perform Innovative Translational Research","volume":"3","author":"Bacchetti","year":"2011","journal-title":"Sci. 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