{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T01:20:33Z","timestamp":1789089633719,"version":"build-2803163510"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T00:00:00Z","timestamp":1643068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>The selection of testis size can improve the reproductive capacity of livestock used for artificial insemination and has been considered as an important strategy for accelerating the breeding process. Although much work has been done to investigate the mechanisms of testis development in various species, there is little information available in regard to the differences in transcriptomic profiling of sheep testes at different developmental stages. In this work, we aimed to identify differentially expressed genes (DEGs) by RNA-Seq in sheep during different growth stages, including 0 month old (infant, M0), 3\u00a0months old (puberty, M3), 6\u00a0months old (sexual maturity, M6) and 12\u00a0months old (body maturity, M12). A total of 4,606 (2,381 up and 2,225 down), 7,500 (4,368 up and 3,132 down), 15 (8 up and seven down) DEGs were identified in M3_vs_M0, M6_vs_M3, and M12_vs_M6 comparison, respectively. Of which, a number of genes were continuously up-regulated and down-regulated with testicular development, including <jats:italic>ODF3<\/jats:italic>, <jats:italic>ZPBP1<\/jats:italic>, <jats:italic>PKDREJ<\/jats:italic>, <jats:italic>MYBL1<\/jats:italic>, <jats:italic>PDGFA<\/jats:italic>, <jats:italic>IGF1<\/jats:italic>, <jats:italic>LH<\/jats:italic>, <jats:italic>INSL3<\/jats:italic>, <jats:italic>VIM<\/jats:italic>, <jats:italic>AMH<\/jats:italic>, <jats:italic>INHBA<\/jats:italic>, <jats:italic>COL1A1<\/jats:italic>, <jats:italic>COL1A2<\/jats:italic>, and <jats:italic>INHA<\/jats:italic>. GO analysis illustrated that DEGs were mainly involved in testis development and spermatogenesis. KEGG analysis identified several important pathways and verified several reproduction-associated DEGs such as <jats:italic>COL1A1<\/jats:italic>, <jats:italic>COL1A2<\/jats:italic>, <jats:italic>PDGFA<\/jats:italic>, and <jats:italic>IGF1.<\/jats:italic> In addition, two gene modules highly associated with testis development and core genes with testis size were identified using weighted gene co-expression network analysis (WGCNA), including hub genes positively associated with testis size such as <jats:italic>RANBP9<\/jats:italic>, <jats:italic>DNAH17<\/jats:italic>, <jats:italic>SPATA4<\/jats:italic>, <jats:italic>CIB4<\/jats:italic> and <jats:italic>SPEM1<\/jats:italic>, and those negatively associated with testis size such as <jats:italic>CD81<\/jats:italic>, <jats:italic>CSK<\/jats:italic>, <jats:italic>PDGFA<\/jats:italic>, <jats:italic>VIM,<\/jats:italic> and <jats:italic>INHBA<\/jats:italic>. This study comprehensively identified key genes related to sheep testicular development, which may provide potential insights for understanding male fertility and better guide in animal breeding.<\/jats:p>","DOI":"10.3389\/fgene.2021.773695","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T08:27:22Z","timestamp":1643099242000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Identification of Key Genes Related to Postnatal Testicular Development Based on Transcriptomic Data of Testis in Hu Sheep"],"prefix":"10.3389","volume":"12","author":[{"given":"Haiyue","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengwei","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanhong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangpeng","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,25]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"138","DOI":"10.17533\/udea.rccp.v30n2a05","article-title":"Relationship between Body and Testicular Measurements in Young buffalo Bulls in Cuba","volume":"30","author":"Almaguer","year":"2017","journal-title":"RCCP"},{"key":"B2","doi-asserted-by":"publisher","first-page":"R106","DOI":"10.1186\/gb-2010-11-10-r106","article-title":"Differential Expression Analysis for Sequence Count Data","volume":"11","author":"Anders","year":"2010","journal-title":"Genome Biol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq--a Python Framework to Work with High-Throughput Sequencing Data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"B4","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1007\/s00114-017-1505-1","article-title":"Histological Analysis and Identification of Spermatogenesis-Related Genes in 2-, 6-, and 12-Month-Old Sheep Testes","volume":"104","author":"Bai","year":"2017","journal-title":"Sci. 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