{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:20:47Z","timestamp":1787494847205,"version":"build-2736575974"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,6]],"date-time":"2021-12-06T00:00:00Z","timestamp":1638748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>\n                    Dermatomyositis (DM), an inflammatory disorder, is often associated with interstitial lung disease (ILD). However, the underlying mechanism remains unclear. Our study performed RNA sequencing (RNA-seq) and integrative bioinformatics analysis of differentially expressed genes (DEGs) in patients with dermatomyositis-associated interstitial lung disease (DM-ILD) and healthy controls. A total of 2,018 DEGs were identified between DM-ILD and healthy blood samples. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that DEGs were mainly involved in immune- and inflammatory-related biological processes and pathways. Disease ontology (DO) enrichment analysis identified 35 candidate key genes involved in both skin and lung diseases. Meanwhile, a total of 886 differentially expressed alternative splicing (AS) events were found between DM-ILD and healthy blood samples. After overlapping DEGs with differential AS genes, the plasminogen activator and urokinase receptor (\n                    <jats:italic>PLAUR<\/jats:italic>\n                    ) involved in immune-related biological processes and complement and coagulation cascades was screened and identified as the most important gene associated with DM-ILD. The protein\u2013protein interaction (PPI) network revealed that\n                    <jats:italic>PLAUR<\/jats:italic>\n                    had interactions with multiple candidate key genes. Moreover, we observed that there were significantly more neutrophils and less naive B cells in DM-ILD samples than in healthy samples. And the expression of\n                    <jats:italic>PLAUR<\/jats:italic>\n                    was significantly positively correlated with the abundance of neutrophils. Significant higher abundance of\n                    <jats:italic>PLAUR<\/jats:italic>\n                    in DM-ILD patients than healthy controls was validated by RT-qPCR. In conclusion, we identified\n                    <jats:italic>PLAUR<\/jats:italic>\n                    as an important player in regulating DM-ILD by neutrophil-associated immune response. These findings enrich our understanding, which may benefit DM-ILD patients.\n                  <\/jats:p>","DOI":"10.3389\/fgene.2021.784215","type":"journal-article","created":{"date-parts":[[2021,12,6]],"date-time":"2021-12-06T01:56:35Z","timestamp":1638755795000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":15,"title":["Transcriptome Sequencing Identifies PLAUR as an Important Player in Patients With Dermatomyositis-Associated Interstitial Lung Disease"],"prefix":"10.3389","volume":"12","author":[{"given":"Juan","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyan","family":"Luan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,12,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1014","DOI":"10.2174\/1389201021666201001142416","article-title":"The Functional Impact of Alternative Splicing and Single Nucleotide Polymorphisms in Rheumatoid Arthritis","volume":"22","author":"Aravilli","year":"2021","journal-title":"Curr Pharm Biotechnol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1111\/bjd.17079","article-title":"Measurement of Cytokines and Chemokines and Association With Clinical Severity of Dermatomyositis and Clinically Amyopathic Dermatomyositis","volume":"179","author":"Chen","year":"2018","journal-title":"Br. 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