{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T03:33:14Z","timestamp":1768707194971,"version":"3.49.0"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T00:00:00Z","timestamp":1668643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>Cyclin A (<jats:italic>CycA<\/jats:italic>) plays essential roles in regulating multiple steps of the cell cycle, and it affects gonad development in mammals and invertebrates. Previous RNA interference (RNAi) analysis revealed that knocking-down the expression of <jats:italic>CycA<\/jats:italic> in female oriental river prawns (<jats:italic>Macrobrachium nipponense<\/jats:italic>) inhibited ovarian development. <jats:italic>CycA<\/jats:italic> was also predicted to have regulatory roles in reproductive development of male <jats:italic>M. nipponense<\/jats:italic> based on significant changes of <jats:italic>Mn-CycA<\/jats:italic> expression after eyestalk ablation. The goal of this study was to investigate the potential functions of <jats:italic>CycA<\/jats:italic> in the reproductive development of male <jats:italic>M. nipponense<\/jats:italic> using RNAi and histological observations. Quantitative real-time PCR analysis revealed that both single-side and double-side eyestalk ablation stimulated the expressions of <jats:italic>Mn-CycA<\/jats:italic>, and the expression was higher in prawns with double-side eyestalk ablation (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). <jats:italic>Mn-CycA<\/jats:italic> expression was significantly higher in the testis and androgenic gland during the reproductive season than during the non-reproductive season (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). In the RNAi analysis, <jats:italic>Mn-CycA<\/jats:italic> expression significantly decreased after prawns were injected with <jats:italic>dsCycA<\/jats:italic>, and the expression of <jats:italic>insulin-like androgenic gland hormone<\/jats:italic> (<jats:italic>Mn-IAG<\/jats:italic>) also decreased as <jats:italic>Mn-CycA<\/jats:italic> expression decreased. This result indicated that <jats:italic>CycA<\/jats:italic> positively regulated the expression of <jats:italic>IAG<\/jats:italic> in <jats:italic>M. nipponense<\/jats:italic>. Histological observations revealed that the number of sperm decreased dramatically to &amp;lt;5% of the total cells in the testis of the <jats:italic>dsCycA<\/jats:italic>-treated group compared to that of control group on day 14, indicating that knockdown of <jats:italic>Mn-CycA<\/jats:italic> expression inhibited testis development by affecting the expression of <jats:italic>Mn-IAG<\/jats:italic> in <jats:italic>M. nipponense<\/jats:italic>. These results highlighted the functions of <jats:italic>CycA<\/jats:italic> in male reproductive development of <jats:italic>M. nipponense<\/jats:italic>, which can be applied to future studies of male reproduction in other crustacean species.<\/jats:p>","DOI":"10.3389\/fgene.2022.1053826","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T07:37:02Z","timestamp":1668670622000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["RNA interference analysis of potential functions of cyclin A in the reproductive development of male oriental river prawns (Macrobrachium nipponense)"],"prefix":"10.3389","volume":"13","author":[{"given":"Wenyi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiwei","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengchao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianyong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sufei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongsheng","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shubo","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongtuo","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/j.aquaculture.2004.04.010","article-title":"Effects of captivity and eyestalk ablation on antioxidant status of shrimps (Farfantepenaeus paulensis)","volume":"238","author":"Almeida","year":"2004","journal-title":"Aquaculture"},{"key":"B2","doi-asserted-by":"publisher","first-page":"e0117859","DOI":"10.1371\/journal.pone.0117859","article-title":"Spatial reorganization of the endoplasmic reticulum during mitosis relies on mitotic kinase cyclin A in the early Drosophila embryo","volume":"10","author":"Bergman","year":"2015","journal-title":"PLoS ONE"},{"key":"B3","doi-asserted-by":"publisher","first-page":"3711","DOI":"10.1534\/g3.116.033050","article-title":"Distinct and overlapping requirements for cyclins A, B, and B3 in drosophila female meiosis","volume":"6","author":"Bourouh","year":"2016","journal-title":"G3"},{"key":"B4","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.applanim.2012.06.002","article-title":"Eyestalk ablation procedures tominimize pain in the freshwater prawn Macrobrachium Americanum","volume":"140","author":"Diarte-Plata","year":"2012","journal-title":"Appl. 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