{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T05:25:27Z","timestamp":1755926727867,"version":"3.41.2"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T00:00:00Z","timestamp":1672790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p><jats:bold>Objective:<\/jats:bold> To explore the functions of mRNAs and lncRNAs in the occurrence of uterine leiomyomas (ULs) and further clarify the pathogenesis of UL by detecting the differential expression of mRNAs and lncRNAs in 10 cases of UL tissues and surrounding normal myometrial tissues by high-throughput RNA sequencing.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The tissue samples of 10 patients who underwent hysterectomy for UL in Lianyungang Maternal and Child Health Hospital from January 2016 to December 2021 were collected. The differentially expressed mRNAs (DEmRNAs) and lncRNAs (DElncRNAs) were identified and further analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. The protein\u2013protein interaction network (PPI) was constructed in Cytoscape software. Functional annotation of the nearby target cis\u2010DEmRNAs of DElncRNAs was performed with the Database for Annotation, Visualization, and Integrated Discovery (DAVID) (<jats:ext-link>https:\/\/david.ncifcrf.gov\/<\/jats:ext-link>). Meanwhile, the co-expression network of DElncRNA\u2013DEmRNA was constructed in Cytoscape software.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> A total of 553 DElncRNAs (283 upregulated DElncRNAs and 270 downregulated DElncRNAs) and 3,293 DEmRNAs (1,632 upregulated DEmRNAs and 1,661 downregulated DEmRNAs) were obtained. GO pathway enrichment analysis revealed that several important pathways were significantly enriched in UL such as blood vessel development, regulation of ion transport, and external encapsulating structure organization. In addition, cytokine\u2013cytokine receptor interaction, neuroactive ligand\u2013receptor interaction, and complement and coagulation cascades were significantly enriched in KEGG pathway enrichment analysis. A total of 409 DElncRNAs\u2013nearby-targeted DEmRNA pairs were detected, which included 118 DElncRNAs and 136 DEmRNAs. Finally, we found that the top two DElncRNAs with the most nearby DEmRNAs were BISPR and AC012531.1.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> These results suggested that 3,293 DEmRNAs and 553 DElncRNAs were differentially expressed in UL tissue and normal myometrium tissue, which might be candidate-identified therapeutic and prognostic targets for UL and be considered as offering several possible mechanisms and pathogenesis of UL in the future.<\/jats:p>","DOI":"10.3389\/fgene.2022.1053845","type":"journal-article","created":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T06:42:36Z","timestamp":1672814556000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["An integrative analysis of an lncRNA\u2013mRNA competing endogenous RNA network to identify functional lncRNAs in uterine leiomyomas with RNA sequencing"],"prefix":"10.3389","volume":"13","author":[{"given":"Fanfei","family":"Meng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijing","family":"Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maofang","family":"Hua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1016\/j.fertnstert.2020.07.024","article-title":"Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis","volume":"115","author":"Chuang","year":"2021","journal-title":"Fertil. Steril."},{"key":"B2","doi-asserted-by":"publisher","first-page":"3513","DOI":"10.1007\/s11010-021-04174-6","article-title":"Wnt\/\u03b2-catenin signaling pathway in uterine leiomyoma: Role in tumor biology and targeting opportunities","volume":"476","author":"El","year":"2021","journal-title":"Mol. Cell. Biochem."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1007\/s43032-021-00571-w","article-title":"Emerging roles of long non-coding RNAs in uterine leiomyoma pathogenesis: A review","volume":"29","author":"Falahati","year":"2021","journal-title":"Reprod. Sci."},{"key":"B4","doi-asserted-by":"publisher","first-page":"E354","DOI":"10.1002\/lary.24700","article-title":"Overexpression of BST2 is associated with nodal metastasis and poorer prognosis in oral cavity cancer","volume":"124","author":"Fang","year":"2014","journal-title":"Laryngoscope"},{"key":"B5","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/ijgo.13102","article-title":"Epidemiology and management of uterine fibroids","volume":"149","author":"Giuliani","year":"2020","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"B6","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1186\/s12935-021-02404-x","article-title":"LncRNA HCP5 as a potential therapeutic target and prognostic biomarker for various cancers: A metaanalysis and bioinformatics analysis","volume":"21","author":"Hu","year":"2021","journal-title":"Cancer Cell Int."},{"key":"B7","doi-asserted-by":"publisher","first-page":"e1695","DOI":"10.1002\/wrna.1695","article-title":"Non-coding RNAs and their cross-talks impacting reproductive health of women","volume":"13","author":"Jain","year":"2022","journal-title":"Wiley Interdiscip. Rev. RNA"},{"key":"B8","doi-asserted-by":"publisher","first-page":"77","DOI":"10.3390\/ncrna7040077","article-title":"Common features in lncRNA annotation and classification: A survey","volume":"7","author":"Klapproth","year":"2021","journal-title":"Noncoding. RNA"},{"key":"B9","doi-asserted-by":"publisher","first-page":"166293","DOI":"10.1016\/j.bbadis.2021.166293","article-title":"Non-coding RNAs as new autophagy regulators in cancer progression","volume":"1868","author":"Lin","year":"2022","journal-title":"Biochim. Biophys. Acta. Mol. Basis Dis."},{"key":"B10","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.gde.2021.11.005","article-title":"Understanding lncRNA-protein assemblies with imaging and single-molecule approaches","volume":"72","author":"Liu","year":"2021","journal-title":"Curr. Opin. Genet. Dev."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1892","DOI":"10.1016\/j.fertnstert.2007.08.039","article-title":"Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics","volume":"90","author":"Lv","year":"2008","journal-title":"Fertil. Steril."},{"key":"B12","doi-asserted-by":"publisher","first-page":"4908","DOI":"10.1007\/s00261-021-03126-4","article-title":"Uterine leiomyomas revisited with review of literature","volume":"46","author":"Mathew","year":"2021","journal-title":"Abdom. Radiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1093\/intimm\/dxaa008","article-title":"Mechanisms of new blood-vessel formation and proliferative heterogeneity of endothelial cells","volume":"32","author":"Naito","year":"2020","journal-title":"Int. Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"112265","DOI":"10.1016\/j.biopha.2021.112265","article-title":"Gene regulation by antisense transcription: A focus on neurological and cancer diseases","volume":"145","author":"Najafi","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"B15","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/978-94-017-7417-8_9","article-title":"Non-coding RNAs in uterine development, function and disease","volume":"886","author":"Nothnick","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"2853","DOI":"10.21873\/anticanres.11637","article-title":"The expression of BTS-2 enhances cell growth and invasiveness in renal cell carcinoma","volume":"37","author":"Pham","year":"2017","journal-title":"Anticancer Res."},{"key":"B17","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1002\/term.3277","article-title":"The role of long Non-Coding RNAs and circular RNAs in bone regeneration: Modulating MiRNAs function","volume":"16","author":"Ping","year":"2021","journal-title":"J. Tissue Eng. Regen. Med."},{"key":"B18","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1055\/s-0037-1606569","article-title":"Uterine leiomyoma: New perspectives on an old disease","volume":"35","author":"Segars","year":"2017","journal-title":"Semin. Reprod. Med."},{"key":"B19","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1093\/humupd\/dmt042","article-title":"The role of angiogenic factors in fibroid pathogenesis: Potential implications for future therapy","volume":"20","author":"Tal","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"B20","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1097\/GME.0000000000001438","article-title":"Uterine fibroids in menopause and perimenopause","volume":"27","author":"Ulin","year":"2020","journal-title":"Menopause"},{"key":"B21","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.fertnstert.2005.07.1294","article-title":"Spatial differences in biologic activity of large uterine leiomyomata","volume":"85","author":"Wei","year":"2006","journal-title":"Fertil. Steril."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.1261\/rna.078971.121","article-title":"Long noncoding RNA and protein abundance in lncRNPs","volume":"27","author":"Wu","year":"2021","journal-title":"Rna"},{"key":"B23","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1186\/s12885-019-5775-1","article-title":"Lnc-AL445665.1-4 may be involved in the development of multiple uterine leiomyoma through interacting with miR-146b-5p","volume":"19","author":"Yang","year":"2019","journal-title":"Bmc Cancer"},{"key":"B24","doi-asserted-by":"publisher","first-page":"112013","DOI":"10.1016\/j.biopha.2021.112013","article-title":"Pathogenesis, diagnosis and treatment of uterine lipoleiomyoma: A review","volume":"142","author":"Yuan","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"B25","doi-asserted-by":"publisher","first-page":"2058738418772652","DOI":"10.1177\/2058738418772652","article-title":"LncRNA BISPR promotes the progression of thyroid papillary carcinoma by regulating miR-21-5p","volume":"32","author":"Zhang","year":"2018","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"13579","DOI":"10.3390\/ijms222413579","article-title":"Long noncoding RNAs: Recent insights into their role in male infertility and their potential as biomarkers and therapeutic targets","volume":"22","author":"Zhao","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"536346","DOI":"10.3389\/fonc.2021.536346","article-title":"LncRNA APTR promotes uterine leiomyoma cell proliferation by targeting ER\u03b1 to activate the wnt\/\u03b2-catenin pathway","volume":"11","author":"Zhou","year":"2021","journal-title":"Front. Oncol."}],"container-title":["Frontiers in Genetics"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fgene.2022.1053845\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T06:42:37Z","timestamp":1672814557000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fgene.2022.1053845\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,4]]},"references-count":27,"alternative-id":["10.3389\/fgene.2022.1053845"],"URL":"https:\/\/doi.org\/10.3389\/fgene.2022.1053845","relation":{},"ISSN":["1664-8021"],"issn-type":[{"type":"electronic","value":"1664-8021"}],"subject":[],"published":{"date-parts":[[2023,1,4]]},"article-number":"1053845"}}