{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T08:55:07Z","timestamp":1770540907574,"version":"3.49.0"},"reference-count":82,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>Unlike gonochoristic fishes, sex is fixed after gonadal differentiation (primary sex determination), and sex can be altered in adults (secondary sex determination) of hermaphroditic fish species. The secondary sex determination of hermaphroditic fish has focused on the differences between testicular tissue and ovarian tissue during the sex change process. However, comprehensive studies analyzing ovarian tissue or testicular tissue independently have not been performed. Hermaphroditic black porgy shows a digonic gonad (ovarian tissue with testicular tissue separated by connective tissue). Protandrous black porgy has stable maleness during the first two reproductive cycles (&amp;lt;2 years old), and approximately 50% enter femaleness (natural sex change) during the third reproductive cycle. Precocious femaleness is rarely observed in the estradiol-17\u03b2 (E<jats:sub>2<\/jats:sub>)-induced female phase (oocytes maintained at the primary oocyte stage), and a reversible female-to-male sex change is found after E<jats:sub>2<\/jats:sub> is withdrawn in &amp;lt;2-year-old fish. However, precocious femaleness (oocytes entering the vitellogenic oocyte stage) is observed in testis-removed fish in &amp;lt;2-year-old fish. We used this characteristic to study secondary sex determination (femaleness) in ovarian tissue <jats:italic>via<\/jats:italic> transcriptomic analysis. Cell proliferation analysis showed that BrdU (5-bromo-2\u2032-deoxyuridine)-incorporated germline cells were significantly increased in the testis-removed fish (female) compared to the control (sham) fish (male) during the nonspawning season (2\u00a0months after surgery). qPCR analysis showed that there were no differences in pituitary-releasing hormones (<jats:italic>lhb<\/jats:italic> and <jats:italic>gtha<\/jats:italic>) in pituitary and ovarian steroidogenesis-related factors (<jats:italic>star<\/jats:italic>, <jats:italic>cyp11a1<\/jats:italic>, <jats:italic>hsd3b1<\/jats:italic>, and <jats:italic>cyp19a1a<\/jats:italic>) or female-related genes (<jats:italic>wnt4a<\/jats:italic>, <jats:italic>bmp15<\/jats:italic>, <jats:italic>gdf9<\/jats:italic>, <jats:italic>figla<\/jats:italic>, and <jats:italic>foxl2<\/jats:italic>) in ovarian tissues between intact and testis-removed fish (2\u00a0months after surgery). Low expression of pituitary <jats:italic>fshb<\/jats:italic> and ovarian <jats:italic>cyp17a1<\/jats:italic> was found after 2\u00a0months of surgery. However, we did find small numbers of genes (289 genes) showing sexual fate dimorphic expression in both groups by transcriptomic analysis (1\u00a0month after surgery). The expression profiles of these differentially expressed genes were further examined by qPCR. Our present work identified several candidate genes in ovarian tissue that may be involved in the early period of secondary sex determination (femaleness) in black porgy. The data confirmed our previous suggestion that testicular tissue plays an important role in secondary sex determination in protandrous black porgy.<\/jats:p>","DOI":"10.3389\/fgene.2022.816955","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T23:59:19Z","timestamp":1648511959000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["The Ovarian Transcriptome at the Early Stage of Testis Removal-Induced Male-To-Female Sex Change in the Protandrous Black Porgy Acanthopagrus schlegelii"],"prefix":"10.3389","volume":"13","author":[{"given":"Peng-Wei","family":"Tseng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guan-Chung","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Lun","family":"Kuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung-Che","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching-Fong","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1002\/jez.1047","article-title":"Sex Reversal in Nile tilapia (Oreochromis niloticus) Using a Nonsteroidal Aromatase Inhibitor","volume":"290","author":"Afonso","year":"2001","journal-title":"J. Exp. Zool."},{"key":"B2","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.cbpa.2010.03.015","article-title":"Expression of Three Gonadotropin Subunits and Gonadotropin Receptor mRNA during Male-To-Female Sex Change in the Cinnamon Clownfish, Amphiprion melanopus","volume":"156","author":"An","year":"2010","journal-title":"Comp. Biochem. Physiol. A: Mol. Integr. Physiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"e1001899","DOI":"10.1371\/journal.pbio.1001899","article-title":"Sex Determination: Why So many Ways of Doing it?","volume":"12","author":"Bachtrog","year":"2014","journal-title":"Plos. Biol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12864-017-3953-6","article-title":"Transcriptomic Analysis of the Differentiating Ovary of the Protogynous Ricefield Eel Monopterus albus","volume":"18","author":"Cai","year":"2017","journal-title":"BMC Genomics"},{"key":"B5","doi-asserted-by":"publisher","first-page":"5825","DOI":"10.1093\/molbev\/msab293","article-title":"eggNOG-Mapper V2: Functional Annotation, Orthology Assignments, and Domain Prediction at the Metagenomic Scale","volume":"38","author":"Cantalapiedra","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep35461","article-title":"Sex Change in Clownfish: Molecular Insights from Transcriptome Analysis","volume":"6","author":"Casas","year":"2016","journal-title":"Sci. Rep."},{"key":"B7","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1002\/(sici)1097-010x(19980201)280:2<165:aid-jez7>3.0.co;2-m","article-title":"Estradiol-17\u03b2 Stimulates Aromatase Activity and Reversible Sex Change in Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"280","author":"Chang","year":"1998","journal-title":"J. Exp. Zool."},{"key":"B8","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/0044-8486(90)90187-R","article-title":"Annual Cycle of Gonadal Histology and Steroid Profiles in the Juvenile Males and Adult Females of the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"91","author":"Chang","year":"1990","journal-title":"Aquaculture"},{"key":"B9","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1006\/gcen.1995.1166","article-title":"Stimulation of Spermatogenesis or of Sex Reversal According to the Dose of Exogenous Estradiol-17\u03b2 in Juvenile Males of Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"100","author":"Chang","year":"","journal-title":"Gen. Comp. Endocrinol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1051\/rnd:19950310","article-title":"A Microencapsulated Analog of LH-RH Accelerates Maturation but without Stimulating Sex Reversal in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"35","author":"Chang","year":"","journal-title":"Reprod. Nutr. Dev."},{"key":"B11","first-page":"197","article-title":"The Endocrine Mechanism of Sex Reversal in the Protandrous Black Porgy, Acanthopagrus schlegeli: a Review","volume":"40","author":"Chang","year":"1997","journal-title":"Chin. J. Physiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"1785","DOI":"10.1210\/me.2014-1061","article-title":"Targeted Gene Disruption in Zebrafish Reveals Noncanonical Functions of LH Signaling in Reproduction","volume":"28","author":"Chu","year":"2014","journal-title":"Mol. Endocrinol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1210\/me.2015-1126","article-title":"Gonadotropin Signaling in Zebrafish Ovary and Testis Development: Insights from Gene Knockout Study","volume":"29","author":"Chu","year":"2015","journal-title":"Mol. Endocrinol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"dev172734","DOI":"10.1242\/dev.172734","article-title":"Cyclin B2 Is Required for Progression through Meiosis in Mouse Oocytes","volume":"146","author":"Daldello","year":"2019","journal-title":"Development"},{"key":"B15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1467-2979.2007.00266.x","article-title":"Functional Hermaphroditism in Teleosts","volume":"9","author":"De Mitcheson","year":"2008","journal-title":"Fish Fish."},{"key":"B16","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1023\/B:FISH.0000030499.77509.23","article-title":"Estradiol, Aromatase and Steroid Receptors Involved in the Sex Change of Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"28","author":"Du","year":"2003","journal-title":"Fish Physiol. Biochem."},{"key":"B17","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1095\/biolreprod.104.033159","article-title":"Seasonal Profiles of Brain and Pituitary Gonadotropin-Releasing Hormone and Plasma Luteinizing Hormone in Relation to Sex Change of Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"72","author":"Du","year":"2005","journal-title":"Biol. Reprod."},{"key":"B18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2014\/291067","article-title":"Gonadal Transcriptome Analysis of Male and Female Olive Flounder (Paralichthys olivaceus)","volume":"2014","author":"Fan","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"B19","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.ygcen.2013.05.024","article-title":"Environmental Stress-Induced Testis Differentiation: Androgen as a By-Product of Cortisol Inactivation","volume":"192","author":"Fernandino","year":"2013","journal-title":"Gen. Comp. Endocrinol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"830","DOI":"10.1038\/266830a0","article-title":"Monogamy and Sex Change by Aggressive Dominance in Coral Reef Fish","volume":"266","author":"Fricke","year":"1977","journal-title":"Nature"},{"key":"B21","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1111\/j.1469-7998.1994.tb01569.x","article-title":"Historical Aspects of Protandrous Sex Change in the Anemonefish Amphiprion melanopus (Pomacentridae, Teleostei)","volume":"232","author":"Godwin","year":"1994","journal-title":"J. Zoolog."},{"key":"B22","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length Transcriptome Assembly from RNA-Seq Data without a Reference Genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1002\/(sici)1098-2795(199910)54:2<154:aid-mrd7>3.0.co;2-5","article-title":"Involvement of Estrogens in the Process of Sex Differentiation in Two Fish Species: the Rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus)","volume":"54","author":"Guiguen","year":"1999","journal-title":"Mol. Reprod. Dev."},{"key":"B24","doi-asserted-by":"publisher","first-page":"113840","DOI":"10.1016\/j.ygcen.2021.113840","article-title":"Potential Role of DNA Methylation of cyp19a1a Promoter during Sex Change in Protogynous orange-spotted Grouper, Epinephelus coioides","volume":"311","author":"Guo","year":"2021","journal-title":"Gen. Comp. Endocrinol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-017-13688-3","article-title":"The Translation of Cyclin B1 and B2 Is Differentially Regulated during Mouse Oocyte Reentry into the Meiotic Cell Cycle","volume":"7","author":"Han","year":"2017","journal-title":"Sci. Rep."},{"key":"B26","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1095\/biolreprod.102.015040","article-title":"Differential Messenger RNA Transcription of Androgen Receptor and Estrogen Receptor in Gonad in Relation to the Sex Change in Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"69","author":"He","year":"","journal-title":"Biol. Reprod."},{"key":"B27","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1159\/000074354","article-title":"Differential Dmrt1 Transcripts in Gonads of the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"101","author":"He","year":"","journal-title":"Cytogenet. Genome Res."},{"key":"B28","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.aaf.2020.09.007","article-title":"Gonadal Transcriptomic Analysis and Differentially Expressed Genes between the Testes and Ovaries in Trachinotus ovatus","volume":"7","author":"He","year":"2020","journal-title":"Aquacult. Fish"},{"key":"B29","doi-asserted-by":"publisher","first-page":"304","DOI":"10.3389\/fendo.2019.00304","article-title":"New Insights into the Role of Follicle-Stimulating Hormone in Sex Differentiation of the Protogynous orange-spotted Grouper, Epinephelus coioides","volume":"10","author":"Huang","year":"2019","journal-title":"Front. Endocrinol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"D309","DOI":"10.1093\/nar\/gky1085","article-title":"eggNOG 5.0: a Hierarchical, Functionally and Phylogenetically Annotated Orthology Resource Based on 5090 Organisms and 2502 Viruses","volume":"47","author":"Huerta-Cepas","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"B31","doi-asserted-by":"publisher","first-page":"W395","DOI":"10.1093\/nar\/gkaa434","article-title":"The Galaxy Platform for Accessible, Reproducible and Collaborative Biomedical Analyses: 2020 Update","volume":"48","author":"Jalili","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/(sici)1098-2795(200005)56:1<1:aid-mrd1>3.0.co;2-3","article-title":"Aromatase Inhibitor and 17\u03b1-methyltestosterone Cause Sex-Reversal from Genetical Females to Phenotypic Males and Suppression of P450 Aromatase Gene Expression in Japanese Flounder (Paralichthys olivaceus)","volume":"56","author":"Kitano","year":"2000","journal-title":"Mol. Reprod. Dev."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1503","DOI":"10.1210\/en.2008-0569","article-title":"Sex Change in the Gobiid Fish Is Mediated through Rapid Switching of Gonadotropin Receptors from Ovarian to Testicular Portion or Vice Versa","volume":"150","author":"Kobayashi","year":"2009","journal-title":"Endocrinology"},{"key":"B34","doi-asserted-by":"publisher","first-page":"1030","DOI":"10.1095\/biolreprod.109.080986","article-title":"Sexually Dimorphic Expression of Gonadotropin Subunits in the Pituitary of Protogynous Honeycomb Grouper (Epinephelus merra): Evidence that Follicle-Stimulating Hormone (FSH) Induces Gonadal Sex Change","volume":"82","author":"Kobayashi","year":"2010","journal-title":"Biol. Reprod."},{"key":"B35","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1002\/jez.1402520207","article-title":"Human Chorionic Gonadotropin (hCG) Induces Gonad Reversal in a Protogynous Fish, the Bluehead Wrasse, Thalassoma bifasciatum (Teleostei, Labridae)","volume":"252","author":"Koulish","year":"1989","journal-title":"J. Exp. Zool."},{"key":"B36","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1111\/j.1095-8649.1997.tb01669.x","article-title":"Stimulation of Testicular Function by Exogenous Testosterone in Male Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"51","author":"Lau","year":"1997","journal-title":"J. Fish Biol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1006\/gcen.2000.7499","article-title":"Profiles of Gonadal Development, Sex Steroids, Aromatase Activity, and Gonadotropin II in the Controlled Sex Change of Protandrous Black Porgy, Acanthopagrus schlegeli Bleeker","volume":"119","author":"Lee","year":"2000","journal-title":"Gen. Comp. Endocrinol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1002\/jez.1122","article-title":"Sex Change in the Protandrous Black Porgy, Acanthopagrus schlegeli: A Review in Gonadal Development, Estradiol, Estrogen Receptor, Aromatase Activity and Gonadotropin","volume":"290","author":"Lee","year":"2001","journal-title":"J. Exp. Zool."},{"key":"B39","doi-asserted-by":"publisher","first-page":"1749","DOI":"10.1095\/biolreprod66.6.1749","article-title":"Aromatase Inhibitors Block Natural Sex Change and Induce Male Function in the Protandrous Black Porgy, Acanthopagrus schlegeli Bleeker: Possible Mechanism of Natural Sex Change","volume":"66","author":"Lee","year":"2002","journal-title":"Biol. Reprod."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1095\/biolreprod.104.030221","article-title":"Estradiol-17\u03b2 Induced a Reversible Sex Change in the Fingerlings of Protandrous Black Porgy, Acanthopagrus schlegeli Bleeker: The Possible Roles of Luteinizing Hormone in Sex Change","volume":"71","author":"Lee","year":"2004","journal-title":"Biol. Reprod."},{"key":"B41","first-page":"302","article-title":"Development of Ovarian Tissue and Female Germ Cells in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"47","author":"Lee","year":"2008","journal-title":"Zool. Stud."},{"key":"B42","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1002\/ar.21339","article-title":"Development of the Genital Duct System in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"294","author":"Lee","year":"2011","journal-title":"Anat. Rec."},{"key":"B43","doi-asserted-by":"publisher","first-page":"709","DOI":"10.3389\/fgene.2020.00709","article-title":"Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream (Acanthopagrus latus)","volume":"11","author":"Li","year":"2020","journal-title":"Front. Genet."},{"key":"B44","doi-asserted-by":"publisher","first-page":"3007","DOI":"10.3390\/cells10113007","article-title":"Involvement of Transforming Growth Factor Beta Family Genes in Gonadal Differentiation in Japanese Eel, Anguilla japonica, According to Sex-Related Gene Expressions","volume":"10","author":"Lin","year":"2021","journal-title":"Cells"},{"key":"B45","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13293-015-0044-8","article-title":"Large-scale Transcriptome Sequencing Reveals Novel Expression Patterns for Key Sex-Related Genes in a Sex-Changing Fish","volume":"6","author":"Liu","year":"2015","journal-title":"Biol. Sex. Differ."},{"key":"B46","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation of Fold Change and Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1152\/physiolgenomics.00088.2014","article-title":"Gonadal Transcriptomic Analysis of Yellow Catfish (Pelteobagrus fulvidraco): Identification of Sex-Related Genes and Genetic Markers","volume":"46","author":"Lu","year":"2014","journal-title":"Physiol. Genomics"},{"key":"B48","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1095\/biolreprod.113.110569","article-title":"Administration of Follicle-Stimulating Hormone In Vivo Triggers Testicular Recrudescence of Juvenile European Sea Bass (Dicentrarchus labrax)1","volume":"90","author":"Maz\u00f3n","year":"2014","journal-title":"Biol. Reprod."},{"key":"B49","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1007\/s002650050504","article-title":"Bi-directional Sex Change in a Coral-Dwelling Goby","volume":"43","author":"Munday","year":"1998","journal-title":"Behav. Ecol. Sociobiol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"3136","DOI":"10.1210\/en.2013-2060","article-title":"Loss of Follicle-Stimulating Hormone Receptor Function Causes Masculinization and Suppression of Ovarian Development in Genetically Female Medaka","volume":"155","author":"Murozumi","year":"2014","journal-title":"Endocrinology"},{"key":"B51","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1126\/science.1185473","article-title":"Identification of Germline Stem Cells in the Ovary of the Teleost Medaka","volume":"328","author":"Nakamura","year":"2010","journal-title":"Science"},{"key":"B52","doi-asserted-by":"publisher","first-page":"970","DOI":"10.1095\/biolreprod.107.062299","article-title":"Follicle-Stimulating Hormone Induces Spermatogenesis Mediated by Androgen Production in Japanese Eel, Anguilla japonica","volume":"77","author":"Ohta","year":"2007","journal-title":"Biol. Reprod."},{"key":"B53","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1093\/biolre\/ioy228","article-title":"Gonadal Response of Juvenile Protogynous Grouper (Epinephelus fuscoguttatus) to Long-Term Recombinant Follicle-Stimulating Hormone Administration","volume":"100","author":"Palma","year":"2019","journal-title":"Biol. Reprod."},{"key":"B54","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon Provides Fast and Bias-Aware Quantification of Transcript Expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat. Methods"},{"key":"B55","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1002\/jez.1402700304","article-title":"Brief Treatment with an Aromatase Inhibitor during Sex Differentiation Causes Chromosomally Female salmon to Develop as normal, Functional Males","volume":"270","author":"Piferrer","year":"1994","journal-title":"J. Exp. Zool."},{"key":"B56","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1038\/35087082","article-title":"Mammalian STAG3 Is a Cohesin Specific to Sister Chromatid Arms in Meiosis I","volume":"3","author":"Prieto","year":"2001","journal-title":"Nat. Cel Biol."},{"key":"B57","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1093\/bioinformatics\/btv351","article-title":"BUSCO: Assessing Genome Assembly and Annotation Completeness with Single-Copy Orthologs","volume":"31","author":"Sim\u00e3o","year":"2015","journal-title":"Bioinformatics"},{"key":"B58","doi-asserted-by":"publisher","first-page":"3994","DOI":"10.1210\/en.2016-1356","article-title":"Evolution of the Hypothalamic-Pituitary-Gonadal axis Regulation in Vertebrates Revealed by Knockout Medaka","volume":"157","author":"Takahashi","year":"2016","journal-title":"Endocrinology"},{"key":"B59","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/0016-6480(74)90178-6","article-title":"Effect of Mammalian Luteinizing Hormone on the Natural Sex Reversal of the rice-field Eel, Monopterus albus (Zuiew)","volume":"24","author":"Tang","year":"1974","journal-title":"Gen. Comp. Endocrinol."},{"key":"B60","doi-asserted-by":"publisher","first-page":"e63604","DOI":"10.1371\/journal.pone.0063604","article-title":"Characterization of Gonadal Transcriptomes from Nile tilapia (Oreochromis niloticus) Reveals Differentially Expressed Genes","volume":"8","author":"Tao","year":"2013","journal-title":"PloS one"},{"key":"B61","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.aaf.2020.09.004","article-title":"Development and Organisation of Gonadal Steroidogenesis in Bony Fishes - A Review","volume":"6","author":"Tenugu","year":"2021","journal-title":"Aquacult. Fish."},{"key":"B62","doi-asserted-by":"publisher","first-page":"1797","DOI":"10.1016\/j.cub.2003.09.032","article-title":"The Meiosis I-To-Meiosis II Transition in Mouse Oocytes Requires Separase Activity","volume":"13","author":"Terret","year":"2003","journal-title":"Curr. Biol."},{"key":"B63","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1159\/000449297","article-title":"Bending Genders: the Biology of Natural Sex Change in Fish","volume":"10","author":"Todd","year":"2016","journal-title":"Sex. Dev."},{"key":"B64","doi-asserted-by":"publisher","first-page":"eaaw7006","DOI":"10.1126\/sciadv.aaw7006","article-title":"Stress, Novel Sex Genes, and Epigenetic Reprogramming Orchestrate Socially Controlled Sex Change","volume":"5","author":"Todd","year":"2019","journal-title":"Sci. Adv."},{"key":"B65","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-018-21992-9","article-title":"The Transcriptomic Signature of Different Sexes in Two Protogynous Hermaphrodites: Insights into the Molecular Network Underlying Sex Phenotype in Fish","volume":"8","author":"Tsakogiannis","year":"2018","journal-title":"Sci. Rep."},{"key":"B66","doi-asserted-by":"publisher","first-page":"199","DOI":"10.2307\/1542090","article-title":"Social Control of Sex Change in the Bluehead Wrasse, Thalassoma bifasciatum (Pisces: Labridae)","volume":"181","author":"Warner","year":"1991","journal-title":"Biol. Bull."},{"key":"B67","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1023\/B:FISH.0000030520.60240.cd","article-title":"The Involvement of Gonadotropin Receptors in Sex Reversal: Expression, Distribution and Regulation of Gonadal FSH and LH Receptors in the Gilthead Seabream","volume":"28","author":"Wong","year":"2003","journal-title":"Fish Physiol. Biochem."},{"key":"B68","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1095\/biolreprod.109.077362","article-title":"wnt4a Is Associated with the Development of Ovarian Tissue in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"81","author":"Wu","year":"2009","journal-title":"Biol. Reprod."},{"key":"B69","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.ygcen.2017.01.033","article-title":"Primary Males Guide the Femaleness through the Regulation of Testicular Dmrt1 and Ovarian cyp19a1a in Protandrous Black Porgy","volume":"261","author":"Wu","year":"2018","journal-title":"Gen. Comp. Endocrinol."},{"key":"B70","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1196\/annals.1327.026","article-title":"Current Status of Genetic and Endocrine Factors in the Sex Change of Protandrous Black Porgy, Acanthopagrus schlegeli (Teleostean)","volume":"1040","author":"Wu","year":"2005","journal-title":"Ann. N.Y Acad. Sci"},{"key":"B71","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1095\/biolreprod.107.062612","article-title":"The Expression of nr0b1 and nr5a4 during Gonad Development and Sex Change in Protandrous Black Porgy Fish, Acanthopagrus schlegeli","volume":"78","author":"Wu","year":"","journal-title":"Biol. Reprod."},{"key":"B72","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1095\/biolreprod.108.069146","article-title":"Dual Roles of cyp19a1a in Gonadal Sex Differentiation and Development in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"79","author":"Wu","year":"","journal-title":"Biol. Reprod."},{"key":"B73","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1095\/biolreprod.110.084681","article-title":"The Expression of amh and amhr2 Is Associated with the Development of Gonadal Tissue and Sex Change in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"83","author":"Wu","year":"","journal-title":"Biol. Reprod."},{"key":"B74","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.ygcen.2009.11.003","article-title":"Sex Differentiation and Sex Change in the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"167","author":"Wu","year":"","journal-title":"Gen. Comp. Endocrinol."},{"key":"B75","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1095\/biolreprod.111.095695","article-title":"Testicular dmrt1 Is Involved in the Sexual Fate of the Ovotestis in the Protandrous Black Porgy","volume":"86","author":"Wu","year":"2012","journal-title":"Biol. Reprod."},{"key":"B76","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1095\/biolreprod.114.126953","article-title":"The Potential Role of Amh to Prevent Ectopic Female Development in Testicular Tissue of the Protandrous Black Porgy, Acanthopagrus schlegelii","volume":"92","author":"Wu","year":"2015","journal-title":"Biol. Reprod."},{"key":"B77","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1095\/biolreprod.115.137463","article-title":"The Testis Is a Primary Factor that Contributes to Epigenetic Modifications in the Ovaries of the Protandrous Black Porgy, Acanthopagrus schlegeli","volume":"94","author":"Wu","year":"2016","journal-title":"Biol. Reprod."},{"key":"B78","doi-asserted-by":"publisher","first-page":"e0186991","DOI":"10.1371\/journal.pone.0186991","article-title":"Robust gdf9 and bmp15 Expression in the Oocytes of Ovotestes through the Figla-independent Pathway in the Hermaphroditic Black Porgy, Acanthopagrus schlegelii","volume":"12","author":"Wu","year":"2017","journal-title":"PloS one"},{"key":"B79","doi-asserted-by":"publisher","first-page":"111069","DOI":"10.1016\/j.mce.2020.111069","article-title":"Molecular and Cellular Regulation on Sex Change in Hermaphroditic Fish, with a Special Focus on Protandrous Black Porgy, Acanthopagrus schlegelii","volume":"520","author":"Wu","year":"2021","journal-title":"Mol. Cell Endocrinol."},{"key":"B81","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.ygeno.2018.11.006","article-title":"A Comparative Transcriptomic Study on Developmental Gonads Provides Novel Insights into Sex Change in the Protandrous Black Porgy (Acanthopagrus schlegelii)","volume":"111","author":"Zhang","year":"2019","journal-title":"Genomics"},{"key":"B82","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/s10695-008-9219-0","article-title":"Molecular Mechanisms Underlying Sex Change in Hermaphroditic Groupers","volume":"36","author":"Zhou","year":"2010","journal-title":"Fish. Physiol. Biochem."},{"key":"B83","doi-asserted-by":"publisher","first-page":"113544","DOI":"10.1016\/j.ygcen.2020.113544","article-title":"Fish Reproductive Biology - Reflecting on Five Decades of Fundamental and Translational Research","volume":"300","author":"Zohar","year":"2021","journal-title":"Gen. Comp. Endocrinol."}],"container-title":["Frontiers in Genetics"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fgene.2022.816955\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T23:59:25Z","timestamp":1648511965000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fgene.2022.816955\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,23]]},"references-count":82,"alternative-id":["10.3389\/fgene.2022.816955"],"URL":"https:\/\/doi.org\/10.3389\/fgene.2022.816955","relation":{},"ISSN":["1664-8021"],"issn-type":[{"value":"1664-8021","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,23]]},"article-number":"816955"}}