{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T09:15:58Z","timestamp":1769246158893,"version":"3.49.0"},"reference-count":57,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007162","name":"Guangdong Science and Technology Department","doi-asserted-by":"publisher","award":["2018B020203001 2020B020222002 2020A1515011277"],"award-info":[{"award-number":["2018B020203001 2020B020222002 2020A1515011277"]}],"id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>Hyperpigmentation of the visceral peritoneum (HVP) has been becoming one of the most challenging problems in yellow-feathered chicken production, which seriously affected chicken carcass quality traits. Detecting which genes dominantly impact pigmentation in the peritoneum tissues is of great benefit to the genetic improvement of HVP. To investigate the genetic mechanism of HVP in yellow-feathered broilers, genome-wide association studies (GWASs) were conducted in the F<jats:sub>2<\/jats:sub> generation of a cross broiler population with 395 birds. A total of 115,706 single-nucleotide polymorphisms (SNPs) of 122,415 were retained to identify quantitative trait loci (QTL) associated to HVP in chicken. The GWAS results based on the logistic mixed model (LMM) revealed that a narrow genomic location on chromosomes 1 (49.2\u201351.3\u00a0Mb) was significantly associated (<jats:italic>p<\/jats:italic> \u2264 4.32 \u00d7 10<jats:sup>\u22127<\/jats:sup>) with HVP, which contained 23 SNP makers related to 14 functional genes (<jats:italic>MFNG<\/jats:italic>, <jats:italic>POLDIP3<\/jats:italic>, <jats:italic>POLR2F<\/jats:italic>, <jats:italic>PICK1<\/jats:italic>, <jats:italic>PDXP<\/jats:italic>, <jats:italic>SGSM3<\/jats:italic>, <jats:italic>RANGAP1<\/jats:italic>, <jats:italic>MYH9<\/jats:italic>, <jats:italic>RPL3<\/jats:italic>, <jats:italic>GALP3<\/jats:italic>, <jats:italic>LGALS1<\/jats:italic>, <jats:italic>MICALL1<\/jats:italic>, <jats:italic>ATF4<\/jats:italic>, and <jats:italic>CYP2D6<\/jats:italic>). Four highly associated (<jats:italic>p<\/jats:italic> &amp;lt; 10<jats:sup>\u22125<\/jats:sup>) haplotype blocks of 0.80\u00a0kb (two SNPs), 0.06\u00a0kb (two SNPs), 0.95\u00a0kb (two SNPs), and 0.03\u00a0kb (two SNPs) were identified with two, two, four, and four distinct haplotypes, respectively. As a melanoma-associated gene, <jats:italic>CYP2D6<\/jats:italic> were also possibly involved in the development of HVP occurring in chicken with two significant variations (rs314284996 and rs317955795) in the promoter regions. Further tests revealed that the expression of <jats:italic>CYP2D6<\/jats:italic> was obviously higher in the visceral peritoneum tissue of chicken with HVP than that in the normal group (<jats:italic>p<\/jats:italic> &amp;lt; 0.05). Our results provide a novel clue to understand the genetic mechanism of HVP generation in chicken, and the mapped QTL or candidate genes might serve for genomic selection to improve carcass quality in the yellow-feathered chicken industry.<\/jats:p>","DOI":"10.3389\/fgene.2022.820297","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T06:19:36Z","timestamp":1646115576000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["Genome-Wide Association Studies Provide Insight Into the Genetic Determination for Hyperpigmentation of the Visceral Peritoneum in Broilers"],"prefix":"10.3389","volume":"13","author":[{"given":"Guangyuan","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianfei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Qu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dingming","family":"Shu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinzheng","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenglong","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3835\/plantgenome2019.05.0036","article-title":"Comparing Single-SNP, Multi-SNP, and Haplotype-Based Approaches in Association Studies for Major Traits in Barley","volume":"12","author":"Abed","year":"2019","journal-title":"Plant Genome"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1101\/gr.094052.109","article-title":"Fast Model-Based Estimation of Ancestry in Unrelated Individuals","volume":"19","author":"Alexander","year":"2009","journal-title":"Genome Res."},{"key":"B3","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1080\/00071668.2011.635637","article-title":"Genetics of Hyperpigmentation Associated with the Fibromelanosis Gene (Fm) and Analysis of Growth and Meat Quality Traits in Crosses of Native Indian Kadaknath Chickens and Non-indigenous Breeds","volume":"52","author":"Arora","year":"2011","journal-title":"Br. 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