{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:28:50Z","timestamp":1769560130771,"version":"3.49.0"},"reference-count":31,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>Many economic crustacean species have sex dimorphisms during their growth. Exploring the sex determination system and developing sex-specific molecular marker(s) are very helpful for carrying out sex control breeding, and next-generation sequencing has been used as an efficient way to explore them in recent years. In this study, first, the genetic sex determination system of <jats:italic>P. clarkii<\/jats:italic> was explored as an XX\/XY system by analyzing the 2b-RAD sequencing data. Furthermore, DNA samples of male and female individuals from a <jats:italic>P. clarkii<\/jats:italic> family were pooled separately for whole-genome resequencing. Based on the data of whole-genome resequencing, the 9,163 male- and female-specific bias sites with higher feasibility were obtained based on the assumption of the XX\/XY sex determination system, and four sites were selected to design the sex-specific marker primers. One efficient sex-specific marker was identified with a sex discrimination rate of 99.49% (195\/196) when applied to five different geographical groups with 196 individuals. The results of this study would provide a foundation for the realization of <jats:italic>P. clarkii<\/jats:italic> sex control and could provide some reference for investigating the sex determination system and sex molecular marker(s) of other crustacean species based on next-generation sequencing data.<\/jats:p>","DOI":"10.3389\/fgene.2022.850983","type":"journal-article","created":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T21:57:03Z","timestamp":1646171823000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Exploration of an XX\/XY Sex Determination System and Development of PCR-Based Sex-specific Markers in Procambarus clarkii Based on Next-Generation Sequencing Data"],"prefix":"10.3389","volume":"13","author":[{"given":"Yudong","family":"Shen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qishuai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weimin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhe","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,3,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/gigascience\/gix120","article-title":"SOAPnuke: a MapReduce Acceleration-Supported Software for Integrated Quality Control and Preprocessing of High-Throughput Sequencing Data","volume":"7","author":"Chen","year":"2018","journal-title":"GigaScience"},{"key":"B2","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/S0044-8486(02)00057-1","article-title":"Sex Determination and Sex Differentiation in Fish: an Overview of Genetic, Physiological, and Environmental Influences","volume":"208","author":"Devlin","year":"2002","journal-title":"Aquaculture"},{"key":"B3","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.ygeno.2019.03.003","article-title":"Identification of Male-specific SNP Markers and Development of PCR-Based Genetic Sex Identification Technique in Crucifix Crab (Charybdis Feriatus) with Implication of an XX\/XY Sex Determination System","volume":"112","author":"Fang","year":"2020","journal-title":"Genomics"},{"key":"B4","doi-asserted-by":"publisher","first-page":"734749","DOI":"10.1016\/j.aquaculture.2019.734749","article-title":"A Rapid and Reliable Method for Identifying Genetic Sex in Obscure Pufferfish (Takifugu Obscurus)","volume":"519","author":"Gao","year":"2020","journal-title":"Aquaculture"},{"key":"B5","first-page":"239","article-title":"Procambarus in North America and Elsewhere","volume-title":"Freshwater Crayfish: Biology, Management and Exploitation","author":"Huner","year":"1988"},{"key":"B6","doi-asserted-by":"publisher","first-page":"e6214","DOI":"10.7717\/peerj.6214","article-title":"Reproductive Pattern and Population Dynamics of Commercial Red Swamp Crayfish (Procambarus clarkii) from China: Implications for Sustainable Aquaculture Management","volume":"7","author":"Jin","year":"2019","journal-title":"Peer J."},{"key":"B7","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.gene.2014.04.034","article-title":"Identification of Sex-Linked DNA Markers by AFLP in Portunus Trituberculatus","volume":"544","author":"Lai","year":"2014","journal-title":"Gene"},{"key":"B8","doi-asserted-by":"publisher","first-page":"917","DOI":"10.4194\/1303-2712-v12_4_19","article-title":"Length weight relationship and condition factor of giant freshwater prawn Macrobrachium rosenbergii (de man, 1879) based on developmental stages, culture stages and sex","volume":"12","author":"Lalrinsanga","year":"2012","journal-title":"Turk. 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