{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:15:15Z","timestamp":1787026515193,"version":"build-2736575974"},"reference-count":101,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Cobia (\n                    <jats:italic>Rachycentron canadum<\/jats:italic>\n                    ) is a species of fish with high commercial potential particularly due to fast growth rates. The evidence of sexual size dimorphism favoring females indicate potential benefits in having a monosex culture. However, the involvement of genetic factors responsible for sexual development and gonadal maintenance that produces phenotypic sex in cobia is largely unknown.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    In the present study, we performed transcriptome sequencing of cobia to identify sex-biased significantly differentially expressed genes (DEGs) in testes and ovaries. The reliability of the gonad transcriptome data was validated by qPCR analysis of eight selected significantly differential expressed sex-related candidate genes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    This comparative gonad transcriptomic analysis revealed that 7,120 and 4,628 DEGs are up-regulated in testes or ovaries, respectively. Further functional annotation analyses identified 76 important candidate genes involved in sex determination cascades or sex differentiation, including 42 known testis-biased DEGs (\n                    <jats:italic>dmrt1<\/jats:italic>\n                    ,\n                    <jats:italic>amh<\/jats:italic>\n                    and\n                    <jats:italic>sox9 etc.<\/jats:italic>\n                    ), and 34 known ovary-biased DEGs (\n                    <jats:italic>foxl2<\/jats:italic>\n                    ,\n                    <jats:italic>sox3<\/jats:italic>\n                    and\n                    <jats:italic>cyp19a etc.<\/jats:italic>\n                    ). Moreover, eleven significantly enriched pathways functionally related to sex determination and sex differentiation were identified, including Wnt signaling pathway, oocyte meiosis, the TGF-beta signaling pathway and MAPK signaling pathway.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    This work represents the first comparative gonad transcriptome study in cobia. The putative sex-associated DEGs and pathways provide an important molecular basis for further investigation of cobia\u2019s sex determination, gonadal development as well as potential control breeding of monosex female populations for a possible aquaculture setting.\n                  <\/jats:p>","DOI":"10.3389\/fgene.2023.1128943","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T01:03:26Z","timestamp":1680743006000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":13,"title":["Comparative gonad transcriptome analysis in cobia (Rachycentron canadum)"],"prefix":"10.3389","volume":"14","author":[{"given":"Xueyan","family":"Shen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Y\u00e1\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giana","family":"Bastos Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi Weng Josiah","family":"Poon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derick","family":"Foster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge F.","family":"Alarcon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose A.","family":"Domingos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq\u2014A Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"bioinformatics"},{"key":"B2","volume-title":"De novo transcriptome assembly and functional annotation of cobia (","author":"Barbosa","year":"2020"},{"key":"B3","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1095\/biolreprod.105.041830","article-title":"Large-scale temporal gene expression profiling during gonadal differentiation and early gametogenesis in rainbow trout","volume":"73","author":"Baron","year":"2005","journal-title":"Biol. reproduction"},{"key":"B4","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1111\/jwas.12810","article-title":"A review on cobia, Rachycentron canadum, aquaculture","volume":"52","author":"Benetti","year":"2021","journal-title":"J. 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