{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T18:03:44Z","timestamp":1771524224826,"version":"3.50.1"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> Pediatric Crohn\u2019s disease is a chronic inflammatory condition that affects the digestive system in children and adolescents. It is characterized by symptoms such as abdominal pain, diarrhea, weight loss, and malnutrition, and can also cause complications like growth delays and delayed puberty. However, diagnosing pediatric Crohn\u2019s disease can be difficult, especially when it comes to non-invasive methods.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In this study, we developed a diagnostic model using RNA-seq to analyze gene expression in ileal biopsy samples from children with Crohn\u2019s disease and non-pediatric Crohn\u2019s controls.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Our results showed that pediatric Crohn\u2019s disease is associated with altered expression of genes involved in immune response, inflammation, and tissue repair. We validated our findings using two independent datasets from the Gene Expression Omnibus (GEO) database, as well as through one prospective independent dataset, and found that our model had a high accuracy rate.<\/jats:p><jats:p><jats:bold>Discussion:<\/jats:bold> These findings suggest the possibility of non-invasive diagnosis for pediatric Crohn\u2019s disease and may inform the development of targeted therapies for this condition.<\/jats:p>","DOI":"10.3389\/fgene.2023.1142326","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T06:35:39Z","timestamp":1677652539000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Developing a non-invasive diagnostic model for pediatric Crohn\u2019s disease using RNA-seq analysis"],"prefix":"10.3389","volume":"14","author":[{"given":"Bin","family":"He","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhua","family":"Shu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,3,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1097\/MCG.0b013e318247c32f","article-title":"Natural history of pediatric-onset inflammatory bowel disease: A systematic review","volume":"46","author":"Abraham","year":"2012","journal-title":"J. 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