{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:09:34Z","timestamp":1790863774486,"version":"4.1.0"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"\n Background<\/jats:title>\n \n Malaria in pregnancy is a major public health issue, particularly among vulnerable populations in malaria-endemic sub-Saharan African countries. To mitigate its risks, WHO recommends sulphadoxine-pyrimethamine (SP) for chemoprevention and artemisinin-based combination therapy (ACT) to treat uncomplicated\n Plasmodium falciparum<\/jats:italic>\n malaria. These interventions have helped to alleviate the risk associated with malaria in pregnancy; however, in the context of the emergence of SP- and ACT-resistant\n P. falciparum<\/jats:italic>\n , maintained efficacy is under threat. Molecular surveillance is a reliable tool to monitor the emergence of resistance where molecular markers are known. Thus, the objective of the study was to use a multiplexed amplicon Oxford Nanopore sequencing approach to assess the molecular markers for antimalarial resistance among pregnant women in Nigeria.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Dried blood spots (DBS) were collected from pregnant women who received IPTp-SP at the enrollment and follow-up visits.\n P. falciparum<\/jats:italic>\n genomic DNA was extracted by the Chelex\n \u00ae<\/jats:sup>\n method and\n Pf<\/jats:italic>\n 18S qPCR was used to detect parasite DNA in each sample. With nested PCR assays, fragments of\n Pfdhps<\/jats:italic>\n ,\n Pfdhfr<\/jats:italic>\n ,\n Pfmdr1<\/jats:italic>\n ,\n Pfcrt<\/jats:italic>\n ,\n Pfk13<\/jats:italic>\n and\n Pfama1<\/jats:italic>\n genes were amplified and multiplexed amplicon-based sequencing was conducted on the minION Oxford Nanopore Technology.\n <\/jats:p>\n <\/jats:sec>\n \n Result<\/jats:title>\n \n In total, 251 pregnant women were enrolled in the study and 457 DBS samples were collected.\n P. falciparum<\/jats:italic>\n genomic DNA was detected in 12% (56\/457) of the samples, 31 at baseline and the remaining during the follow-up visits.\n Pfama1<\/jats:italic>\n ,\n pfk13<\/jats:italic>\n ,\n Pfdhps<\/jats:italic>\n ,\n Pfdhfr<\/jats:italic>\n ,\n Pfmdr1 and Pfcrt<\/jats:italic>\n were successfully sequenced in a single run. Notably, k13 artemisinin resistance mutations were absent, the frequencies of\n Pfdhfr<\/jats:italic>\n and\n Pfdhps<\/jats:italic>\n SP resistance haplotypes,\n IRN<\/jats:bold>\n for pyrimethamine resistance and I\n SG<\/jats:bold>\n KA\/IA\n G<\/jats:bold>\n KA associated with sulphadoxine resistance were 82% (36\/44) and 64% (27\/42), respectively, and the\n Pfcrt<\/jats:italic>\n CV\n IET<\/jats:bold>\n resistant haplotype was at approximately 22% (7\/32).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion and recommendations<\/jats:title>\n \n Here a multiplexed amplicon-based ONT assay established that triple mutant\n Pfdfhr<\/jats:italic>\n -IRN, double mutant\n Pfdhps<\/jats:italic>\n -SG haplotypes and the chloroquine sensitive strain were prevalent among pregnant women in Nigeria.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fgene.2024.1470156","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T10:51:49Z","timestamp":1728989509000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["ONT sequencing identifies a high prevalence of crt sensitive, triple mutant dhfr and single mutant dhps parasites within an ANC population in Nigeria"],"prefix":"10.3389","volume":"15","author":[{"given":"Adebanjo Jonathan","family":"Adegbola","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard","family":"Ndwiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Wamae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Osoti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluseye Oladotun","family":"Bolaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Bejon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynette Isabella","family":"Ochola-Oyier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"6","DOI":"10.3390\/diseases9010006","article-title":"Polymorphism analysis of pfmdr1 and pfcrt from Plasmodium falciparum isolates in northwestern Nigeria revealed the major markers associated with antimalarial resistance","volume":"9","author":"Adam","year":"2021","journal-title":"Diseases"},{"key":"B2","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1186\/s12936-023-04487-5","article-title":"A snapshot of the prevalence of dihydropteroate synthase-431V mutation and other sulfadoxine-pyrimethamine resistance markers in Plasmodium falciparum isolates in Nigeria","volume":"22","author":"Adegbola","year":"2023","journal-title":"Malar. 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