{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T11:12:35Z","timestamp":1771931555803,"version":"3.50.1"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The circRNA-miRNA axis is critically implicated in the pathogenesis of recurrent spontaneous abortion (RSA) by modulating trophoblast and decidual stromal cell functions. This study investigates the role of the miR-133a\/circ-104792 network in RSA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using qRT-PCR, we measured miR-133a and circ-104792 expression in chorion and decidua from 10 RSA patients and 10 controls. The functional effects on proliferation (CCK-8, EdU) and apoptosis (flow cytometry, TUNEL) were assessed in HTR-8\/SVneo and HESC cells following transfection with miR-133a mimics\/inhibitor or circ-104792. Direct targeting of Bcl-xL by miR-133a and its interaction with circ-104792 were validated via dual-luciferase reporter and RNA pull-down assays. Bcl-xL expression was evaluated by qRT-PCR and western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      miR-133a expression was (in chorion: p = 0.0008, 95% CI [2.85, 6.41]; in decidua: p = 0.0009, 95% CI [2.67, 6.03]) increased, while circ-104792 was significantly downregulated (in chorion: p = 0.0008, 95% CI [0.15, 0.34]; in decidua: p = 0.0007, 95% CI [0.13, 0.31]) in RSA tissues.\n                      <jats:italic>In vitro<\/jats:italic>\n                      , miR-133a overexpression inhibited cell proliferation, promoted apoptosis, and reduced Bcl-xL levels (in HTR-8\/SVneo: 0.46-fold vs. 1.00 in NC group, p = 0.0045; in HESCs: 0.49-fold vs. 1.00 in NC group, p = 0.0003). Conversely, circ-104792 overexpression enhanced proliferation, suppressed apoptosis, and increased Bcl-xL expression (in HTR-8\/SVneo: 2.17-fold vs. 1.00 in vector group, p = 0.0018; in HESCs: 1.94-fold vs. 1.00 in vector group, p = 0.0015). The dual-luciferase assay confirmed Bcl-xL as a direct target of miR-133a, and the RNA pull-down confirmed the miR-133a\/circ-104792 interaction. Critically, circ-104792 overexpression rescued the suppressive effects of miR-133a on proliferation and Bcl-xL expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings demonstrate that miR-133a promotes RSA-associated cellular dysfunction by targeting Bcl-xL, while circ-104792 acts as a ceRNA to sponge miR-133a, thereby antagonizing its effects. The miR-133a\/circ-104792\/Bcl-xL axis represents a potential key regulatory network in RSA, presenting potential novel targets for diagnosis and therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fgene.2026.1707900","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T22:10:23Z","timestamp":1770847823000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Circ-104792\/miR-133a\/Bcl-xL influences the proliferation and function of human trophoblastic and decidual stromal cells involved in recurrent abortion disease"],"prefix":"10.3389","volume":"17","author":[{"given":"Wenjuan","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xi\u2019an People\u2019s Hospital (Xi\u2019an Fourth Hospital)","place":["Xi\u2019an, Shaanxi, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Reproductive Medicine Center, Tangdu Hospital, Air Force Military Medical University","place":["Xi\u2019an, Shaanxi, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongli","family":"Zhu","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Xi\u2019an People\u2019s Hospital (Xi\u2019an Fourth Hospital)","place":["Xi\u2019an, Shaanxi, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panpan","family":"Shi","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Xi\u2019an People\u2019s Hospital (Xi\u2019an Fourth Hospital)","place":["Xi\u2019an, Shaanxi, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaochun","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Xi\u2019an People\u2019s Hospital (Xi\u2019an Fourth Hospital)","place":["Xi\u2019an, Shaanxi, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Xi\u2019an People\u2019s Hospital (Xi\u2019an Fourth Hospital)","place":["Xi\u2019an, Shaanxi, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"111826","DOI":"10.1016\/j.celrep.2022.111826","article-title":"Bcl-xL inhibition potentiates cancer therapies by redirecting the outcome of p53 activation from senescence to apoptosis","volume":"41","author":"Bharti","year":"2022","journal-title":"Cell. 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