{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:01:06Z","timestamp":1784689266099,"version":"3.55.0"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Lymph node metastasis (LNM) is a key prognostic determinant in thyroid carcinoma (THCA), yet molecular markers capturing intrinsic metastatic potential are limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Generalized additive models were applied to TCGA-THCA data to screen for genes with diametrically opposite expression\u2013tumor diameter relationships between N0 and N1 patients. CENPM was subsequently validated in independent transcriptomic cohorts, spatial transcriptomics, and immunohistochemistry. Single-cell transcriptomics,\n                      <jats:italic>in silico<\/jats:italic>\n                      knockout, drug repositioning, and molecular docking were employed to dissect its immunological roles and therapeutic relevance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CENPM expression increased with tumor diameter in N0 but decreased in N1, and high CENPM was associated with poorer disease-free survival. CENPM was predominantly enriched in CD8\n                      <jats:sup>+<\/jats:sup>\n                      na\u00efve and effector T cells, particularly in anaplastic carcinoma. Virtual knockout predicted downstream transcriptional changes associated with lymphocyte activation, translational machinery, and immune effector pathways. Drug repositioning identified filgotinib as a candidate to reverse the CENPM-high signature, with stable CENPM\u2013filgotinib binding confirmed by docking and molecular dynamics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CENPM shows diametrically opposite expression\u2013diameter relationships between N0 and N1 patients, suggesting a shift in biological behavior upon nodal involvement, with potential therapeutic relevance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fgene.2026.1875148","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T02:02:51Z","timestamp":1784685771000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["CENPM as a biomarker and therapeutic target for lymph node metastasis in thyroid carcinoma"],"prefix":"10.3389","volume":"17","author":[{"given":"Ligong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical University","place":["Bengbu, Anhui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical University","place":["Bengbu, Anhui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, The First Affiliated Hospital of Bengbu Medical University","place":["Bengbu, Anhui, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"10376","DOI":"10.1038\/s41467-024-54794-x","article-title":"Cullin-5 deficiency promotes chimeric antigen receptor T cell effector functions potentially via the modulation of JAK\/STAT signaling pathway","volume":"15","author":"Adachi","year":"2024","journal-title":"Nat. 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