{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:18:16Z","timestamp":1788387496593,"version":"build-2803163510"},"reference-count":42,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:p>Thyroid cancer is the most common type of endocrine malignancy, and its aggressive types are diagnosed at advanced stage due to limited treatment options. This study aimed to identify upregulated SLC7A5 across thyroid cancer cell types using an integrated transcriptomics approach. Total RNA was extracted from different samples. Differential expression analysis was performed through DESeq2. Functional enrichment analyses were performed to explore key pathways. The PPI network was built using the STRING database to investigate the functional relationships among significantly differentially expressed genes. Differential expression analysis revealed that SLC7A5 was significantly upregulated in KTC-1. GO and KEGG analyses were enriched with cell adhesion, protein binding, extracellular exosome, RNA processing, ECM-receptor interactions, and focal adhesion. The PPI network analysis showed the interaction of SLC7A5 with TERF1, CARD10, PSAT1, SIRPA, and MYC. Western blot analysis revealed that expression of mTOR was not elevated in KTC-1. Overall, these results could provide valuable insights for further validation in thyroid cancer therapy.<\/jats:p>","DOI":"10.3389\/fgene.2026.1890433","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:10:30Z","timestamp":1788387030000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated transcriptomics approach identifies the upregulated SLC7A5, and associated pathways across different thyroid cancer cell types"],"prefix":"10.3389","volume":"17","author":[{"given":"Muhammad","family":"Naeem","sequence":"first","affiliation":[{"name":"Department of Breast and Thyroid Surgery, South China Hospital, Medical School, Shenzhen University","place":["Shenzhen, China"]},{"name":"Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, School of Biomedical Engineering, Shenzhen University, Medical School","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, South China Hospital, Medical School, Shenzhen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, South China Hospital, Medical School, Shenzhen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahid","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Centre for Applied Molecular Biology, University of the Punjab","place":["Lahore, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, South China Hospital, Medical School, Shenzhen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbin","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, South China Hospital, Medical School, Shenzhen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijian","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, South China Hospital, Medical School, Shenzhen University","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1089\/thy.2024.0266","article-title":"Transcriptome of anaplastic thyroid cancer reveals two molecular subtypes with distinct tumor microenvironment and prognosis","volume":"35","author":"Byun","year":"2025","journal-title":"Thyroid"},{"key":"B2","doi-asserted-by":"publisher","first-page":"2492","DOI":"10.1038\/s41388-022-02273-2","article-title":"STAT5A modulates CDYL2\/SLC7A6 pathway to inhibit the proliferation and invasion of hepatocellular carcinoma by targeting to mTORC1","volume":"41","author":"Chen","year":"2022","journal-title":"Oncogene"},{"key":"B3","doi-asserted-by":"publisher","first-page":"712","DOI":"10.3390\/biomedicines12040712","article-title":"Molecular pathology of thyroid tumors: essential points to comprehend regarding the latest WHO classification","volume":"12","author":"Chiba","year":"2024","journal-title":"Biomedicines"},{"key":"B4","doi-asserted-by":"publisher","first-page":"e48","DOI":"10.1016\/j.surge.2023.10.002","article-title":"Anaplastic thyroid cancer: improved understanding of what remains a deadly disease","volume":"22","author":"Cleere","year":"2024","journal-title":"Surg."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.3390\/jcm14051582","article-title":"Circulating biomarkers of thyroid cancer: an appraisal","volume":"14","author":"Codrich","year":"2025","journal-title":"J. Clin. Med."},{"key":"B6","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1007\/s00011-018-1154-7","article-title":"SCARF1: a multifaceted, yet largely understudied, scavenger receptor","volume":"67","author":"Patten","year":"2018","journal-title":"Inflamm. Res."},{"key":"B7","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1186\/s12935-021-01781-7","article-title":"SLC7A7 is a prognostic biomarker correlated with immune infiltrates in non-small cell lung cancer","volume":"21","author":"Dai","year":"2021","journal-title":"Cancer Cell Int."},{"key":"B8","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/s41389-023-00448-4","article-title":"Spindle function and Wnt pathway inhibition by PBX1 to suppress tumor progression via downregulating DCDC2 in colorectal cancer","volume":"12","author":"Dai","year":"2023","journal-title":"Oncogenesis"},{"key":"B9","doi-asserted-by":"publisher","first-page":"115130","DOI":"10.1016\/j.celrep.2024.115130","article-title":"SLC7A5 is required for cancer cell growth under arginine-limited conditions","volume":"44","author":"Dunlap","year":"2025","journal-title":"Cell Rep."},{"key":"B10","doi-asserted-by":"publisher","first-page":"e0251962","DOI":"10.1371\/journal.pone.0251962","article-title":"Integrated bioinformatics analysis and screening of hub genes in papillary thyroid carcinoma","volume":"16","author":"Fan","year":"2021","journal-title":"Plos One"},{"key":"B11","doi-asserted-by":"publisher","first-page":"3663","DOI":"10.3390\/ijms26083663","article-title":"The revolution of targeted therapies in thyroid cancer treatment: present and future promising anti-cancer drugs","volume":"26","author":"Gil-Bernab\u00e9","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"B12","doi-asserted-by":"publisher","first-page":"602","DOI":"10.21037\/tcr-20-2744","article-title":"Prognostic and clinical significance of Solute Carrier Family 7 Member 1 in ovarian cancer","volume":"10","author":"Gong","year":"2021","journal-title":"Transl. Cancer Res."},{"key":"B13","doi-asserted-by":"publisher","first-page":"131","DOI":"10.34172\/bma.26","article-title":"New developments in epigenetic and genetic biomarkers for differentiated thyroid cancer (DTC): moving toward accurate diagnosis and treatment by 2025","volume":"2","author":"Hosseinzadeh","year":"2025","journal-title":"Biomed. Adv."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.2533\/chimia.2022.1011","article-title":"Structure, function and pharmacology of SLC7 family members and homologues","volume":"76","author":"Jeckelmann","year":"2022","journal-title":"Chimia"},{"key":"B15","doi-asserted-by":"publisher","first-page":"209","DOI":"10.3390\/biology14020209","article-title":"The clinopathological and prognostic significance of SPOCK1 in gynecological cancers: a bioinformatics based analysis","volume":"14","author":"Karaman","year":"2025","journal-title":"Biology"},{"key":"B16","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1186\/s12885-026-15841-6","article-title":"Identification and validation of hub genes involved in papillary thyroid carcinoma progression","volume":"26","author":"Khalid","year":"2026","journal-title":"BMC Cancer"},{"key":"B17","doi-asserted-by":"publisher","first-page":"bqad118","DOI":"10.1210\/endocr\/bqad118","article-title":"Mechanistic insights of thyroid cancer progression","volume":"164","author":"Leandro-Garc\u00eda","year":"2023","journal-title":"Endocrinology"},{"key":"B18","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1016\/j.eprac.2026.02.005","article-title":"Clinical characteristics and outcomes of clinically detected versus imaging-detected incidental thyroid cancer","volume":"32","author":"Lee","year":"2026","journal-title":"Endocr. Pract."},{"key":"B19","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1016\/s2213-8587(25)00289-x","article-title":"Thyroid cancer in adolescents and young adults: a population-based study in 185 countries worldwide","volume":"14","author":"Li","year":"2026","journal-title":"Lancet Diabetes & Endocrinol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"101065","DOI":"10.1016\/j.jpha.2024.101065","article-title":"A gene set enrichment analysis for cancer hallmarks","volume":"15","author":"Menyhart","year":"2025","journal-title":"J. Pharm. Analysis"},{"key":"B21","doi-asserted-by":"publisher","first-page":"e0329407","DOI":"10.1371\/journal.pone.0329407","article-title":"Prevalence and diagnostic reliability of BRAF, RAS mutations, and RET\/PTC rearrangements in a Latin American public health service population with thyroid nodular disease","volume":"20","author":"Moposita Molina","year":"2025","journal-title":"PLoS One"},{"key":"B22","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/s13256-021-03249-8","article-title":"Anaplastic thyroid carcinoma with unusual long-term survival: a case report","volume":"16","author":"Moreno","year":"2022","journal-title":"J. Med. Case Rep."},{"key":"B23","doi-asserted-by":"publisher","first-page":"624324","DOI":"10.3389\/fimmu.2021.624324","article-title":"Targeting SLC1A5 and SLC3A2\/SLC7A5 as a potential strategy to strengthen anti-tumor immunity in the tumor microenvironment","volume":"12","author":"Nachef","year":"2021","journal-title":"Front. Immunology"},{"key":"B24","doi-asserted-by":"publisher","first-page":"103366","DOI":"10.1016\/j.arcmed.2025.103366","article-title":"Decoding the mutational hierarchy of thyroid cancer and associated signaling pathways","volume":"57","author":"Nashier","year":"2026","journal-title":"Archives Med. Res."},{"key":"B25","doi-asserted-by":"publisher","first-page":"e27928","DOI":"10.1016\/j.heliyon.2024.e27928","article-title":"Integrated transcriptome sequencing and weighted gene co-expression network analysis reveals key genes of papillary thyroid carcinomas","volume":"10","author":"Pan","year":"2024","journal-title":"Heliyon"},{"key":"B26","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.yexmp.2015.12.011","article-title":"RNA sequencing identifies crucial genes in papillary thyroid carcinoma (PTC) progression","volume":"100","author":"Qiu","year":"2016","journal-title":"Exp. Molecular Pathology"},{"key":"B27","doi-asserted-by":"publisher","first-page":"152","DOI":"10.4103\/jhnps.jhnps_37_22","article-title":"Diagnostic role of Galectin-3 immunohistochemistry in thyroid lesions","volume":"10","author":"Sarin","year":"2022","journal-title":"J. Head & Neck Physicians Surg."},{"key":"B28","doi-asserted-by":"publisher","first-page":"371668","DOI":"10.3389\/fchem.2018.00243","article-title":"The human SLC7A5 (LAT1): the intriguing histidine\/large neutral amino acid transporter and its relevance to human health","volume":"6","author":"Scalise","year":"2018","journal-title":"Front. Chemistry"},{"key":"B29","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.3390\/cancers15061665","article-title":"Clinical implications of mTOR expression in papillary thyroid cancer\u2014A systematic review","volume":"15","author":"Derwich","year":"2023","journal-title":"Cancers"},{"key":"B30","doi-asserted-by":"publisher","first-page":"9710421","DOI":"10.1155\/2020\/9710421","article-title":"Identification of potential biomarkers for thyroid cancer using bioinformatics strategy: a study based on GEO datasets","volume":"2020","author":"Shen","year":"2020","journal-title":"BioMed Research International"},{"key":"B31","doi-asserted-by":"publisher","first-page":"3003","DOI":"10.1093\/hmg\/ddaa186","article-title":"The amino acid transporter Slc7a5 regulates the mTOR pathway and is required for granule cell development","volume":"29","author":"Sokolov","year":"2020","journal-title":"Hum. Molecular Genetics"},{"key":"B32","doi-asserted-by":"publisher","first-page":"629","DOI":"10.21037\/gs-20-673","article-title":"Identification of differentially expressed genes and signaling pathways in papillary thyroid cancer: a study based on integrated microarray and bioinformatics analysis","volume":"10","author":"Sun","year":"2021","journal-title":"Gland. Surg."},{"key":"B33","doi-asserted-by":"publisher","first-page":"D730","DOI":"10.1093\/nar\/gkae1113","article-title":"The STRING database in 2025: protein networks with directionality of regulation","volume":"53","author":"Szklarczyk","year":"2025","journal-title":"Nucleic Acids Research"},{"key":"B34","doi-asserted-by":"publisher","first-page":"168","DOI":"10.3390\/biomedicines14010168","article-title":"State of the art on thyroid cancer biology and oncology","volume":"14","author":"Vaio","year":"2026","journal-title":"Biomedicines"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1089\/dna.2021.0216","article-title":"The knockdown of ETV4 inhibits the papillary thyroid cancer development by promoting ferroptosis upon SLC7A11 downregulation","volume":"40","author":"Wang","year":"2021","journal-title":"Dna Cell Biology"},{"key":"B36","doi-asserted-by":"publisher","first-page":"30755","DOI":"10.1038\/s41598-024-80885-2","article-title":"N7-methyladenosine-induced SLC7A7 serves as a prognostic biomarker in pan-cancer and promotes CRC progression in colorectal cancer","volume":"14","author":"Wang","year":"2024","journal-title":"Sci. Rep."},{"key":"B37","doi-asserted-by":"publisher","first-page":"20977","DOI":"10.1038\/s41598-024-72020-y","article-title":"New insights into the mechanisms of the extracellular matrix and its therapeutic potential in anaplastic thyroid carcinoma","volume":"14","author":"Xia","year":"2024","journal-title":"Sci. Rep."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.18632\/aging.202231","article-title":"SPOCK1\/SIX1axis promotes breast cancer progression by activating AKT\/mTOR signaling","volume":"13","author":"Xu","year":"2020","journal-title":"Aging (Albany NY)"},{"key":"B39","doi-asserted-by":"publisher","first-page":"1467057","DOI":"10.3389\/fnut.2025.1467057","article-title":"SLC7 transporters at the crossroads of amino acid metabolism and diabetes pathophysiology: insights and therapeutic perspectives","volume":"12","author":"Xu","year":"","journal-title":"Front. Nutr."},{"key":"B40","doi-asserted-by":"publisher","first-page":"e25002","DOI":"10.53388\/2025825002","article-title":"Abnormal activation of the Ras\/MAPK signaling pathway in oncogenesis and progression","volume":"8","author":"Xu","year":"","journal-title":"Cancer Adv."},{"key":"B41","doi-asserted-by":"publisher","first-page":"BSR20201555","DOI":"10.1042\/BSR20201555","article-title":"Identification of key genes of papillary thyroid carcinoma by integrated bioinformatics analysis","volume":"40","author":"Xue","year":"2020","journal-title":"Biosci. Reports"},{"key":"B42","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.gene.2018.03.096","article-title":"Network-based meta-analysis in the identification of biomarkers for papillary thyroid cancer","volume":"661","author":"Zhao","year":"2018","journal-title":"Gene"}],"container-title":["Frontiers in Genetics"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fgene.2026.1890433\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:10:32Z","timestamp":1788387032000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fgene.2026.1890433\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":42,"alternative-id":["10.3389\/fgene.2026.1890433"],"URL":"https:\/\/doi.org\/10.3389\/fgene.2026.1890433","relation":{},"ISSN":["1664-8021"],"issn-type":[{"value":"1664-8021","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]},"article-number":"1890433"}}