{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:02:09Z","timestamp":1790031729517,"version":"4.0.1"},"reference-count":21,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Genet."],"abstract":"<jats:sec>\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>Ferroptosis-related pathways have been implicated in cardiovascular remodeling and cancer, but their relevance to hypertensive heart disease (HHD) remains uncertain. This study examined transcriptomic patterns in a dilated cardiomyopathy (DCM) dataset and used pan-cancer data only for descriptive context; it did not analyze primary HHD tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>GSE183852 data were restricted to the explicitly identified non-diseased sample H_ZC-11-292 (8,749 cells) and DCM sample HDCM1 (7,202 cells). H_ZC-LVAD-1 was excluded because GEO metadata classify it as DCM. Full-rank cell-level Wilcoxon results were retained, with all single-cell tests treated as descriptive. GSE5406 groups were assigned from GEO sample titles (194 failing and 16 non-failing myocardium samples), and TCGA data were used for descriptive pan-cancer context. GPX4, SLC7A11, ACSL4, and TFRC mRNA were assessed by RT-qPCR in Ang II-stimulated H9c2 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fourteen cluster-derived cell categories were retained. Cardiomyocytes, macrophages, and endothelial cells were not detected in the selected DCM donor, so no condition-specific inference was made for those categories. Among 109 detected ferroptosis-related genes, 60 met the cell-level up threshold, 24 met the down threshold, and 25 were nonsignificant; these counts are not donor-level evidence. In GSE5406, 5 of 99 tested ferroptosis-related genes met |log2FC| &amp;gt; 0.5 and BH-adjusted p &amp;lt; 0.05. Fold changes for 96 detected genes were uncorrelated across platforms (r = 0.034, p = 0.740). RT-qPCR showed lower GPX4 and SLC7A11 and higher ACSL4 and TFRC mRNA, with gene- and context-specific directionality (Supplementary Table S1).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The analyses identify exploratory, donor- and context-dependent transcriptional patterns. They do not establish ferroptosis as a causal mechanism, biomarker, or treatment target in primary HHD. Multi-donor disease-specific cohorts and protein-level and functional experiments are required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fgene.2026.1909716","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:10:19Z","timestamp":1790028619000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploratory ferroptosis-related transcriptomic patterns in dilated cardiomyopathy and pan-cancer datasets with H9c2 RT-qPCR assessment"],"prefix":"10.3389","volume":"17","author":[{"given":"Mingwei","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Cardiology, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huayin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangchao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boli","family":"Ran","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"He","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Chongqing General Hospital, Chongqing University","place":["Chongqing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: tool for the unification of biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. 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