{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T17:03:12Z","timestamp":1781802192307,"version":"3.54.5"},"reference-count":21,"publisher":"Frontiers Media SA","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2014.00444","type":"journal-article","created":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T14:57:24Z","timestamp":1410965844000},"source":"Crossref","is-referenced-by-count":3,"title":["The Role of the Lys628 (192) Residue of the Complement Protease, C1s, in Interacting with Peptide and Protein Substrates"],"prefix":"10.3389","volume":"5","author":[{"given":"Lakshmi Carmel","family":"Wijeyewickrema","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee Charlene","family":"Duncan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Neil","family":"Pike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2014,9,17]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1038\/ni1113","article-title":"The complement system in regulation of adaptive immunity","volume":"5","author":"Carroll","year":"2004","journal-title":"Nat Immunol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1038\/ni.1923","article-title":"Complement: a key system for immune surveillance and homeostasis","volume":"11","author":"Ricklin","year":"2010","journal-title":"Nat Immunol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/S1471-4906(01)02129-9","article-title":"Complement in inflammatory tissue damage and disease","volume":"23","author":"Mollnes","year":"2002","journal-title":"Trends Immunol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.biochi.2007.07.023","article-title":"The initiating proteases of the complement system: controlling the cleavage","volume":"90","author":"Duncan","year":"2008","journal-title":"Biochimie"},{"key":"B5","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.sbi.2012.04.001","article-title":"The modular serine proteases of the complement cascade","volume":"22","author":"Forneris","year":"2012","journal-title":"Curr Opin Struct Biol"},{"key":"B6","doi-asserted-by":"publisher","first-page":"15821","DOI":"10.1074\/jbc.M113.464545","article-title":"A molecular switch governs the interaction between the human complement protease C1s and its substrate, complement C4","volume":"288","author":"Perry","year":"2013","journal-title":"J Biol Chem"},{"key":"B7","doi-asserted-by":"publisher","first-page":"2365","DOI":"10.4049\/jimmunol.1201085","article-title":"Identification of a catalytic exosite for complement component C4 on the serine protease domain of C1s","volume":"189","author":"Duncan","year":"2012","journal-title":"J Immunol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"15425","DOI":"10.1073\/pnas.1208031109","article-title":"Structural basis for activation of the complement system by component C4 cleavage","volume":"109","author":"Kidmose","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1374","DOI":"10.4049\/jimmunol.170.3.1374","article-title":"Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments","volume":"170","author":"Ambrus","year":"2003","journal-title":"J Immunol"},{"key":"B10","doi-asserted-by":"publisher","first-page":"41811","DOI":"10.1074\/jbc.M503813200","article-title":"Functional characterization of complement proteases c1s\/mannan-binding lectin-associated serine protease-2 (MASP-2) chimeras reveals the higher c4 recognition efficacy of the MASP-2 complement control protein modules","volume":"280","author":"Rossi","year":"2005","journal-title":"J Biol Chem"},{"key":"B11","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1016\/j.molimm.2011.10.006","article-title":"Multiple domains of MASP-2, an initiating complement protease, are required for interaction with its substrate C4","volume":"49","author":"Duncan","year":"2012","journal-title":"Mol Immunol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1755","DOI":"10.1093\/emboj\/19.8.1755","article-title":"Crystal structure of the catalytic domain of human complement C1s: a serine protease with a handle","volume":"19","author":"Gaboriaud","year":"2000","journal-title":"EMBO J"},{"key":"B13","doi-asserted-by":"publisher","first-page":"39510","DOI":"10.1074\/jbc.M506131200","article-title":"Elucidation of the substrate specificity of the C1s protease of the classical complement pathway","volume":"280","author":"Kerr","year":"2005","journal-title":"J Biol Chem"},{"key":"B14","doi-asserted-by":"publisher","first-page":"41077","DOI":"10.1074\/jbc.M506766200","article-title":"Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein","volume":"280","author":"Tang","year":"2005","journal-title":"J Biol Chem"},{"key":"B15","doi-asserted-by":"publisher","first-page":"7371","DOI":"10.1073\/pnas.88.16.7371","article-title":"Glu192 \u00e2\u2020\u2019 Gln substitution in thrombin mimics the catalytic switch induced by thrombomodulin","volume":"88","author":"Le Bonniec","year":"1991","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B16","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1111\/j.1432-1033.1996.477rr.x","article-title":"Molecular basis of residue 192 participation in determination of coagulation protease specificity","volume":"242","author":"Rezaie","year":"1996","journal-title":"Eur J Biochem"},{"key":"B17","doi-asserted-by":"publisher","first-page":"22399","DOI":"10.1074\/jbc.M113.483875","article-title":"The X-ray crystal structure of mannose-binding lectin-associated serine proteinase-3 reveals the structural basis for enzyme inactivity associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) syndrome","volume":"288","author":"Yongqing","year":"2013","journal-title":"J Biol Chem"},{"key":"B18","doi-asserted-by":"publisher","first-page":"14939","DOI":"10.1021\/bi035507b","article-title":"The importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates","volume":"42","author":"O\u00e2\u20ac\u2122Brien","year":"2003","journal-title":"Biochemistry"},{"key":"B19","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1111\/j.1742-4658.2010.07642.x","article-title":"Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2\u00e2\u20ac\u2122 of substrates","volume":"277","author":"Andersson","year":"2010","journal-title":"FEBS J."},{"key":"B20","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1074\/jbc.275.2.809","article-title":"The role of Glu192 in the allosteric control of the S2\u00e2\u20ac\u2122 and S3\u00e2\u20ac\u2122 subsites of thrombin","volume":"275","author":"Marque","year":"2000","journal-title":"J Biol Chem"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1533","DOI":"10.1016\/j.jmb.2004.07.014","article-title":"The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions","volume":"342","author":"Harmat","year":"2004","journal-title":"J Mol Biol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"deposited":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T20:35:57Z","timestamp":1498163757000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2014.00444\/abstract"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,17]]},"references-count":21,"URL":"https:\/\/doi.org\/10.3389\/fimmu.2014.00444","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,17]]}}}