{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:17:43Z","timestamp":1790183863817,"version":"4.1.0"},"reference-count":59,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1130093"],"award-info":[{"award-number":["1130093"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2017.00125","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T22:32:18Z","timestamp":1486593138000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":28,"title":["Immunogenicity of a Multi-Epitope DNA Vaccine Encoding Epitopes from Cu\u2013Zn Superoxide Dismutase and Open Reading Frames of Brucella abortus in Mice"],"prefix":"10.3389","volume":"8","author":[{"given":"Emilia","family":"Escalona","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darwin","family":"S\u00e1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"O\u00f1ate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2017,2,9]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.2174\/1574891X11308010004","article-title":"Microbiology of Brucella","volume":"8","author":"Percin","year":"2012","journal-title":"Recent Pat Antiinfect Drug Discov"},{"key":"B2","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1016\/j.vetmic.2009.06.021","article-title":"Brucellosis: a re-emerging zoonosis","volume":"140","author":"Seleem","year":"2010","journal-title":"Vet Microbiol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1146\/annurev-micro-090110-102905","article-title":"Interactions of the human pathogenic Brucella species with their hosts","volume":"65","author":"Atluri","year":"2011","journal-title":"Annu Rev Microbiol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1505","DOI":"10.1016\/j.ajpath.2015.03.003","article-title":"Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions","volume":"185","author":"de Figueiredo","year":"2015","journal-title":"Am J Pathol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"B6","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.tvjl.2009.04.010","article-title":"Pathogenesis of Bovine brucellosis","volume":"184","author":"Carvalho","year":"2010","journal-title":"Vet J"},{"key":"B7","doi-asserted-by":"publisher","first-page":"589494","DOI":"10.1155\/2012\/589494","article-title":"Host susceptibility to Brucella abortus infection is more pronounced in IFN-\u03b3 knockout than IL-12\/\u03b2 2-microglobulin double-deficient mice","volume":"2012","author":"Brand\u00e3o","year":"2012","journal-title":"Clin Dev Immunol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"2325","DOI":"10.1056\/NEJMra050570","article-title":"Brucellosis","volume":"352","author":"Pappas","year":"2005","journal-title":"N Engl J Med"},{"key":"B9","doi-asserted-by":"publisher","first-page":"124509","DOI":"10.1155\/2010\/124509","article-title":"Importance of lipopolysaccharide and cyclic \u03b2-1,2-glucans in Brucella-mammalian infections","volume":"2010","author":"Haag","year":"2010","journal-title":"Int J Microbiol"},{"key":"B10","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1084\/jem.20030088","article-title":"Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum","volume":"198","author":"Celli","year":"2003","journal-title":"J Exp Med"},{"issue":"2","key":"B11","doi-asserted-by":"publisher","first-page":"e21","DOI":"10.1371\/journal.ppat.0040021","article-title":"Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1","volume":"4","author":"Salcedo","year":"2008","journal-title":"PLoS Pathog"},{"key":"B12","doi-asserted-by":"publisher","first-page":"e10216","DOI":"10.1371\/journal.pone.0010216","article-title":"Transcriptome analysis of the Brucella abortus BvrR\/BvrS two-component regulatory system","volume":"5","author":"Viadas","year":"2010","journal-title":"PLoS One"},{"issue":"6","key":"B13","doi-asserted-by":"publisher","first-page":"1570","DOI":"10.3389\/fmicb.2015.01570","article-title":"Deletion of the small RNA chaperone protein Hfq down regulates genes related to virulence and confers protection against wild-type Brucella challenge in mice","volume":"20","author":"Lei","year":"2016","journal-title":"Front Microbiol"},{"key":"B14","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1021\/bi00454a010","article-title":"A protein isolated from Brucella abortus is a Cu-Zn superoxide dismutase","volume":"29","author":"Beck","year":"1990","journal-title":"Biochemistry"},{"key":"B15","doi-asserted-by":"publisher","first-page":"3834","DOI":"10.1128\/JB.00343-15","article-title":"Structural, functional, and immunogenic insights on Cu, Zn superoxide dismutase pathogenic virulence factors from Neisseria meningitidis and Brucella abortus","volume":"197","author":"Pratt","year":"2015","journal-title":"J Bacteriol"},{"key":"B16","doi-asserted-by":"publisher","first-page":"4857","DOI":"10.1128\/IAI.71.9.4857-4861.2003","article-title":"A DNA vaccine encoding Cu, Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB\/c mice","volume":"71","author":"O\u00f1ate","year":"2003","journal-title":"Infect Immun"},{"key":"B17","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1128\/IAI.72.4.2081-2087.2004","article-title":"Intraspleen delivery of a DNA vaccine coding for superoxide dismutase (SOD) of Brucella abortus induces SOD-specific CD4+ and CD8+ T cells","volume":"72","author":"Mu\u00f1oz-Montesino","year":"2004","journal-title":"Infect Immun"},{"key":"B18","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1128\/JCM.39.1.235-240.2001","article-title":"Limited genetic diversity of Brucella spp","volume":"39","author":"G\u00e1ndara","year":"2001","journal-title":"J Clin Microbiol"},{"issue":"15","key":"B19","doi-asserted-by":"publisher","first-page":"5040","DOI":"10.1128\/JB.186.15.5040-5051.2004","article-title":"Comparative whole-genome hybridization reveals genomic islands in Brucella species","volume":"186","author":"Rajashekara","year":"2004","journal-title":"J Bacteriol"},{"issue":"6","key":"B20","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/1471-2180-6-13","article-title":"Molecular targets for rapid identification of Brucella spp","volume":"22","author":"Ratushna","year":"2006","journal-title":"BMC Microbiol"},{"key":"B21","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.vetmic.2014.05.005","article-title":"Roles of genomic island 3 (GI-3) BAB1_0267 and BAB1_0270 open reading frames (ORFs) in the virulence of Brucella abortus 2308","volume":"172","author":"Ortiz-Rom\u00e1n","year":"2014","journal-title":"Vet Microbiol"},{"key":"B22","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.vetmic.2011.09.025","article-title":"Roles of genomic island 3 (IG3) BAB1_0278 and BAB1_0263 open reading frames (ORFs) in the virulence of Brucella abortus in BALB\/c mice","volume":"156","author":"C\u00e9spedes","year":"2012","journal-title":"Vet Microbiol"},{"key":"B23","doi-asserted-by":"publisher","first-page":"117","DOI":"10.3389\/fcimb.2016.00117","article-title":"Immunogenicity and protective response induced by recombinant plasmids based on the BAB1_0267 and BAB1_0270 open reading frames of Brucella abortus 2308 in BALB\/c mice","volume":"6","author":"G\u00f3mez","year":"2016","journal-title":"Front Cell Infect Microbiol"},{"key":"B24","doi-asserted-by":"publisher","first-page":"7286","DOI":"10.1016\/j.vaccine.2012.09.039","article-title":"Evaluation of protective effect of DNA vaccines encoding the BAB1_0263 and BAB1_0278 open reading frames of Brucella abortus in BALB\/c mice","volume":"30","author":"Sislema-Egas","year":"2012","journal-title":"Vaccine"},{"key":"B25","doi-asserted-by":"publisher","first-page":"3663","DOI":"10.1016\/j.vaccine.2013.06.013","article-title":"Protective effect of a DNA vaccine containing an open reading frame with homology to an ABC-type transporter present in the genomic island 3 of Brucella abortus in BALB\/c mice","volume":"31","author":"Riquelme-Neira","year":"2013","journal-title":"Vaccine"},{"key":"B26","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.vetmic.2012.07.016","article-title":"Vaccination with recombinant flagellar proteins FlgJ and FliN induce protection against Brucella abortus 544 infection in BALB\/c mice","volume":"161","author":"Li","year":"2012","journal-title":"Vet Microbiol"},{"issue":"9","key":"B27","doi-asserted-by":"publisher","first-page":"e0136696","DOI":"10.1371\/journal.pone.0136696","article-title":"Immune response of calves vaccinated with Brucella abortus S19 or RB51 and revaccinated with RB51","volume":"10","author":"Dorneles","year":"2015","journal-title":"PLoS One"},{"key":"B28","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet Microbiol"},{"key":"B29","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1586\/erv.10.57","article-title":"DNA vaccines for targeting bacterial infections","volume":"9","author":"Ingolotti","year":"2010","journal-title":"Expert Rev Vaccines"},{"key":"B30","doi-asserted-by":"publisher","first-page":"208","DOI":"10.4161\/hv.18389","article-title":"Immunogenicity of a multi-epitope DNA vaccine against hantavirus","volume":"8","author":"Zhao","year":"2012","journal-title":"Hum Vaccin Immuniother"},{"key":"B31","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1111\/1348-0421.12410","article-title":"DNA vaccine with discontinuous T-cell epitope insertions into HSP65 scaffold as a potential means to improve immunogenicity of multi-epitope Mycobacterium tuberculosis vaccine","volume":"60","author":"Wu","year":"2016","journal-title":"Microbiol Imunol"},{"key":"B32","doi-asserted-by":"publisher","first-page":"3751","DOI":"10.1016\/j.vaccine.2016.06.004","article-title":"Evaluation of a chimeric multi-epitope-based DNA vaccine against subgroup J avian leukosis virus in chickens","volume":"34","author":"Xu","year":"2016","journal-title":"Vaccine"},{"key":"B33","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1111\/j.1365-2567.2012.03611.x","article-title":"The immune epitope database: a historical retrospective of the first decade","volume":"137","author":"Salimi","year":"2012","journal-title":"Immunology"},{"key":"B34","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1186\/1471-2105-6-132","article-title":"Generating quantitative models describing the sequence specificity of biological processes with the stabilized matrix method","volume":"6","author":"Peters","year":"2005","journal-title":"BMC Bioinformatics"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1110\/ps.0239403","article-title":"Reliable prediction of T-cell epitopes using neural networks with novel sequence representations","volume":"12","author":"Nielsen","year":"2003","journal-title":"Protein Sci"},{"key":"B36","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/1745-7580-4-2","article-title":"Quantitative peptide binding motifs for 19 human and mouse MHC class I molecules derived using positional scanning combinatorial peptide libraries","volume":"4","author":"Sidney","year":"2008","journal-title":"Immunome Res"},{"key":"B37","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1046\/j.1439-0450.2000.00402.x","article-title":"Frequent exposure of mice to crude Brucella abortus proteins down-regulates immune response","volume":"47","author":"O\u00f1ate","year":"2000","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"B38","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.imbio.2008.07.004","article-title":"Cloning, expression and immunogenicity of the translation initiation factor 3 homologue of Brucella abortus","volume":"214","author":"Gonz\u00e1lez","year":"2009","journal-title":"Immunobiol"},{"key":"B39","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1016\/0264-410X(94)90035-3","article-title":"Modulation of immune responses in Balb\/c mice vaccinated with Brucella abortus Cu-Zn superoxide dismutase synthetic peptide vaccine","volume":"12","author":"Tabatabai","year":"1994","journal-title":"Vaccine"},{"key":"B40","doi-asserted-by":"publisher","first-page":"5499","DOI":"10.4049\/jimmunol.168.11.5499","article-title":"A rational strategy to design multiepitope immunogens based on multiple Th lymphocyte epitopes","volume":"168","author":"Livingston","year":"2002","journal-title":"J Immunol"},{"key":"B41","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/S0378-1135(02)00252-3","article-title":"Epidemiology and control of brucellosis in China","volume":"90","author":"Deqiu","year":"2002","journal-title":"Vet Microbiol"},{"key":"B42","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/S1369-5274(00)00119-3","article-title":"Reverse vaccinology","volume":"3","author":"Rappuoli","year":"2000","journal-title":"Curr Opin Microbiol"},{"key":"B43","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1126\/science.aab3886","article-title":"Protective eficacy of adenovirus\/protein vaccines a gainst SIV challenges in rhesus monkeys","volume":"349","author":"Barouch","year":"2015","journal-title":"Science"},{"key":"B44","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1111\/j.1365-3024.2003.00651.x","article-title":"A DNA-prime\/protein-boost vaccination regimen enhances Th2 immune responses but not protection following Schistosoma mansoni infection","volume":"25","author":"Da\u2019Dara","year":"2003","journal-title":"Parasite Immunol"},{"key":"B45","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1146\/annurev.immunol.18.1.927","article-title":"DNA vaccines: immunology, application, and optimization","volume":"18","author":"Gurunathan","year":"2000","journal-title":"Ann Rev Immunol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"915","DOI":"10.4049\/jimmunol.165.2.915","article-title":"Requirements for the maintenance of Th1 immunity in vivo following DNA vaccination: a potential immunoregulatory role for CD8+ T cells","volume":"165","author":"Gurunathan","year":"2000","journal-title":"J Immunol"},{"key":"B47","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1111\/j.1365-3024.2008.01025.x","article-title":"Protective effect of a multiantigenic DNA vaccine against Toxoplasma gondii with co-delivery of IL-12 in mice","volume":"30","author":"Cui","year":"2008","journal-title":"Parasite Immunol"},{"key":"B48","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.vetpar.2009.08.016","article-title":"Induction of protective immunity by multiantigenic DNA vaccine delivered in attenuated Salmonella typhimurium against Toxoplasma gondii infection in mice","volume":"166","author":"Qu","year":"2009","journal-title":"Vet Parasitol"},{"key":"B49","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1089\/dna.2006.0552","article-title":"A combined DNA vaccine encoding BCSP31, SOD, and L7\/L12 confers high protection against Brucella abortus 2308 by inducing specific CTL responses","volume":"26","author":"Yu","year":"2007","journal-title":"DNA Cell Biol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1016\/S0952-7915(03)00083-9","article-title":"Epitope-based vaccines: an update on epitope identification, vaccine design and delivery","volume":"15","author":"Sette","year":"2003","journal-title":"Curr Opin Immunol"},{"key":"B51","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1128\/JVI.01505-07","article-title":"Rational design of a multiepitope vaccine encoding T-lymphocyte epitopes for treatment of chronic hepatitis B virus infections","volume":"82","author":"Depla","year":"2008","journal-title":"J Virol"},{"key":"B52","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.bbrc.2008.09.125","article-title":"The immunoreactivity of a chimeric multi-epitope DNA vaccine against IBV in chickens","volume":"377","author":"Tian","year":"2008","journal-title":"Biochem Biophys Res Commun"},{"key":"B53","doi-asserted-by":"publisher","first-page":"2085","DOI":"10.1016\/j.vaccine.2010.12.130","article-title":"HelicoVax: epitope-based therapeutic Helicobacter pylori vaccination in a mouse model","volume":"29","author":"Moss","year":"2011","journal-title":"Vaccine"},{"key":"B54","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.jtbi.2014.01.018","article-title":"A novel multi-epitope peptide vaccine against cancer: an in silico approach","volume":"349","author":"Nezafat","year":"2014","journal-title":"J Theor Biol"},{"key":"B55","doi-asserted-by":"publisher","first-page":"1110","DOI":"10.2174\/1389201017666160914191106","article-title":"Harnessing bioinformatics for designing a novel multi-epitope peptide vaccine against breast cancer","volume":"17","author":"Mahmoodi","year":"2016","journal-title":"Curr Pharm Biotechnol"},{"key":"B56","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1111\/j.1574-6976.2008.00109.x","article-title":"Chemical gene synthesis: strategies, softwares, error corrections, and applications","volume":"32","author":"Xiong","year":"2008","journal-title":"FEMS Microbiol Rev"},{"key":"B57","first-page":"75","article-title":"SYFPEITHI, database for searching and T-cell epitope prediction","volume-title":"Immunoinformatics. Predicting Immunogenicity in silico","author":"Schuler","year":"2007"},{"key":"B58","doi-asserted-by":"publisher","first-page":"D405","DOI":"10.1093\/nar\/gku938","article-title":"The immune epitope database (IEDB) 3.0","volume":"43","author":"Vita","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"B59","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/S1286-4579(00)01350-2","article-title":"Immunity and protection against Brucella abortus","volume":"3","author":"Golding","year":"2001","journal-title":"Microbes Infect"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2017.00125\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T22:32:20Z","timestamp":1486593140000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2017.00125\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,9]]},"references-count":59,"URL":"https:\/\/doi.org\/10.3389\/fimmu.2017.00125","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,9]]}}}