{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:51:04Z","timestamp":1787068264158,"version":"build-2736575974"},"reference-count":63,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T00:00:00Z","timestamp":1516752000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2018.00047","type":"journal-article","created":{"date-parts":[[2018,1,23]],"date-time":"2018-01-23T23:16:45Z","timestamp":1516749405000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":12,"title":["Neutralizing Antibodies Induced by Gene-Based Hydrodynamic Injection Have a Therapeutic Effect in Lethal Influenza Infection"],"prefix":"10.3389","volume":"9","author":[{"given":"Tatsuya","family":"Yamazaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Nagashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Ninomiya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ainai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Fujimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Ichimonji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidekazu","family":"Takagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Morita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenta","family":"Murotani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Chiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Akashi-Takamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,1,24]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1128\/CMR.13.4.602-614.2000","article-title":"Passive immunity in prevention and treatment of infectious diseases","volume":"13","author":"Keller","year":"2000","journal-title":"Clin Microbiol Rev"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1421","DOI":"10.1038\/nbt1363","article-title":"The growth and potential of human antiviral monoclonal antibody therapeutics","volume":"25","author":"Marasco","year":"2007","journal-title":"Nat Biotechnol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.vaccine.2009.09.105","article-title":"Monoclonal antibody-based therapies for microbial diseases","volume":"27","author":"Saylor","year":"2009","journal-title":"Vaccine"},{"key":"B4","unstructured":"Influenza (Seasonal) Fact Sheet2016"},{"key":"B5","doi-asserted-by":"publisher","first-page":"2296","DOI":"10.1056\/NEJMc0910060","article-title":"Emergence of oseltamivir-resistant pandemic H1N1 virus during prophylaxis","volume":"361","author":"Baz","year":"2009","journal-title":"N Engl J Med"},{"key":"B6","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1038\/nature08260","article-title":"In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses","volume":"460","author":"Itoh","year":"2009","journal-title":"Nature"},{"key":"B7","doi-asserted-by":"publisher","first-page":"e1001079","DOI":"10.1371\/journal.ppat.1001079","article-title":"Characterization of oseltamivir-resistant 2009 H1N1 pandemic influenza A viruses","volume":"6","author":"Kiso","year":"2010","journal-title":"PLoS Pathog"},{"key":"B8","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.antiviral.2012.07.002","article-title":"Treatment of oseltamivir-resistant influenza A (H1N1) virus infections in mice with antiviral agents","volume":"96","author":"Smee","year":"2012","journal-title":"Antiviral Res"},{"key":"B9","doi-asserted-by":"crossref","first-page":"573","DOI":"10.18388\/abp.2014_1879","article-title":"Influenza prevention and treatment by passive immunization","volume":"61","author":"Kalenik","year":"2014","journal-title":"Acta Biochim Pol"},{"key":"B10","doi-asserted-by":"crossref","first-page":"40","DOI":"10.7883\/yoken.64.40","article-title":"Passive immune-prophylaxis against influenza virus infection by the expression of neutralizing anti-hemagglutinin monoclonal antibodies from plasmids","volume":"64","author":"Yamazaki","year":"2011","journal-title":"Jpn J Infect Dis"},{"key":"B11","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1002\/jgm.541","article-title":"Hydrodynamics-based procedure involves transient hyperpermeability in the hepatic cellular membrane: implication of a nonspecific process in efficient intracellular gene delivery","volume":"6","author":"Kobayashi","year":"2004","journal-title":"J Gene Med"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1038\/sj.gt.3300947","article-title":"Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA","volume":"6","author":"Liu","year":"1999","journal-title":"Gene Ther"},{"key":"B13","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.4049\/jimmunol.146.6.1972","article-title":"Passive transfer of local immunity to influenza virus infection by IgA antibody","volume":"146","author":"Renegar","year":"1991","journal-title":"J Immunol"},{"key":"B14","doi-asserted-by":"publisher","first-page":"1978","DOI":"10.4049\/jimmunol.173.3.1978","article-title":"Role of IgA versus IgG in the control of influenza viral infection in the murine respiratory tract","volume":"173","author":"Renegar","year":"2004","journal-title":"J Immunol"},{"key":"B15","doi-asserted-by":"publisher","first-page":"7793","DOI":"10.1128\/JVI.00979-13","article-title":"Recombinant IgA is sufficient to prevent influenza virus transmission in guinea pigs","volume":"87","author":"Seibert","year":"2013","journal-title":"J Virol"},{"key":"B16","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1046\/j.1365-3083.2000.00790.x","article-title":"Role of J chain in secretory immunoglobulin formation","volume":"52","author":"Johansen","year":"2000","journal-title":"Scand J Immunol"},{"key":"B17","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1007\/s00281-016-0565-1","article-title":"IgE and mast cells in host defense against parasites and venoms","volume":"38","author":"Mukai","year":"2016","journal-title":"Semin Immunopathol"},{"key":"B18","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1038\/ni.1748","article-title":"Immunoglobulin D enhances immune surveillance by activating antimicrobial, proinflammatory and B cell \u2013 stimulating programs in basophils","volume":"10","author":"America","year":"2009","journal-title":"Nat Immunol"},{"key":"B19","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/j.bbrc.2007.11.135","article-title":"Influenza PR8 HA-specific Fab fragments produced by phage display methods","volume":"366","author":"Asanuma","year":"2008","journal-title":"Biochem Biophys Res Commun"},{"key":"B20","doi-asserted-by":"crossref","first-page":"139","DOI":"10.3233\/HAB-2006-15404","article-title":"Renewal of EBV-hybridoma method: efficient generation of recombinant fully human neutralizing IgG antibodies specific for tetanus toxin by use of tetroma cells","volume":"15","author":"Ainai","year":"2006","journal-title":"Hum Antibodies"},{"key":"B21","doi-asserted-by":"publisher","first-page":"835","DOI":"10.1016\/0006-291X(83)91672-8","article-title":"Rapid purification of plasmid DNA by a single centrifugation in a two-step cesium chloride-ethidium bromide gradient","volume":"117","author":"Garger","year":"1983","journal-title":"Biochem Biophys Res Commun"},{"key":"B22","doi-asserted-by":"crossref","first-page":"981","DOI":"10.4049\/jimmunol.149.3.981","article-title":"Cross-protection against influenza virus infection afforded by trivalent inactivated vaccines inoculated intranasally with cholera toxin B subunit","volume":"149","author":"Tamura","year":"1992","journal-title":"J Immunol"},{"key":"B23","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1038\/nbt0998-867","article-title":"Gene transfer into muscle by electroporation in vivo","volume":"16","author":"Aihara","year":"1998","journal-title":"Nat Biotechnol"},{"key":"B24","doi-asserted-by":"crossref","first-page":"41","DOI":"10.7883\/yoken.63.41","article-title":"Novel ELISA using intracellularly biotinylated antigen for detection of antibody following DNA immunization","volume":"63","author":"Kimura","year":"2010","journal-title":"Jpn J Infect Dis"},{"key":"B25","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.vascn.2010.12.003","article-title":"Generation of a panel of monoclonal antibodies against atypical chemokine receptor CCX-CKR by DNA immunization","volume":"63","author":"Takatsuka","year":"2011","journal-title":"J Pharmacol Toxicol Methods"},{"key":"B26","doi-asserted-by":"publisher","first-page":"75","DOI":"10.3233\/HAB-2009-0205","article-title":"Production of human antibodies to native cytokine receptors using the genetic immunization of KM mice","volume":"18","author":"Fujimoto","year":"2009","journal-title":"Hum Antibodies"},{"key":"B27","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.jim.2011.11.007","article-title":"Enhancement of antibody responses to native G protein-coupled receptors using E. coli GroEL as a molecular adjuvant in DNA immunization","volume":"375","author":"Fujimoto","year":"2012","journal-title":"J Immunol Methods"},{"key":"B28","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.1038\/sj.gt.3301522","article-title":"Optimisation of electrotransfer of plasmid into skeletal muscle by pretreatment with hyaluronidase-increased expression with reduced muscle damage","volume":"8","author":"McMahon","year":"2001","journal-title":"Gene Ther"},{"key":"B29","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1002\/jmv","article-title":"Zymosan enhances the mucosal adjuvant activity of poly(I:C) in a nasal influenza vaccine","volume":"82","author":"Ainai","year":"2010","journal-title":"J Med Virol"},{"key":"B30","doi-asserted-by":"crossref","first-page":"2779","DOI":"10.1016\/S0264-410X(00)00087-6","article-title":"Protection against influenza virus infection in mice immunized by administration of hemagglutinin-expressing DNAs with electroporation","volume":"18","author":"Kadowaki","year":"2000","journal-title":"Vaccine"},{"key":"B31","doi-asserted-by":"publisher","first-page":"2930","DOI":"10.4049\/jimmunol.168.6.2930","article-title":"Protection against influenza virus infection in polymeric Ig receptor knockout mice immunized intranasally with adjuvant-combined vaccines","volume":"168","author":"Asahi","year":"2002","journal-title":"J Immunol"},{"key":"B32","doi-asserted-by":"publisher","first-page":"3214","DOI":"10.1016\/S0264-410X(00)00149-3","article-title":"Cross-protection against a lethal influenza virus infection by DNA vaccine to neuraminidase","volume":"18","author":"Chen","year":"2000","journal-title":"Vaccine"},{"key":"B33","doi-asserted-by":"crossref","first-page":"195","DOI":"10.7883\/yoken.JJID.2005.195","article-title":"Mechanisms of broad cross-protection provided by influenza virus infection and their application to vaccines","volume":"58","author":"Tamura","year":"2005","journal-title":"Jpn J Infect Dis"},{"key":"B34","doi-asserted-by":"publisher","first-page":"187ra72","DOI":"10.1126\/scitranslmed.3006299","article-title":"Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza","volume":"5","author":"Limberis","year":"2013","journal-title":"Sci Transl Med"},{"key":"B35","doi-asserted-by":"publisher","first-page":"647","DOI":"10.1038\/nbt.2618","article-title":"Broad protection against influenza infection by vectored immunoprophylaxis in mice","volume":"31","author":"Balazs","year":"2013","journal-title":"Nat Biotechnol"},{"key":"B36","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.1038\/sj.gt.3301229","article-title":"Long-term expression of human alpha1-antitrypsin gene in mouse liver achieved by intravenous administration of plasmid DNA using a hydrodynamics-based procedure","volume":"7","author":"Zhang","year":"2000","journal-title":"Gene Ther"},{"key":"B37","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1038\/sj.gt.3302210","article-title":"Hydroporation as the mechanism of hydrodynamic delivery","volume":"11","author":"Zhang","year":"2004","journal-title":"Gene Ther"},{"key":"B38","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1038\/nature10660","article-title":"Antibody-based protection against HIV infection by vectored immunoprophylaxis","volume":"481","author":"Balazs","year":"2011","journal-title":"Nature"},{"key":"B39","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.virol.2008.04.016","article-title":"Genetic delivery of an anti-RSV antibody to protect against pulmonary infection with RSV","volume":"378","author":"Skaricic","year":"2008","journal-title":"Virology"},{"key":"B40","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1038\/nm.3471","article-title":"Vectored immunoprophylaxis protects humanized mice from mucosal HIV transmission","volume":"20","author":"Balazs","year":"2014","journal-title":"Nat Med"},{"key":"B41","doi-asserted-by":"publisher","first-page":"12528","DOI":"10.1073\/pnas.1407362111","article-title":"Vectored antibody gene delivery protects against Plasmodium falciparum sporozoite challenge in mice","volume":"111","author":"Deal","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B42","unstructured":"Gene therapy\u2026against the flu? CohenJ Science2013"},{"key":"B43","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1038\/nrg1066","article-title":"Progress and problems with the use of viral vectors for gene therapy","volume":"4","author":"Thomas","year":"2003","journal-title":"Nat Rev Genet"},{"key":"B44","doi-asserted-by":"publisher","first-page":"65","DOI":"10.3390\/genes8020065","article-title":"Advances in non-viral DNA vectors for gene therapy","volume":"8","author":"Hardee","year":"2017","journal-title":"Genes (Basel)"},{"key":"B45","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/1476-8518-7-3","article-title":"Prospects for control of emerging infectious diseases with plasmid DNA vaccines","volume":"7","author":"Moss","year":"2009","journal-title":"J Immune Based Ther Vaccines"},{"key":"B46","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1128\/JVI.69.4.2075-2081.1995","article-title":"Virus-neutralizing antibodies of immunoglobulin G (IgG) but not of IgM or IgA isotypes can cure influenza virus pneumonia in SCID mice","volume":"69","author":"Palladino","year":"1995","journal-title":"J Virol"},{"key":"B47","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1111\/j.1600-065X.1997.tb01009.x","article-title":"Role of the B-cell response in recovery of mice from primary influenza virus infection","volume":"159","author":"Gerhard","year":"1997","journal-title":"Immunol Rev"},{"key":"B48","doi-asserted-by":"publisher","first-page":"6099","DOI":"10.1016\/j.vaccine.2015.05.106","article-title":"Seasonal split influenza vaccine induced IgE sensitization against influenza vaccine","volume":"33","author":"Nakayama","year":"2015","journal-title":"Vaccine"},{"key":"B49","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1111\/j.1365-3083.2005.01710.x","article-title":"Influenza specific serum IgE is present in non-allergic subjects","volume":"62","author":"Brokstad","year":"2005","journal-title":"Scand J Immunol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"6831","DOI":"10.4049\/jimmunol.173.11.6831","article-title":"The glycosylation of human serum IgD and IgE and the accessibility of identified oligomannose structures for interaction with mannan-binding lectin","volume":"173","author":"Arnold","year":"2004","journal-title":"J Immunol"},{"key":"B51","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/S0161-5890(99)00036-X","article-title":"N-glycosylation influences epitope expression and receptor binding structures in human IgE","volume":"36","author":"Bj\u00f6rklund","year":"1999","journal-title":"Mol Immunol"},{"key":"B52","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1016\/0264-410X(93)90309-L","article-title":"Protection of germ-free mice from infection by Helicobacter felis after active oral or passive IgA immunization","volume":"11","author":"Czinn","year":"1993","journal-title":"Vaccine"},{"key":"B53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0168-1702(92)90063-F","article-title":"Comparison of IgA versus IgG monoclonal antibodies for passive immunization of the murine respiratory tract","volume":"23","author":"Mazanec","year":"1992","journal-title":"Virus Res"},{"key":"B54","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.molimm.2015.09.005","article-title":"IgA as therapeutic antibody","volume":"68","author":"Leusen","year":"2015","journal-title":"Mol Immunol"},{"key":"B55","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1038\/mi.2011.37","article-title":"Regulation of the polymeric immunoglobulin receptor and IgA transport: new advances in environmental factors that stimulate pIgR expression and its role in mucosal immunity","volume":"4","author":"Johansen","year":"2011","journal-title":"Mucosal Immunol"},{"key":"B56","doi-asserted-by":"publisher","first-page":"e107203","DOI":"10.1371\/journal.pone.0107203","article-title":"Safety assessment of liver-targeted hydrodynamic gene delivery in dogs","volume":"9","author":"Kamimura","year":"2014","journal-title":"PLoS One"},{"key":"B57","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1038\/sj.cgt.7701119","article-title":"Minimally invasive and selective hydrodynamic gene therapy of liver segments in the pig and human","volume":"15","author":"Khorsandi","year":"2008","journal-title":"Cancer Gene Ther"},{"key":"B58","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1038\/sj.gt.3303079","article-title":"Hydrodynamic gene delivery to the pig liver via an isolated segment of the inferior vena cava","volume":"15","author":"Fabre","year":"2008","journal-title":"Gene Ther"},{"key":"B59","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1038\/sj.gt.3302873","article-title":"Pig liver gene therapy by noninvasive interventionist catheterism","volume":"14","author":"Alino","year":"2007","journal-title":"Gene Ther"},{"key":"B60","doi-asserted-by":"publisher","first-page":"1696","DOI":"10.1038\/sj.gt.3302833","article-title":"Naked plasmid DNA transfer to the porcine liver using rapid injection with large volume","volume":"13","author":"Yoshino","year":"2006","journal-title":"Gene Ther"},{"key":"B61","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1016\/j.ymthe.2004.05.004","article-title":"A facile nonviral method for delivering genes and siRNAs to skeletal muscle of mammalian limbs","volume":"10","author":"Hagstrom","year":"2004","journal-title":"Mol Ther"},{"key":"B62","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/S0065-2660(05)54004-5","article-title":"Hydrodynamic delivery","volume":"54","author":"AI-Dosari","year":"2005","journal-title":"Adv Genet"},{"key":"B63","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/s40794-017-0046-0","article-title":"Vectored immunoprophylaxis: an emerging adjunct to traditional vaccination","volume":"3","author":"Sanders","year":"2017","journal-title":"Trop Dis Travel Med Vaccines"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2018.00047\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,30]],"date-time":"2024-06-30T14:12:17Z","timestamp":1719756737000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2018.00047\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,24]]},"references-count":63,"alternative-id":["10.3389\/fimmu.2018.00047"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2018.00047","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,24]]},"article-number":"47"}}