{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T04:12:35Z","timestamp":1774066355129,"version":"3.50.1"},"reference-count":53,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,2,19]],"date-time":"2018-02-19T00:00:00Z","timestamp":1518998400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005677","name":"Jos\u00e9 Carreras Leuk\u00e4mie-Stiftung","doi-asserted-by":"publisher","award":["DJCLS R 15\/02"],"award-info":[{"award-number":["DJCLS R 15\/02"]}],"id":[{"id":"10.13039\/501100005677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004426","name":"Dr. Werner Jackst\u00e4dt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004426","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2018.00280","type":"journal-article","created":{"date-parts":[[2018,2,19]],"date-time":"2018-02-19T04:18:07Z","timestamp":1519013887000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Alloreactive T Cell Receptor Diversity against Structurally Similar or Dissimilar HLA-DP Antigens Assessed by Deep Sequencing"],"prefix":"10.3389","volume":"9","author":[{"given":"Esteban","family":"Arrieta-Bola\u00f1os","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro","family":"Crivello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maximilian","family":"Metzing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thuja","family":"Meurer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00fcberra","family":"Ahci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Rytlewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marissa","family":"Vignali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Yusko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"van Balen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter A.","family":"Horn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H. Frederik","family":"Falkenburg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Fleischhauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2018,2,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1007\/978-1-4615-9017-0_37","article-title":"The proportion of lymphocytes capable of recognizing strong transplantation antigens in vivo","volume":"29","author":"Ford","year":"1973","journal-title":"Adv Exp Med Biol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1097\/TP.0000000000001064","article-title":"A new window into the human alloresponse","volume":"100","author":"DeWolf","year":"2016","journal-title":"Transplantation"},{"key":"B3","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1038\/nri2200","article-title":"Specificity of T-cell alloreactivity","volume":"7","author":"Felix","year":"2007","journal-title":"Nat Rev Immunol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"E4792","DOI":"10.1073\/pnas.1700459114","article-title":"How an alloreactive T-cell receptor achieves peptide and MHC specificity","volume":"114","author":"Wang","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1818","DOI":"10.1056\/NEJM199012273232607","article-title":"Bone marrow-allograft rejection by T lymphocytes recognizing a single amino acid difference in HLA-B44","volume":"323","author":"Fleischhauer","year":"1990","journal-title":"N Engl J Med"},{"key":"B6","first-page":"137","article-title":"HLA-B44-directed cytotoxic T cells associated with acute graft-versus-host disease following unrelated bone marrow transplantation","volume":"14","author":"Keever","year":"1994","journal-title":"Bone Marrow Transplant"},{"key":"B7","doi-asserted-by":"publisher","first-page":"1868","DOI":"10.4049\/jimmunol.1102086","article-title":"A proposed algorithm predictive for cytotoxic T cell alloreactivity","volume":"188","author":"Joris","year":"2012","journal-title":"J Immunol"},{"key":"B8","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.trim.2013.08.006","article-title":"Translating in vitro prediction of cytotoxic T cell alloreactivity to hematopoietic stem cell transplantation outcome","volume":"30","author":"Joris","year":"2014","journal-title":"Transpl Immunol"},{"key":"B9","doi-asserted-by":"publisher","first-page":"1991","DOI":"10.1182\/blood-2010-05-284687","article-title":"Significantly higher frequencies of alloreactive CD4+ T cells responding to nonpermissive than to permissive HLA-DPB1 T-cell epitope disparities","volume":"116","author":"Sizzano","year":"2010","journal-title":"Blood"},{"key":"B10","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/S0198-8859(97)00091-8","article-title":"Alloresponses to HLA-DP detected in the primary MLR: correlation with a single amino acid difference","volume":"55","author":"Nicholson","year":"1997","journal-title":"Hum Immunol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/0198-8859(92)90072-U","article-title":"Crucial role of the third and fourth hypervariable regions of HLA-DPB1 allelic sequences in the mixed lymphocyte reaction","volume":"33","author":"Cesbron","year":"1992","journal-title":"Hum Immunol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1417","DOI":"10.1182\/blood-2003-04-1279","article-title":"A T-cell epitope encoded by a subset of HLA-DPB1 alleles determines nonpermissive mismatches for hematologic stem cell transplantation","volume":"103","author":"Zino","year":"2004","journal-title":"Blood"},{"key":"B13","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/j.bbmt.2014.10.017","article-title":"The impact of amino acid variability on alloreactivity defines a functional distance predictive of permissive HLA-DPB1 mismatches in hematopoietic stem cell transplantation","volume":"21","author":"Crivello","year":"2015","journal-title":"Biol Blood Marrow Transplant"},{"key":"B14","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/S1470-2045(12)70004-9","article-title":"Effect of T-cell-epitope matching at HLA-DPB1 in recipients of unrelated-donor haemopoietic-cell transplantation: a retrospective study","volume":"13","author":"Fleischhauer","year":"2012","journal-title":"Lancet Oncol"},{"key":"B15","doi-asserted-by":"publisher","first-page":"2596","DOI":"10.1182\/blood-2014-05-576041","article-title":"Nonpermissive HLA-DPB1 mismatch increases mortality after myeloablative unrelated allogeneic hematopoietic cell transplantation","volume":"124","author":"Pidala","year":"2014","journal-title":"Blood"},{"key":"B16","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1097\/TP.0000000000001654","article-title":"Thymic origins of T cell receptor alloreactivity","volume":"101","author":"Brzostek","year":"2017","journal-title":"Transplantation"},{"key":"B17","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.bbmt.2012.07.020","article-title":"Patient HLA-DP-specific CD4+ T cells from HLA-DPB1-mismatched donor lymphocyte infusion can induce graft-versus-leukemia reactivity in the presence or absence of graft-versus-host disease","volume":"19","author":"Rutten","year":"2013","journal-title":"Biol Blood Marrow Transplant"},{"key":"B18","doi-asserted-by":"publisher","first-page":"1282","DOI":"10.1016\/j.bbmt.2010.03.018","article-title":"HLA-DPB1 mismatching results in the generation of a full repertoire of HLA-DPB1-specific CD4+ T cell responses showing immunogenicity of all HLA-DPB1 alleles","volume":"16","author":"Rutten","year":"2010","journal-title":"Biol Blood Marrow Transplant"},{"key":"B19","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1111\/cei.12152","article-title":"Activation-induced CD137 is a fast assay for identification and multi-parameter flow cytometric analysis of alloreactive T cells","volume":"174","author":"Litjens","year":"2013","journal-title":"Clin Exp Immunol"},{"key":"B20","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.1182\/blood-2009-04-217604","article-title":"Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells","volume":"114","author":"Robins","year":"2009","journal-title":"Blood"},{"key":"B21","doi-asserted-by":"publisher","first-page":"2680","DOI":"10.1038\/ncomms3680","article-title":"Using synthetic templates to design an unbiased multiplex PCR assay","volume":"4","author":"Carlson","year":"2013","journal-title":"Nat Commun"},{"key":"B22","doi-asserted-by":"publisher","first-page":"D413","DOI":"10.1093\/nar\/gku1056","article-title":"IMGT(R), the international ImMunoGeneTics information system(R) 25 years on","volume":"43","author":"Lefranc","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"B23","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/0022-5193(66)90133-0","article-title":"Species-diversity and pattern-diversity in the study of ecological succession","volume":"10","author":"Pielou","year":"1966","journal-title":"J Theor Biol"},{"key":"B24","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1038\/nmeth.2375","article-title":"Predicting the molecular complexity of sequencing libraries","volume":"10","author":"Daley","year":"2013","journal-title":"Nat Methods"},{"key":"B25","doi-asserted-by":"publisher","first-page":"4517","DOI":"10.1128\/JVI.03474-14","article-title":"Dynamics of the cytotoxic T cell response to a model of acute viral infection","volume":"89","author":"DeWitt","year":"2015","journal-title":"J Virol"},{"key":"B26","first-page":"215","article-title":"Measuring of the dispersion and analysis of distribution patterns","volume":"2","author":"Morisita","year":"1959","journal-title":"Mem Fac Sci Kyushu Univ Ser E Biol"},{"key":"B27","volume-title":"R: A Language and Environment for Statistical Computing","year":"2017"},{"key":"B28","volume-title":"RStudio: Integrated Development for R","year":"2015"},{"key":"B29","doi-asserted-by":"publisher","first-page":"e111943","DOI":"10.1371\/journal.pone.0111943","article-title":"Defining the alloreactive T cell repertoire using high-throughput sequencing of mixed lymphocyte reaction culture","volume":"9","author":"Emerson","year":"2014","journal-title":"PLoS One"},{"key":"B30","doi-asserted-by":"publisher","first-page":"eaah3732","DOI":"10.1126\/sciimmunol.aah3732","article-title":"Bidirectional intragraft alloreactivity drives the repopulation of human intestinal allografts and correlates with clinical outcome","volume":"1","author":"Zuber","year":"2016","journal-title":"Sci Immunol"},{"key":"B31","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1007\/s00251-004-0735-5","article-title":"Characterization of natural peptide ligands from HLA-DP2: new insights into HLA-DP peptide-binding motifs","volume":"56","author":"Diaz","year":"2005","journal-title":"Immunogenetics"},{"key":"B32","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/0198-8859(95)00022-V","article-title":"The role of HLA-DP beta residue 69 in the definition of antibody-binding epitopes","volume":"43","author":"Arroyo","year":"1995","journal-title":"Hum Immunol"},{"key":"B33","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1111\/j.1399-0039.1998.tb03020.x","article-title":"HLA-DPbeta residue 69 plays a crucial role in allorecognition","volume":"52","author":"Diaz","year":"1998","journal-title":"Tissue Antigens"},{"key":"B34","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1093\/intimm\/dxg057","article-title":"Functional analysis of HLA-DP polymorphism: a crucial role for DPbeta residues 9, 11, 35, 55, 56, 69 and 84-87 in T cell allorecognition and peptide binding","volume":"15","author":"Diaz","year":"2003","journal-title":"Int Immunol"},{"key":"B35","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1046\/j.1399-0039.2003.00131.x","article-title":"Detailed analysis of the effects of Glu\/Lys beta69 human leukocyte antigen-DP polymorphism on peptide-binding specificity","volume":"62","author":"Berretta","year":"2003","journal-title":"Tissue Antigens"},{"key":"B36","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/j.humimm.2005.06.007","article-title":"Allogeneic MHC class I molecules with numerous sequence differences do not elicit a CTL response","volume":"66","author":"Heemskerk","year":"2005","journal-title":"Hum Immunol"},{"key":"B37","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/sj.bmt.1705721","article-title":"Highly diverged MHC class I mismatches are acceptable for haematopoietic stem cell transplantation","volume":"40","author":"Heemskerk","year":"2007","journal-title":"Bone Marrow Transplant"},{"key":"B38","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1016\/j.beha.2010.09.015","article-title":"How closely related is graft-vs-leukemia to donor\/recipient disparity?","volume":"23","author":"Weisdorf","year":"2010","journal-title":"Best Pract Res Clin Haematol"},{"key":"B39","doi-asserted-by":"publisher","first-page":"1894","DOI":"10.1016\/j.molonc.2015.10.014","article-title":"Allo-reactive T cells for the treatment of hematological malignancies","volume":"9","author":"Falkenburg","year":"2015","journal-title":"Mol Oncol"},{"key":"B40","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1053\/j.seminhematol.2016.01.010","article-title":"HLA mismatching as a strategy to reduce relapse after alternative donor transplantation","volume":"53","author":"Fleischhauer","year":"2016","journal-title":"Semin Hematol"},{"key":"B41","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1182\/blood.V98.4.1122","article-title":"Peripheral blood stem cell allograft rejection mediated by CD4(+) T lymphocytes recognizing a single mismatch at HLA-DP beta 1*0901","volume":"98","author":"Fleischhauer","year":"2001","journal-title":"Blood"},{"key":"B42","doi-asserted-by":"publisher","first-page":"1220","DOI":"10.1182\/blood-2005-08-3121","article-title":"HLA-DPB1 matching status has significant implications for recipients of unrelated donor stem cell transplants","volume":"107","author":"Shaw","year":"2006","journal-title":"Blood"},{"key":"B43","doi-asserted-by":"publisher","first-page":"1001","DOI":"10.1038\/sj.bmt.1704029","article-title":"The degree of matching at HLA-DPB1 predicts for acute graft-versus-host disease and disease relapse following haematopoietic stem cell transplantation","volume":"31","author":"Shaw","year":"2003","journal-title":"Bone Marrow Transplant"},{"key":"B44","doi-asserted-by":"publisher","first-page":"5039","DOI":"10.4049\/jimmunol.1003650","article-title":"Strength of TCR-peptide\/MHC interactions and in vivo T cell responses","volume":"186","author":"Corse","year":"2011","journal-title":"J Immunol"},{"key":"B45","doi-asserted-by":"publisher","first-page":"6","DOI":"10.3389\/fimmu.2016.00006","article-title":"TCR signal strength alters T-DC activation and interaction times and directs the outcome of differentiation","volume":"7","author":"van Panhuys","year":"2016","journal-title":"Front Immunol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/S0952-7915(00)00208-9","article-title":"Signal strength in thymic selection and lineage commitment","volume":"13","author":"Hogquist","year":"2001","journal-title":"Curr Opin Immunol"},{"key":"B47","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1182\/blood-2009-01-200378","article-title":"Nonpermissive HLA-DPB1 disparity is a significant independent risk factor for mortality after unrelated hematopoietic stem cell transplantation","volume":"114","author":"Crocchiolo","year":"2009","journal-title":"Blood"},{"key":"B48","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1056\/NEJMoa1500140","article-title":"High HLA-DP expression and graft-versus-host disease","volume":"373","author":"Petersdorf","year":"2015","journal-title":"N Engl J Med"},{"key":"B49","doi-asserted-by":"publisher","first-page":"669","DOI":"10.1056\/NEJMe1505539","article-title":"Immunogenetics of HLA-DP \u2013 a new view of permissible mismatches","volume":"373","author":"Fleischhauer","year":"2015","journal-title":"N Engl J Med"},{"key":"B50","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1111\/ejh.12283","article-title":"Naive subset develops the most important alloreactive response among human CD4+ T lymphocytes in human leukocyte antigen-identical related setting","volume":"92","author":"Cherel","year":"2014","journal-title":"Eur J Haematol"},{"key":"B51","doi-asserted-by":"publisher","first-page":"1024","DOI":"10.3324\/haematol.2010.037481","article-title":"Alloreactive and leukemia-reactive T cells are preferentially derived from naive precursors in healthy donors: implications for immunotherapy with memory T cells","volume":"96","author":"Distler","year":"2011","journal-title":"Haematologica"},{"key":"B52","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/BF00364474","article-title":"HLA class I specific T lymphocyte clones with dual alloreactive functions","volume":"19","author":"Flomenberg","year":"1984","journal-title":"Immunogenetics"},{"key":"B53","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.4049\/jimmunol.133.4.1705","article-title":"OKT4+ cytotoxic T cells can lyse targets via class I molecules and can be blocked by monoclonal antibody against T4 molecules","volume":"133","author":"Strassman","year":"1984","journal-title":"J Immunol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2018.00280\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T02:56:59Z","timestamp":1693537019000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fimmu.2018.00280\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,19]]},"references-count":53,"alternative-id":["10.3389\/fimmu.2018.00280"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2018.00280","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,19]]},"article-number":"280"}}