{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:43:16Z","timestamp":1790995396306,"version":"4.1.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T00:00:00Z","timestamp":1549238400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2018.03150","type":"journal-article","created":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T04:15:31Z","timestamp":1549253731000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Dietary Iron Deficiency Impaired Peripheral Immunity but Did Not Alter Brain Microglia in PRRSV-Infected Neonatal Piglets"],"prefix":"10.3389","volume":"9","author":[{"given":"Brian J.","family":"Leyshon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan P.","family":"Caputo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie M.","family":"Matt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodney W.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,2,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2224","DOI":"10.1016\/S0140-6736(12)61766-8","article-title":"A comparative risk assessment of burden of disease and injury attributable to 67 risk factors and risk factor clusters in 21 regions, 1990\u20132010: a systematic analysis for the Global Burden of Disease Study 2010","volume":"380","author":"Lim","year":"2012","journal-title":"Lancet"},{"key":"B2","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1513\/AnnalsATS.201311-405PS","article-title":"The global burden of respiratory disease","volume":"11","author":"Ferkol","year":"2014","journal-title":"Ann Am Thorac Soc."},{"key":"B3","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/j.biopha.2004.11.009","article-title":"Phagocytic capacity and apoptosis of peripheral blood cells from patients with iron deficiency anemia","volume":"59","author":"Bergman","year":"2005","journal-title":"Biomed Pharmacother."},{"key":"B4","doi-asserted-by":"publisher","first-page":"280","DOI":"10.1016\/j.imbio.2014.09.010","article-title":"'Ride on the ferrous wheel'\u2013the cycle of iron in macrophages in health and disease","volume":"220","author":"Nairz","year":"2015","journal-title":"Immunobiology"},{"key":"B5","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/j.clim.2004.08.011","article-title":"In vitro cytokine production in patients with iron deficiency anemia","volume":"113","author":"Bergman","year":"2004","journal-title":"Clin Immunol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1053\/ajot.2001.28075","article-title":"The association between iron-deficiency anemia and recurrent acute otitis media","volume":"22","author":"Golz","year":"2001","journal-title":"Am J Otolaryngol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1046\/j.1365-2249.2001.01707.x","article-title":"The effects of iron deficiency on lymphocyte cytokine production and activation: preservation of hepatic iron but not at all cost","volume":"126","author":"Jason","year":"2001","journal-title":"Clin Exp Immunol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1080\/10495390500265034","article-title":"Swine in biomedical research: creating the building blocks of animal models","volume":"16","author":"Schook","year":"2005","journal-title":"Anim. Biotechnol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.dci.2014.03.022","article-title":"The porcine innate immune system: an update","volume":"45","author":"Mair","year":"2014","journal-title":"Dev Comparat Immunol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1038\/nrn2297","article-title":"From inflammation to sickness and depression: when the immune system subjugates the brain","volume":"9","author":"Dantzer","year":"2008","journal-title":"Nat Rev Neurosci."},{"key":"B11","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1172\/JCI90603","article-title":"Understanding the functions and relationships of the glymphatic system and meningeal lymphatics","volume":"127","author":"Louveau","year":"2017","journal-title":"J Clin Invest."},{"key":"B12","doi-asserted-by":"publisher","first-page":"4216","DOI":"10.1523\/JNEUROSCI.3441-12.2013","article-title":"Microglia regulate the number of neural precursor cells in the developing cerebral cortex","volume":"33","author":"Cunningham","year":"2013","journal-title":"J Neurosci."},{"key":"B13","doi-asserted-by":"publisher","first-page":"6","DOI":"10.3389\/fncel.2013.00006","article-title":"Janus-faced microglia: beneficial and detrimental consequences of microglial phagocytosis","volume":"7","author":"Sierra","year":"2013","journal-title":"Front Cell Neurosci."},{"key":"B14","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1177\/1073858414530512","article-title":"Microglial dynamics and role in the healthy and diseased brain: a paradigm of functional plasticity","volume":"21","author":"Gomez-Nicola","year":"2015","journal-title":"Neuroscientist"},{"key":"B15","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.bbi.2013.09.009","article-title":"Do prenatal immune activation and maternal iron deficiency interact to affect neurodevelopment and early behavior in rat offspring?","volume":"35","author":"Harvey","year":"2014","journal-title":"Brain Behav Immun"},{"key":"B16","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.bbi.2014.06.005","article-title":"Additive effects of maternal iron deficiency and prenatal immune activation on adult behaviors in rat offspring","volume":"40","author":"Harvey","year":"2014","journal-title":"Brain Behav Immun."},{"key":"B17","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.jpeds.2016.05.013","article-title":"The role of nutrition in brain development: the golden opportunity of the \u201cFirst 1000 Days","volume":"175","author":"Cusick","year":"2016","journal-title":"J Pediatr."},{"key":"B18","doi-asserted-by":"publisher","first-page":"S34","DOI":"10.1301\/nr.2006.may.S34-S43","article-title":"Long-lasting neural and behavioral effects of iron deficiency in infancy","volume":"64","author":"Lozoff","year":"2006","journal-title":"Nutr Rev."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2534","DOI":"10.1093\/jn\/138.12.2534","article-title":"Why iron deficiency is important in infant development","volume":"138","author":"Beard","year":"2008","journal-title":"J Nutr."},{"key":"B20","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.bbi.2014.08.009","article-title":"Early postnatal respiratory viral infection alters hippocampal neurogenesis, cell fate, and neuron morphology in the neonatal piglet","volume":"44","author":"Conrad","year":"2015","journal-title":"Brain Behav Immun."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1420","DOI":"10.3945\/jn.115.223636","article-title":"Postnatal iron deficiency alters brain development in piglets","volume":"146","author":"Leyshon","year":"2016","journal-title":"J Nutr."},{"key":"B22","volume-title":"Nutrient Requirements of Swine: 10th Revised Edition","author":"Council","year":"1998"},{"key":"B23","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.bbi.2016.02.010","article-title":"Peripheral viral infection induced microglial sensome genes and enhanced microglial cell activity in the hippocampus of neonatal piglets","volume":"54","author":"Ji","year":"2016","journal-title":"Brain Behav Immun."},{"key":"B24","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/S0378-1135(96)01347-8","article-title":"Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV)","volume":"56","author":"Duan","year":"1997","journal-title":"Vet Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.vetmic.2014.01.031","article-title":"Up-regulation of pro-inflammatory factors by HP-PRRSV infection in microglia: implications for HP-PRRSV neuropathogenesis","volume":"170","author":"Chen","year":"2014","journal-title":"Vet Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"2120","DOI":"10.1523\/JNEUROSCI.2180-13.2014","article-title":"Respiratory viral infection in neonatal piglets causes marked microglia activation in the hippocampus and deficits in spatial learning","volume":"34","author":"Elmore","year":"2014","journal-title":"J Neurosci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"B28","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1136\/vr.151.12.344","article-title":"Effects of different us isolates of porcine reproductive and respiratory syndrome virus (PRRSV) on blood and bone marrow parameters of experimentally infected pigs","volume":"151","author":"Halbur","year":"2002","journal-title":"Vet Rec."},{"key":"B29","doi-asserted-by":"publisher","first-page":"112","DOI":"10.3945\/an.110.000190","article-title":"The role of iron in learning and memory","volume":"2","author":"Fretham","year":"2011","journal-title":"Adv Nutr."},{"key":"B30","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1159\/000339311","article-title":"Brain growth of the domestic pig (Sus scrofa) from 2 to 24 weeks of age: a longitudinal MRI study","volume":"34","author":"Conrad","year":"2012","journal-title":"Dev Neurosci."},{"key":"B31","doi-asserted-by":"publisher","first-page":"2176","DOI":"10.3945\/jn.115.214650","article-title":"Maternal fish oil supplementation affects the social behavior, brain fatty acid profile, and sickness response of piglets","volume":"145","author":"Clouard","year":"2015","journal-title":"J Nutr."},{"key":"B32","doi-asserted-by":"publisher","first-page":"152","DOI":"10.3389\/fphar.2014.00152","article-title":"Iron at the interface of immunity and infection","volume":"5","author":"Nairz","year":"2014","journal-title":"Front Pharmacol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/j.vetimm.2014.03.008","article-title":"Cytokines transcript levels in lung and lymphoid organs during genotype 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection","volume":"160","author":"Garcia-Nicolas","year":"2014","journal-title":"Vet Immunol Immunopathol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1084\/jem.180.3.969","article-title":"Iron regulates nitric oxide synthase activity by controlling nuclear transcription","volume":"180","author":"Weiss","year":"1994","journal-title":"J Exp Med."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1166","DOI":"10.1016\/j.dci.2011.04.006","article-title":"Porcine regulatory T cells: Mechanisms and T-cell targets of suppression","volume":"35","author":"K\u00e4ser","year":"2011","journal-title":"Dev Compar Immunol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.bbi.2006.09.006","article-title":"Twenty years of research on cytokine-induced sickness behavior","volume":"21","author":"Dantzer","year":"2007","journal-title":"Brain Behav Immun."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1588S","DOI":"10.3945\/ajcn.117.155846","article-title":"Iron assessment to protect the developing brain","volume":"106","author":"Georgieff","year":"2017","journal-title":"Am J Clin Nutr."},{"key":"B38","doi-asserted-by":"publisher","first-page":"2050","DOI":"10.3945\/jn.112.165522","article-title":"Early life iron deficiency impairs spatial cognition in neonatal piglets","volume":"142","author":"Rytych","year":"2012","journal-title":"J Nutr."}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2018.03150\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,2,4]],"date-time":"2019-02-04T04:15:32Z","timestamp":1549253732000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2018.03150\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,4]]},"references-count":38,"alternative-id":["10.3389\/fimmu.2018.03150"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2018.03150","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,4]]},"article-number":"3150"}}