{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:05:49Z","timestamp":1790229949014,"version":"4.1.0"},"reference-count":56,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T00:00:00Z","timestamp":1551830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2019.00418","type":"journal-article","created":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T05:03:54Z","timestamp":1551848634000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":34,"title":["GRA24-Based DNA Vaccine Prolongs Survival in Mice Challenged With a Virulent Toxoplasma gondii Strain"],"prefix":"10.3389","volume":"10","author":[{"given":"Bin","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Lou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianzu","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunhui","family":"Zhuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingming","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,3,6]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1079\/AHR2005100","article-title":"Biology and epidemiology of Toxoplasma gondii in man and animals","volume":"6","author":"Hill","year":"2005","journal-title":"Anim Health Res Rev"},{"key":"B2","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1111\/j.1550-7408.2008.00345.x","article-title":"The history of Toxoplasma gondii\u2013the first 100 years","volume":"55","author":"Dubey","year":"2008","journal-title":"J Eukaryot Microbiol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1016\/j.ijpara.2009.01.005","article-title":"History of the discovery of the life cycle of Toxoplasma gondii","volume":"39","author":"Dubey","year":"2009","journal-title":"Int J Parasitol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis","volume":"39","author":"Pappas","year":"2009","journal-title":"Int J Parasitol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1007\/s00436-012-3120-6","article-title":"Detection of toxoplasmosis in patients with end-stage renal disease by enzyme-linked immunosorbent assay and polymerase chain reaction methods","volume":"112","author":"Saki","year":"2013","journal-title":"Parasitol Res"},{"key":"B6","doi-asserted-by":"publisher","first-page":"389","DOI":"10.3389\/fmicb.2017.00389","article-title":"Toxoplasma gondii infection in immunocompromised patients: a systematic review and meta-analysis","volume":"8","author":"Wang","year":"2017","journal-title":"Front Microbiol"},{"key":"B7","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","author":"Robert-Gangneux","year":"2012","journal-title":"Clin Microbiol Rev"},{"key":"B8","doi-asserted-by":"publisher","first-page":"e90203","DOI":"10.1371\/journal.pone.0090203","article-title":"Toxoplasmosis\u2013a global threat. Correlation of latent toxoplasmosis with specific disease burden in a set of 88 countries","volume":"9","author":"Flegr","year":"2014","journal-title":"PLoS ONE"},{"key":"B9","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.ijpara.2012.09.012","article-title":"Toxoplasma gondii prevalence in farm animals in the United States","volume":"43","author":"Hill","year":"2013","journal-title":"Int J Parasitol"},{"key":"B10","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1111\/acps.12423","article-title":"Beyond the association. Toxoplasma gondii in schizophrenia, bipolar disorder, and addiction: systematic review and meta-analysis","volume":"132","author":"Sutterland","year":"2015","journal-title":"Acta Psychiatr Scand"},{"key":"B11","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/j.meegid.2016.06.053","article-title":"Host and Toxoplasma gondii genetic and non-genetic factors influencing the development of ocular toxoplasmosis: a systematic review","volume":"44","author":"Fernandez","year":"2016","journal-title":"Infect Genet Evol"},{"key":"B12","doi-asserted-by":"publisher","first-page":"e18","DOI":"10.4088\/JCP.15m10133","article-title":"Effects of cumulative herpesviridae and Toxoplasma gondii infections on cognitive function in healthy, bipolar, and schizophrenia subjects","volume":"78","author":"Hamdani","year":"2017","journal-title":"J Clin Psychiatry"},{"key":"B13","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1186\/s12888-018-1821-9","article-title":"Immunoglobulin sub-class distribution in bipolar disorder and schizophrenia: potential relationship with latent Toxoplasma Gondii infection","volume":"18","author":"Hamdani","year":"2018","journal-title":"BMC Psychiatry"},{"key":"B14","doi-asserted-by":"publisher","first-page":"e00057","DOI":"10.1128\/CMR.00057-17","article-title":"Treatment of toxoplasmosis: historical perspective, animal models, and current clinical practice","volume":"31","author":"Dunay","year":"2018","journal-title":"Clin Microbiol Rev"},{"key":"B15","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1136\/vr.129.5.89","article-title":"Vaccination of sheep with a live incomplete strain (S48) of Toxoplasma gondii and their immunity to challenge when pregnant","volume":"129","author":"Buxton","year":"1991","journal-title":"Vet Rec"},{"key":"B16","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/0169-4758(93)90236-9","article-title":"Toxoplasmosis: the first commercial vaccine","volume":"9","author":"Buxton","year":"1993","journal-title":"Parasitol Today"},{"key":"B17","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1586\/14760584.8.2.215","article-title":"Vaccination concepts against Toxoplasma gondii","volume":"8","author":"Garcia","year":"2009","journal-title":"Expert Rev Vaccines"},{"key":"B18","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1586\/14760584.2013.844652","article-title":"Vaccines against Toxoplasma gondii: new developments and perspectives","volume":"12","author":"Zhang","year":"2013","journal-title":"Expert Rev Vaccines"},{"key":"B19","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1586\/erv.09.27","article-title":"Current status of toxoplasmosis vaccine development","volume":"8","author":"Kur","year":"2009","journal-title":"Expert Rev Vaccines"},{"key":"B20","doi-asserted-by":"publisher","first-page":"2148","DOI":"10.1128\/IAI.02756-14","article-title":"Nonreplicating, cyst-defective type II Toxoplasma gondii vaccine strains stimulate protective immunity against acute and chronic infection","volume":"83","author":"Fox","year":"2015","journal-title":"Infect Immun"},{"key":"B21","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1586\/14760584.2015.1098539","article-title":"Recent advances in developing vaccines against Toxoplasma gondii: an update","volume":"14","author":"Zhang","year":"2015","journal-title":"Expert Rev Vaccines"},{"key":"B22","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/s40813-016-0047-9","article-title":"Vaccination against parasites - status quo and the way forward","volume":"2","author":"Joachim","year":"2016","journal-title":"Porcine Health Manag"},{"key":"B23","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1080\/14712598.2018.1413086","article-title":"Moving towards improved vaccines for Toxoplasma gondii","volume":"18","author":"Li","year":"2018","journal-title":"Expert Opin Biol Ther"},{"key":"B24","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1016\/j.pt.2015.02.002","article-title":"Toxoplasma secretory granules: one population or more?","volume":"31","author":"Mercier","year":"2015","journal-title":"Trends Parasitol"},{"key":"B25","doi-asserted-by":"publisher","first-page":"2071","DOI":"10.1084\/jem.20130103","article-title":"A Toxoplasma dense granule protein, GRA24, modulates the early immune response to infection by promoting a direct and sustained host p38 MAPK activation","volume":"210","author":"Braun","year":"2013","journal-title":"J Exp Med"},{"key":"B26","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1111\/cmi.12255","article-title":"Toxoplasma exports dense granule proteins beyond the vacuole to the host cell nucleus and rewires the host genome expression","volume":"16","author":"Bougdour","year":"2014","journal-title":"Cell Microbiol"},{"key":"B27","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.str.2016.10.011","article-title":"Structural basis for the subversion of MAP kinase signaling by an intrinsically disordered parasite secreted agonist","volume":"25","author":"Pellegrini","year":"2017","journal-title":"Structure"},{"key":"B28","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1128\/CMR.00005-17","article-title":"Toxoplasma effectors targeting host signaling and transcription","volume":"30","author":"Hakimi","year":"2017","journal-title":"Clin Microbiol Rev"},{"key":"B29","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1016\/j.vaccine.2011.06.110","article-title":"Protective effect against toxoplasmosis in mice induced by DNA immunization with gene encoding Toxoplasma gondii ROP18","volume":"29","author":"Yuan","year":"2011","journal-title":"Vaccine"},{"key":"B30","doi-asserted-by":"publisher","first-page":"4578","DOI":"10.1016\/j.vaccine.2013.07.058","article-title":"The virulence-related rhoptry protein 5 (ROP5) of Toxoplasma Gondii is a novel vaccine candidate against toxoplasmosis in mice","volume":"31","author":"Zheng","year":"2013","journal-title":"Vaccine"},{"key":"B31","doi-asserted-by":"publisher","first-page":"216","DOI":"10.3389\/fmicb.2017.00216","article-title":"Immuno-efficacy of a T. gondii secreted protein with an altered thrombospondin repeat (TgSPATR) as a novel DNA vaccine candidate against acute toxoplasmosis in BALB\/c Mice","volume":"8","author":"Zheng","year":"2017","journal-title":"Front Microbiol"},{"key":"B32","volume-title":"Points to Consider on Plasmid DNA Vaccines for Preventive Infectious Disease Indications","author":"Research","year":"1996"},{"key":"B33","doi-asserted-by":"publisher","first-page":"2680160","DOI":"10.1155\/2017\/2680160","article-title":"Fundamentals and methods for T- and B-cell epitope prediction","volume":"2017","author":"Sanchez-Trincado","year":"2017","journal-title":"J Immunol Res"},{"key":"B34","doi-asserted-by":"publisher","first-page":"1733","DOI":"10.3389\/fmicb.2017.01733","article-title":"SAG4 DNA and peptide vaccination provides partial protection against T. gondii infection in BALB\/c mice","volume":"8","author":"Zhou","year":"2017","journal-title":"Front Microbiol"},{"key":"B35","doi-asserted-by":"publisher","first-page":"909","DOI":"10.3389\/fmicb.2018.00909","article-title":"Immune protection of rhoptry protein 21 (ROP21) of Toxoplasma gondii as a DNA vaccine against toxoplasmosis","volume":"9","author":"Zhang","year":"2018","journal-title":"Front Microbiol"},{"key":"B36","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.3389\/fmicb.2018.02965","article-title":"Protective efficacy against acute and chronic Toxoplasma gondii infection induced by immunization with the DNA vaccine TgDOC2C","volume":"9","author":"Zhang","year":"2018","journal-title":"Front Microbiol"},{"key":"B37","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.pt.2011.03.009","article-title":"Innate responses to Toxoplasma gondii in mice and humans","volume":"27","author":"Pifer","year":"2011","journal-title":"Trends Parasitol"},{"key":"B38","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1038\/nrmicro2858","article-title":"Modulation of innate immunity by Toxoplasma gondii virulence effectors","volume":"10","author":"Hunter","year":"2012","journal-title":"Nat Rev Microbiol"},{"key":"B39","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1038\/nri3598","article-title":"Innate immunity to Toxoplasma gondii infection","volume":"14","author":"Yarovinsky","year":"2014","journal-title":"Nat Rev Immunol"},{"key":"B40","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1385\/IR:27:2-3:521","article-title":"Induction and regulation of IL-12-dependent host resistance to Toxoplasma gondii","volume":"27","author":"Sher","year":"2003","journal-title":"Immunol Res"},{"key":"B41","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1146\/annurev.immunol.15.1.749","article-title":"Cellular responses to interferon-gamma","volume":"15","author":"Boehm","year":"1997","journal-title":"Annu Rev Immunol"},{"key":"B42","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1111\/j.1365-2249.2008.03692.x","article-title":"Immunity and toxoplasma retinochoroiditis","volume":"153","author":"Wallace","year":"2008","journal-title":"Clin Exp Immunol"},{"key":"B43","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.ijpara.2008.08.002","article-title":"The immunobiology of the innate response to Toxoplasma gondii","volume":"39","author":"Miller","year":"2009","journal-title":"Int J Parasitol"},{"key":"B44","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.mib.2017.10.021","article-title":"Exposing Toxoplasma gondii hiding inside the vacuole: a role for GBPs, autophagy and host cell death","volume":"40","author":"Saeij","year":"2017","journal-title":"Curr Opin Microbiol"},{"key":"B45","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1007\/s00281-012-0339-3","article-title":"Immune response and immunopathology during toxoplasmosis","volume":"34","author":"Dupont","year":"2012","journal-title":"Semin Immunopathol"},{"key":"B46","doi-asserted-by":"publisher","first-page":"e31","DOI":"10.1017\/S1462399411002018","article-title":"Interferon-gamma- and perforin-mediated immune responses for resistance against Toxoplasma gondii in the brain","volume":"13","author":"Suzuki","year":"2011","journal-title":"Expert Rev Mol Med"},{"key":"B47","doi-asserted-by":"publisher","first-page":"730","DOI":"10.3389\/fimmu.2017.00730","article-title":"Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice","volume":"8","author":"Wang","year":"2017","journal-title":"Front Immunol"},{"key":"B48","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1038\/sj.cr.7310018","article-title":"IRF family proteins and type I interferon induction in dendritic cells","volume":"16","author":"Tailor","year":"2006","journal-title":"Cell Res"},{"key":"B49","doi-asserted-by":"publisher","first-page":"4818","DOI":"10.4049\/jimmunol.1301301","article-title":"Cooperation of TLR12 and TLR11 in the IRF8-dependent IL-12 response to Toxoplasma gondii profilin","volume":"191","author":"Raetz","year":"2013","journal-title":"J Immunol"},{"key":"B50","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1016\/j.immuni.2012.09.007","article-title":"The transcription factor T-bet is induced by multiple pathways and prevents an endogenous Th2 cell program during Th1 cell responses","volume":"37","author":"Zhu","year":"2012","journal-title":"Immunity"},{"key":"B51","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1051\/parasite\/2017045","article-title":"Immune responses and protection after DNA vaccination against Toxoplasma gondii calcium-dependent protein kinase 2 (TgCDPK2)","volume":"24","author":"Chen","year":"2017","journal-title":"Parasite"},{"key":"B52","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1186\/s12879-017-2507-5","article-title":"Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB\/c mice","volume":"17","author":"Liu","year":"2017","journal-title":"BMC Infect Dis"},{"key":"B53","doi-asserted-by":"publisher","first-page":"1295038","DOI":"10.1155\/2017\/1295038","article-title":"Resistance to Chronic Toxoplasma gondii infection induced by a DNA vaccine expressing GRA16","volume":"2017","author":"Hu","year":"2017","journal-title":"Biomed Res Int"},{"key":"B54","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1586\/14760584.2015.1011133","article-title":"DNA vaccination with genes encoding Toxoplasma gondii antigens ROP5 and GRA15 induces protective immunity against toxoplasmosis in Kunming mice","volume":"14","author":"Chen","year":"2015","journal-title":"Expert Rev Vaccines"},{"key":"B55","doi-asserted-by":"publisher","first-page":"596","DOI":"10.1016\/j.parint.2017.06.002","article-title":"SAG5B and SAG5C combined vaccine protects mice against Toxoplasma gondii infection","volume":"66","author":"Lu","year":"2017","journal-title":"Parasitol Int"},{"key":"B56","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.exppara.2018.04.003","article-title":"Immuno-efficacy of DNA vaccines encoding PLP1 and ROP18 against experimental Toxoplasma gondii infection in mice","volume":"188","author":"Chen","year":"2018","journal-title":"Exp Parasitol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2019.00418\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T05:03:56Z","timestamp":1551848636000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2019.00418\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,6]]},"references-count":56,"alternative-id":["10.3389\/fimmu.2019.00418"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2019.00418","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,6]]},"article-number":"418"}}