{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:17:37Z","timestamp":1787066257706,"version":"build-2736575974"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T00:00:00Z","timestamp":1555372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["149966"],"award-info":[{"award-number":["149966"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["169674"],"award-info":[{"award-number":["169674"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008486","name":"Schweizerische Multiple Sklerose Gesellschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008486","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008038","name":"Neuromuscular Research Association Basel","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2019.00829","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T01:10:07Z","timestamp":1555377007000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B Cells"],"prefix":"10.3389","volume":"10","author":[{"given":"Maria","family":"Zimmermann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Rose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyein","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Rita","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Callegari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammedyaseen","family":"Syedbasha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Kaufmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Egli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raija L. P.","family":"Lindberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludwig","family":"Kappos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Traggiai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas S. R.","family":"Sanderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Derfuss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,4,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.jim.2003.12.015","article-title":"Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system","volume":"286","author":"Crotty","year":"2004","journal-title":"J Immunol Methods."},{"key":"B2","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/j.jim.2008.11.012","article-title":"A method for identification of HIV gp140 binding memory B cells in human blood","volume":"343","author":"Scheid","year":"2009","journal-title":"J Immunol Methods."},{"key":"B3","doi-asserted-by":"publisher","first-page":"2065","DOI":"10.1084\/jem.20112272","article-title":"Deletion and anergy of polyclonal B cells specific for ubiquitous membrane-bound self-antigen","volume":"209","author":"Taylor","year":"2012","journal-title":"J Exp Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1126\/sciimmunol.aan2676","article-title":"Preferential induction of cross-group influenza A hemagglutinin stem-specific memory B cells after H7N9 immunization in humans","volume":"2","author":"Andrews","year":"2017","journal-title":"Sci Immunol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1469","DOI":"10.1084\/jem.20120423","article-title":"Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein","volume":"209","author":"Klein","year":"2012","journal-title":"J Exp Med."},{"key":"B6","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.autrev.2009.03.012","article-title":"TSH receptor autoantibodies","volume":"9","author":"Michalek","year":"2009","journal-title":"Autoimmun Rev."},{"key":"B7","doi-asserted-by":"publisher","first-page":"5866","DOI":"10.1523\/JNEUROSCI.0167-10.2010","article-title":"Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis","volume":"30","author":"Hughes","year":"2010","journal-title":"J Neurosci."},{"key":"B8","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1212\/WNL.0b013e318248dec1","article-title":"Serologic diagnosis of NMO: a multicenter comparison of aquaporin-4-IgG assays","volume":"78","author":"Waters","year":"2012","journal-title":"Neurology."},{"key":"B9","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1073\/pnas.1614472114","article-title":"Cocapture of cognate and bystander antigens can activate autoreactive B cells","volume":"114","author":"Sanderson","year":"2017","journal-title":"Proc Natl Acad Sci USA."},{"key":"B10","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1038\/35078099","article-title":"B cells acquire antigen from target cells after synapse formation","volume":"411","author":"Batista","year":"2001","journal-title":"Nature."},{"key":"B11","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/j.molimm.2018.07.018","article-title":"Mechanics of antigen extraction in the B cell synapse","volume":"101","author":"Spillane","year":"2018","journal-title":"Mol Immunol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1084\/jem.188.1.169","article-title":"B lymphocytes producing demyelinating autoantibodies: development and function in gene-targeted transgenic mice","volume":"188","author":"Litzenburger","year":"1998","journal-title":"J Exp Med."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1940","DOI":"10.1093\/brain\/awn092","article-title":"IgG1 antibodies to acetylcholine receptors in \u2018seronegative' myasthenia gravis","volume":"131","author":"Leite","year":"2008","journal-title":"Brain."},{"key":"B14","author":"Ellis","year":"2017","journal-title":"FlowCore: Basic Structures for Flow Cytometry Data"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3791\/55833","article-title":"An optimized hemagglutination inhibition (HI) assay to quantify influenza-specific antibody titers","author":"Kaufmann","year":"2017","journal-title":"J Vis Exp."},{"key":"B16","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1016\/j.immuni.2019.02.003","article-title":"Human Memory B Cells harbor diverse cross-neutralizing antibodies against BK and JC polyomaviruses","volume":"50","author":"Lindner","year":"2019","journal-title":"Immunity."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1907","DOI":"10.1038\/nprot.2013.117","article-title":"Isolation of human monoclonal antibodies from peripheral blood B cells","volume":"8","author":"Huang","year":"2013","journal-title":"Nat Protoc."},{"key":"B18","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1038\/nature12637","article-title":"Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus","volume":"503","author":"Dougan","year":"2013","journal-title":"Nature"},{"key":"B19","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1084\/jem.20101352","article-title":"Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infection","volume":"208","author":"Wrammert","year":"2011","journal-title":"J Exp Med."},{"key":"B20","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/1743-8977-10-2","article-title":"Deciphering the mechanisms of cellular uptake of engineered nanoparticles by accurate evaluation of internalization using imaging flow cytometry","volume":"10","author":"Vranic","year":"2013","journal-title":"Part Fibre Toxicol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1083\/jcb.201607064","article-title":"B cell antigen extraction is regulated by physical properties of antigen-presenting cells","volume":"216","author":"Spillane","year":"2017","journal-title":"J Cell Biol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"4047","DOI":"10.1128\/JVI.03422-13","article-title":"Flow cytometry reveals that H5N1 vaccination elicits cross-reactive stem-directed antibodies from multiple Ig heavy-chain lineages","volume":"88","author":"Whittle","year":"2014","journal-title":"J Virol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"e1004103","DOI":"10.1371\/journal.ppat.1004103","article-title":"Molecular signatures of hemagglutinin stem-directed heterosubtypic human neutralizing antibodies against influenza A viruses","volume":"10","author":"Avnir","year":"2014","journal-title":"PLoS Pathog."},{"key":"B24","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1038\/nature11371","article-title":"Structural and genetic basis for development of broadly neutralizing influenza antibodies","volume":"489","author":"Lingwood","year":"2012","journal-title":"Nature."},{"key":"B25","doi-asserted-by":"publisher","first-page":"418","DOI":"10.1038\/nature13764","article-title":"Rapid development of broadly influenza neutralizing antibodies through redundant mutations","volume":"516","author":"Pappas","year":"2014","journal-title":"Nature."},{"key":"B26","doi-asserted-by":"publisher","first-page":"2259","DOI":"10.1073\/pnas.1317793111","article-title":"Identification and characterization of the constituent human serum antibodies elicited by vaccination","volume":"111","author":"Lavinder","year":"2014","journal-title":"Proc Natl Acad Sci USA."},{"key":"B27","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.coi.2015.06.009","article-title":"Serology in the 21st century: the molecular-level analysis of the serum antibody repertoire","volume":"35","author":"Wine","year":"2015","journal-title":"Curr Opin Immunol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1038\/nm.3743","article-title":"In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire","volume":"21","author":"DeKosky","year":"2015","journal-title":"Nat Med."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1226","DOI":"10.1038\/ni.3533","article-title":"Defining antigen-specific plasmablast and memory B cell subsets in human blood after viral infection or vaccination","volume":"17","author":"Ellebedy","year":"2016","journal-title":"Nat Immunol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"e0185976","DOI":"10.1371\/journal.pone.0185976","article-title":"Analysis of peripheral B cells and autoantibodies against the anti-nicotinic acetylcholine receptor derived from patients with myasthenia gravis using single-cell manipulation tools","volume":"12","author":"Makino","year":"2017","journal-title":"PLoS ONE"},{"key":"B31","doi-asserted-by":"publisher","first-page":"13898","DOI":"10.1523\/JNEUROSCI.2833-09.2009","article-title":"Main immunogenic region structure promotes binding of conformation-dependent myasthenia gravis autoantibodies, nicotinic acetylcholine receptor conformation maturation, and agonist sensitivity","volume":"29","author":"Luo","year":"2009","journal-title":"J Neurosci."},{"key":"B32","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1038\/ni.1946","article-title":"B cell follicles and antigen encounters of the third kind","volume":"11","author":"Cyster","year":"2010","journal-title":"Nat Immunol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/j.immuni.2011.07.008","article-title":"Polarized secretion of lysosomes at the B cell synapse couples antigen extraction to processing and presentation","volume":"35","author":"Yuseff","year":"2011","journal-title":"Immunity."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1038\/nature09118","article-title":"Subcapsular sinus macrophages prevent CNS invasion on peripheral infection with a neurotropic virus","volume":"465","author":"Iannacone","year":"2010","journal-title":"Nature."},{"key":"B35","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1038\/nature06287","article-title":"Subcapsular sinus macrophages in lymph nodes clear lymph-borne viruses and present them to antiviral B cells","volume":"450","author":"Junt","year":"2007","journal-title":"Nature"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2019.00829\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T01:10:11Z","timestamp":1555377011000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2019.00829\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,16]]},"references-count":35,"alternative-id":["10.3389\/fimmu.2019.00829"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2019.00829","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,16]]},"article-number":"829"}}