{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:53:11Z","timestamp":1790650391932,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T00:00:00Z","timestamp":1556582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["643381"],"award-info":[{"award-number":["643381"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2019.00894","type":"journal-article","created":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T09:49:42Z","timestamp":1556617782000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":36,"title":["RUTI Vaccination Enhances Inhibition of Mycobacterial Growth ex vivo and Induces a Shift of Monocyte Phenotype in Mice"],"prefix":"10.3389","volume":"10","author":[{"given":"Satria A.","family":"Prabowo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Painter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Zelmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven G.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Seifert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merce","family":"Amat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pere-Joan","family":"Cardona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen A.","family":"Fletcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,4,30]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e183","DOI":"10.1016\/s1473-3099(18)30110-5","article-title":"Tuberculosis: progress and advances in development of new drugs, treatment regimens, and host-directed therapies","volume":"18","author":"Tiberi","year":"2018","journal-title":"Lancet Infect Dis."},{"key":"B2","doi-asserted-by":"publisher","first-page":"1536","DOI":"10.3389\/fmicb.2016.01536","article-title":"The progress of therapeutic vaccination with regard to tuberculosis","volume":"7","author":"Cardona","year":"2016","journal-title":"Front Microbiol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1093\/trstmh\/trw016","article-title":"TB vaccine development and the End TB Strategy: importance and current status","volume":"110","author":"Fletcher","year":"2016","journal-title":"Trans R Soc Trop Med Hyg."},{"key":"B4","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.micinf.2009.10.006","article-title":"Tuberculin immunotherapy: its history and lessons to be learned","volume":"12","author":"Vilaplana","year":"2010","journal-title":"Microbes Infect."},{"key":"B5","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.tube.2006.01.024","article-title":"RUTI: a new chance to shorten the treatment of latent tuberculosis infection","volume":"86","author":"Cardona","year":"2006","journal-title":"Tuberculosis."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1229","DOI":"10.1128\/CVI.00094-08","article-title":"Induction of a specific strong polyantigenic cellular immune response after short-term chemotherapy controls bacillary reactivation in murine and guinea pig experimental models of tuberculosis","volume":"15","author":"Guirado","year":"2008","journal-title":"Clin Vaccine Immunol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"e20404","DOI":"10.1371\/journal.pone.0020404","article-title":"Prophylactic effect of a therapeutic vaccine against TB based on fragments of Mycobacterium tuberculosis","volume":"6","author":"Vilaplana","year":"2011","journal-title":"PLoS ONE"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1016\/j.vaccine.2009.09.134","article-title":"Double-blind, randomized, placebo-controlled Phase I Clinical Trial of the therapeutical antituberculous vaccine RUTI","volume":"28","author":"Vilaplana","year":"2010","journal-title":"Vaccine."},{"key":"B9","doi-asserted-by":"publisher","first-page":"e89612","DOI":"10.1371\/journal.pone.0089612","article-title":"Safety, tolerability, and immunogenicity of the novel antituberculous vaccine RUTI: randomized, placebo-controlled phase II clinical trial in patients with latent tuberculosis infection","volume":"9","author":"Nell","year":"2014","journal-title":"PLoS ONE"},{"key":"B10","doi-asserted-by":"publisher","first-page":"ftw016","DOI":"10.1093\/femspd\/ftw016","article-title":"Towards new TB vaccines: what are the challenges?","volume":"74","author":"Dockrell","year":"2016","journal-title":"Pathog Dis."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1101\/020560","article-title":"Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes","author":"Zelmer","year":"2015","journal-title":"bioRxiv"},{"key":"B12","doi-asserted-by":"publisher","first-page":"4656","DOI":"10.1016\/j.vaccine.2016.07.058","article-title":"In vitro mycobacterial growth inhibition assays: A tool for the assessment of protective immunity and evaluation of tuberculosis vaccine efficacy","volume":"34","author":"Tanner","year":"2016","journal-title":"Vaccine."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1128\/CVI.00067-09","article-title":"Development of a murine mycobacterial growth inhibition assay for evaluating vaccines against Mycobacterium tuberculosis","volume":"16","author":"Parra","year":"2009","journal-title":"Clin Vaccine Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"1683","DOI":"10.1128\/CVI.00427-13","article-title":"Inhibition of mycobacterial growth in vitro following primary but not secondary vaccination with Mycobacterium bovis BCG","volume":"20","author":"Fletcher","year":"2013","journal-title":"Clin Vaccine Immunol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1186\/s12879-016-1751-4","article-title":"A new tool for tuberculosis vaccine screening: ex vivo mycobacterial growth inhibition assay indicates BCG-mediated protection in a murine model of tuberculosis","volume":"16","author":"Zelmer","year":"2016","journal-title":"BMC Infect Dis."},{"key":"B16","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/j.tube.2013.04.007","article-title":"Mycobacterial growth inhibition in murine splenocytes as a surrogate for protection against Mycobacterium tuberculosis (M. tb)","volume":"93","author":"Marsay","year":"2013","journal-title":"Tuberculosis."},{"key":"B17","doi-asserted-by":"publisher","first-page":"5298","DOI":"10.1016\/j.vaccine.2016.09.002","article-title":"Polyfunctional CD4 T-cells correlate with in vitro mycobacterial growth inhibition following Mycobacterium bovis BCG-vaccination of infants","volume":"34","author":"Smith","year":"2016","journal-title":"Vaccine."},{"key":"B18","doi-asserted-by":"publisher","first-page":"2830","DOI":"10.1038\/s41598-017-02116-1","article-title":"Optimisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis","volume":"7","author":"Jensen","year":"2017","journal-title":"Sci Rep."},{"key":"B19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/2050-7771-2-1","article-title":"Monocyte and macrophage differentiation: circulation inflammatory monocyte as biomarker for inflammatory diseases","volume":"2","author":"Yang","year":"2014","journal-title":"Biomark Res."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/microbiolspec.MCHD-0033-2016","article-title":"Murine monocytes: origins, subsets, fates, and functions","volume":"4","author":"Mildner","year":"2016","journal-title":"Microbiol Spectr."},{"key":"B21","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/j.immuni.2017.04.018","article-title":"Genomic characterization of murine monocytes reveals C\/EBPbeta transcription factor dependence of Ly6C- cells","volume":"46","author":"Mildner","year":"2017","journal-title":"Immunity."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1726","DOI":"10.3389\/fimmu.2018.01726","article-title":"Monocyte subsets: phenotypes and function in tuberculosis infection","volume":"9","author":"Sampath","year":"2018","journal-title":"Front Immunol."},{"key":"B23","doi-asserted-by":"crossref","first-page":"2243","DOI":"10.1084\/jem.178.6.2243","article-title":"Disseminated tuberculosis in interferon gamma gene-disrupted mice","volume":"178","author":"Cooper","year":"1993","journal-title":"J Exp Med."},{"key":"B24","doi-asserted-by":"publisher","first-page":"454","DOI":"10.1016\/j.smim.2014.09.008","article-title":"Mendelian susceptibility to mycobacterial disease: genetic, immunological, and clinical features of inborn errors of IFN-\u03b3 immunity","volume":"26","author":"Bustamante","year":"2014","journal-title":"Semin Immunol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"11290","DOI":"10.1038\/ncomms11290","article-title":"T-cell activation is an immune correlate of risk in BCG vaccinated infants","volume":"7","author":"Fletcher","year":"2016","journal-title":"Nat Commun."},{"key":"B26","doi-asserted-by":"publisher","first-page":"1073","DOI":"10.1164\/rccm.201003-0334OC","article-title":"Specific T cell frequency and cytokine expression profile do not correlate with protection against tuberculosis after bacillus Calmette-Guerin vaccination of newborns","volume":"182","author":"Kagina","year":"2010","journal-title":"Am J Respir Crit Care Med."},{"key":"B27","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/j.1365-2249.2012.04614.x","article-title":"Is interferon-gamma the right marker for bacille Calmette-Guerin-induced immune protection? The missing link in our understanding of tuberculosis immunology","volume":"169","author":"Abebe","year":"2012","journal-title":"Clin Exp Immunol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1016\/j.ebiom.2015.09.027","article-title":"Distinct transcriptional and anti-mycobacterial profiles of peripheral blood monocytes dependent on the ratio of monocytes: lymphocytes","volume":"2","author":"Naranbhai","year":"2015","journal-title":"EBiomedicine"},{"key":"B29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1128\/CVI.00142-17","article-title":"The cross-species mycobacterial growth inhibition assay (MGIA) project, 2010-2014","volume":"24","author":"Brennan","year":"2017","journal-title":"Clin Vaccine Immunol."},{"key":"B30","unstructured":"TannerR\n          Development of Mycobacterial Growth Inhibition Assays for the Early Evaluation and Gating of Novel TB Vaccine Candidates.2015"},{"key":"B31","unstructured":"AnwarS\n          Impact of Helminth Infection on Antimycobacterial Immune Responses in UK Migrants.2017"},{"key":"B32","doi-asserted-by":"publisher","first-page":"14480","DOI":"10.1038\/s41598-018-32755-x","article-title":"Immunological correlates of mycobacterial growth inhibition describe a spectrum of tuberculosis infection","volume":"8","author":"O'Shea","year":"2018","journal-title":"Sci Rep."},{"key":"B33","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1038\/ni.2063","article-title":"The transcription factor NR4A1 (Nur77) controls bone marrow differentiation and the survival of Ly6C- monocytes","volume":"12","author":"Hanna","year":"2011","journal-title":"Nat Immunol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1016\/j.immuni.2016.10.011","article-title":"Deleting an Nr4a1 super-enhancer subdomain ablates Ly6Clow monocytes while preserving macrophage gene function","volume":"45","author":"Thomas","year":"2016","journal-title":"Immunity."},{"key":"B35","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1126\/science.1142883","article-title":"Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior","volume":"317","author":"Auffray","year":"2007","journal-title":"Science."},{"key":"B36","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1146\/annurev.immunol.26.021607.090326","article-title":"Monocyte-mediated defense against microbial pathogens","volume":"26","author":"Serbina","year":"2008","journal-title":"Annu. Rev. Immunol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.immuni.2012.12.001","article-title":"Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis","volume":"38","author":"Yona","year":"2013","journal-title":"Immunity"},{"key":"B38","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1016\/j.immuni.2017.04.010","article-title":"Understanding spontaneous conversion: the case of the Ly6C- monocyte","volume":"46","author":"Polletti","year":"2017","journal-title":"Immunity."},{"key":"B39","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1146\/annurev-immunol-032712-095939","article-title":"The immune response in tuberculosis","volume":"31","author":"O'Garra","year":"2013","journal-title":"Annu Rev Immunol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1007\/s00430-012-0278-6","article-title":"Targeting multidrug-resistant tuberculosis (MDR-TB) by therapeutic vaccines","volume":"202","author":"Prabowo","year":"2013","journal-title":"Med Microbiol Immunol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1126\/sciimmunol.aar4135","article-title":"Mitochondrial cyclophilin D regulates T cell metabolic responses and disease tolerance to tuberculosis","volume":"3","author":"Tzelepis","year":"2018","journal-title":"Sci Immunol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1016\/j.vaccine.2004.09.008","article-title":"Immunotherapy with fragmented Mycobacterium tuberculosis cells increases the effectiveness of chemotherapy against a chronical infection in a murine model of tuberculosis","volume":"23","author":"Cardona","year":"2005","journal-title":"Vaccine."},{"key":"B43","doi-asserted-by":"publisher","first-page":"3162","DOI":"10.1016\/j.vaccine.2014.03.047","article-title":"Therapeutic vaccines for tuberculosis\u2014A systematic review","volume":"32","author":"Gr\u00f6schel","year":"2014","journal-title":"Vaccine."},{"key":"B44","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1172\/JCI97508","article-title":"Mycobacterial growth inhibition is associated with trained innate immunity","volume":"128","author":"Joosten","year":"2018","journal-title":"J Clin Invest."},{"key":"B45","doi-asserted-by":"publisher","first-page":"17537","DOI":"10.1073\/pnas.1202870109","article-title":"Bacille Calmette-Guerin induces NOD2-dependent nonspecific protection from reinfection via epigenetic reprogramming of monocytes","volume":"109","author":"Kleinnijenhuis","year":"2012","journal-title":"Proc Natl Acad Sci USA."},{"key":"B46","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.smim.2018.06.002","article-title":"Non-specific effects of vaccines: current evidence and potential implications","volume":"39","author":"de Bree","year":"2018","journal-title":"Semin Immunol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.coph.2018.04.007","article-title":"Trained immunity as a novel therapeutic strategy","volume":"41","author":"Mourits","year":"2018","journal-title":"Curr Opin Pharmacol."}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2019.00894\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,30]],"date-time":"2019-04-30T09:49:45Z","timestamp":1556617785000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2019.00894\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,30]]},"references-count":47,"alternative-id":["10.3389\/fimmu.2019.00894"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2019.00894","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,30]]},"article-number":"894"}}