{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:36:03Z","timestamp":1781584563552,"version":"3.54.5"},"reference-count":135,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007601","name":"Horizon 2020","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007601","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2020.00190","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T09:49:07Z","timestamp":1582278547000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":28,"title":["Current Challenges in the Identification of Pre-Erythrocytic Malaria Vaccine Candidate Antigens"],"prefix":"10.3389","volume":"11","author":[{"given":"Paulo","family":"Bettencourt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,2,21]]},"reference":[{"key":"B1","volume-title":"World Malaria Report 2018","year":"2018"},{"key":"B2","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/1756-3305-7-76","article-title":"An online tool for mapping insecticide resistance in major Anopheles vectors of human malaria parasites and review of resistance status for the Afrotropical region","volume":"7","author":"Knox","year":"2014","journal-title":"Parasit Vectors."},{"key":"B3","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0140-6736(15)60721-8","article-title":"Efficacy and safety of RTS,S\/AS01 malaria vaccine with or without a booster dose in infants and children in Africa: final results of a phase 3, individually randomised, controlled trial","volume":"386","year":"2015","journal-title":"Lancet."},{"key":"B4","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1038\/s41541-017-0035-3","article-title":"Advances in malaria vaccine development: report from the 2017 malaria vaccine symposium","volume":"2","author":"Coelho","year":"2017","journal-title":"NPJ Vaccines."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/bs.apar.2015.03.001","article-title":"Recent developments in malaria vaccinology","volume":"88","author":"Halbroth","year":"2015","journal-title":"Adv Parasitol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1700","DOI":"10.1016\/S0140-6736(13)62238-2","article-title":"Malaria vaccine technology roadmap","volume":"382","author":"Moorthy","year":"2013","journal-title":"Lancet."},{"key":"B7","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1056\/NEJM199701093360202","article-title":"A preliminary evaluation of a recombinant circumsporozoite protein vaccine against Plasmodium falciparum malaria. RTS,S Malaria Vaccine Evaluation Group","volume":"336","author":"Stoute","year":"1997","journal-title":"N Engl J Med."},{"key":"B8","doi-asserted-by":"publisher","first-page":"e20775","DOI":"10.1371\/journal.pone.0020775","article-title":"Protective immunity induced with the RTS,S\/AS vaccine is associated with IL-2 and TNF-alpha producing effector and central memory CD4 T cells","volume":"6","author":"Lumsden","year":"2011","journal-title":"PLoS ONE."},{"key":"B9","doi-asserted-by":"publisher","first-page":"6961","DOI":"10.4049\/jimmunol.171.12.6961","article-title":"Protective immunity induced with malaria vaccine, RTS,S, is linked to Plasmodium falciparum circumsporozoite protein-specific CD4+ and CD8+ T cells producing IFN-gamma","volume":"171","author":"Sun","year":"2003","journal-title":"J Immunol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1038\/216160a0","article-title":"Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei","volume":"216","author":"Nussenzweig","year":"1967","journal-title":"Nature."},{"key":"B11","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.2307\/3276987","article-title":"Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. II Effects of radiation on sporozoites","volume":"54","author":"Vanderberg","year":"1968","journal-title":"J Parasitol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1073\/pnas.85.2.573","article-title":"CD8+ T cells (cytotoxic\/suppressors) are required for protection in mice immunized with malaria sporozoites","volume":"85","author":"Weiss","year":"1988","journal-title":"Proc Natl Acad Sci USA."},{"key":"B13","doi-asserted-by":"crossref","first-page":"4345","DOI":"10.1002\/(SICI)1521-4141(199812)28:12<4345::AID-IMMU4345>3.0.CO;2-P","article-title":"Protection from Plasmodium berghei infection by priming and boosting T cells to a single class I-restricted epitope with recombinant carriers suitable for human use","volume":"28","author":"Plebanski","year":"1998","journal-title":"Eur J Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1038\/341323a0","article-title":"Cloned cytotoxic T cells recognize an epitope in the circumsporozoite protein and protect against malaria","volume":"341","author":"Romero","year":"1989","journal-title":"Nature."},{"key":"B15","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/S1074-7613(00)80513-0","article-title":"Degenerate cytotoxic T cell epitopes from P. falciparum restricted by multiple HLA-A and HLA-B supertype alleles","volume":"7","author":"Doolan","year":"1997","journal-title":"Immunity."},{"key":"B16","doi-asserted-by":"publisher","first-page":"9952","DOI":"10.1073\/pnas.1633254100","article-title":"Identification of Plasmodium falciparum antigens by antigenic analysis of genomic and proteomic data","volume":"100","author":"Doolan","year":"2003","journal-title":"Proc Natl Acad Sci USA."},{"key":"B17","doi-asserted-by":"publisher","first-page":"2836","DOI":"10.1038\/ncomms3836","article-title":"Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation","volume":"4","author":"Ewer","year":"","journal-title":"Nat Commun."},{"key":"B18","doi-asserted-by":"publisher","first-page":"e2012013","DOI":"10.4084\/mjhid.2012.013","article-title":"Biology of human malaria plasmodia including Plasmodium knowlesi","volume":"4","author":"Antinori","year":"2012","journal-title":"Mediterr J Hematol Infect Dis."},{"key":"B19","doi-asserted-by":"publisher","first-page":"4363","DOI":"10.1128\/IAI.73.7.4363-4369.2005","article-title":"Quantitative dynamics of Plasmodium yoelii sporozoite transmission by infected anopheline mosquitoes","volume":"73","author":"Medica","year":"2005","journal-title":"Infect Immun."},{"key":"B20","doi-asserted-by":"publisher","first-page":"e07789","DOI":"10.7554\/eLife.07789","article-title":"Longitudinal analysis of Plasmodium sporozoite motility in the dermis reveals component of blood vessel recognition","volume":"4","author":"Hopp","year":"2015","journal-title":"eLife"},{"key":"B21","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1136\/bmj.1.4550.547","article-title":"The pre-erythrocytic stage of human malaria, Plasmodium vivax","volume":"1","author":"Shortt","year":"1948","journal-title":"Br Med J."},{"key":"B22","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1038\/nm785","article-title":"Migration through host cells activates Plasmodium sporozoites for infection","volume":"8","author":"Mota","year":"2002","journal-title":"Nat Med."},{"key":"B23","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1126\/science.291.5501.141","article-title":"Migration of Plasmodium sporozoites through cells before infection","volume":"291","author":"Mota","year":"2001","journal-title":"Science."},{"key":"B24","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.ijmm.2012.07.011","article-title":"Molecular make-up of the Plasmodium parasitophorous vacuolar membrane","volume":"302","author":"Spielmann","year":"2012","journal-title":"Int J Med Microbiol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"2806","DOI":"10.1098\/rstb.2011.0091","article-title":"Vaccines against malaria","volume":"366","author":"Hill","year":"2011","journal-title":"Philos Trans R Soc Lond B Biol Sci."},{"key":"B26","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1038\/nature01097","article-title":"Genome sequence of the human malaria parasite Plasmodium falciparum","volume":"419","author":"Gardner","year":"2002","journal-title":"Nature."},{"key":"B27","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1038\/nature01107","article-title":"A proteomic view of the Plasmodium falciparum life cycle","volume":"419","author":"Florens","year":"2002","journal-title":"Nature."},{"key":"B28","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1126\/science.6985745","article-title":"Hybridoma produces protective antibodies directed against the sporozoite stage of malaria parasite","volume":"207","author":"Yoshida","year":"1980","journal-title":"Science."},{"key":"B29","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1038\/329164a0","article-title":"A liver-stage-specific antigen of Plasmodium falciparum characterized by gene cloning","volume":"329","author":"Guerin-Marchand","year":"1987","journal-title":"Nature."},{"key":"B30","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1038\/335079a0","article-title":"A highly conserved amino-acid sequence in thrombospondin, properdin and in proteins from sporozoites and blood stages of a human malaria parasite","volume":"335","author":"Robson","year":"1988","journal-title":"Nature."},{"key":"B31","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/0166-6851(94)00012-3","article-title":"Cloning and characterization of a novel Plasmodium falciparum sporozoite surface antigen, STARP","volume":"64","author":"Fidock","year":"1994","journal-title":"Mol Biochem Parasitol."},{"key":"B32","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.4049\/jimmunol.156.8.2874","article-title":"A novel Plasmodium falciparum sporozoite and liver stage antigen (SALSA) defines major B, T helper, and CTL epitopes","volume":"156","author":"Bottius","year":"1996","journal-title":"J Immunol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1038\/81366","article-title":"Protection against Plasmodium falciparum malaria in chimpanzees by immunization with the conserved pre-erythrocytic liver-stage antigen 3","volume":"6","author":"Daubersies","year":"2000","journal-title":"Nat Med."},{"key":"B34","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1038\/nature01111","article-title":"Analysis of the Plasmodium falciparum proteome by high-accuracy mass spectrometry","volume":"419","author":"Lasonder","year":"2002","journal-title":"Nature."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1221","DOI":"10.1046\/j.1365-2958.2003.03909.x","article-title":"Differential transcriptome profiling identifies Plasmodium genes encoding pre-erythrocytic stage-specific proteins","volume":"51","author":"Kaiser","year":"2004","journal-title":"Mol Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.molbiopara.2004.06.001","article-title":"Transcriptome of axenic liver stages of Plasmodium yoelii","volume":"137","author":"Wang","year":"2004","journal-title":"Mol Biochem Parasitol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.molbiopara.2005.03.018","article-title":"Transcriptional analysis of in vivo Plasmodium yoelii liver stage gene expression","volume":"142","author":"Sacci","year":"2005","journal-title":"Mol. Biochem. Parasitol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1073\/pnas.0710780104","article-title":"A combined transcriptome and proteome survey of malaria parasite liver stages","volume":"105","author":"Tarun","year":"2008","journal-title":"Proc Natl Acad Sci U.S.A."},{"key":"B39","doi-asserted-by":"publisher","first-page":"e1000121","DOI":"10.1371\/journal.ppat.1000121","article-title":"Temperature shift and host cell contact up-regulate sporozoite expression of Plasmodium falciparum genes involved in hepatocyte infection","volume":"4","author":"Siau","year":"2008","journal-title":"PLoS Pathog."},{"key":"B40","doi-asserted-by":"publisher","first-page":"e10267","DOI":"10.1371\/journal.pone.0010267","article-title":"Transcriptional analysis of the pre-erythrocytic stages of the rodent malaria parasite, Plasmodium yoelii","volume":"5","author":"Williams","year":"2010","journal-title":"PLoS ONE."},{"key":"B41","doi-asserted-by":"publisher","first-page":"M111","DOI":"10.1074\/mcp.M111.007948","article-title":"Sterile protective immunity to malaria is associated with a panel of novel P. falciparum antigens","volume":"10","author":"Trieu","year":"2011","journal-title":"Mol. Cell. Proteomics."},{"key":"B42","doi-asserted-by":"publisher","first-page":"e0136109","DOI":"10.1371\/journal.pone.0136109","article-title":"Discovery of novel Plasmodium falciparum pre-erythrocytic antigens for vaccine development","volume":"10","author":"Aguiar","year":"2015","journal-title":"PLoS ONE."},{"key":"B43","doi-asserted-by":"publisher","first-page":"e0165489","DOI":"10.1371\/journal.pone.0165489","article-title":"Correction: identification of novel pre-erythrocytic malaria antigen candidates for combination vaccines with circumsporozoite protein","volume":"11","author":"Speake","year":"2016","journal-title":"PLoS ONE."},{"key":"B44","doi-asserted-by":"publisher","first-page":"e0159449","DOI":"10.1371\/journal.pone.0159449","article-title":"Identification of novel pre-erythrocytic malaria antigen candidates for combination vaccines with circumsporozoite protein","volume":"11","author":"Speake","year":"2016","journal-title":"PLoS ONE."},{"key":"B45","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1097\/00000441-197309000-00002","article-title":"Immunization of man against sporozite-induced falciparum malaria","volume":"266","author":"Clyde","year":"1973","journal-title":"Am J Med Sci."},{"key":"B46","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1038\/nature03188","article-title":"Genetically modified Plasmodium parasites as a protective experimental malaria vaccine","volume":"433","author":"Mueller","year":"2005","journal-title":"Nature."},{"key":"B47","doi-asserted-by":"publisher","first-page":"4975","DOI":"10.1016\/j.vaccine.2013.08.007","article-title":"First-in-human evaluation of genetically attenuated Plasmodium falciparum sporozoites administered by bite of Anopheles mosquitoes to adult volunteers","volume":"31","author":"Spring","year":"2013","journal-title":"Vaccine."},{"key":"B48","doi-asserted-by":"publisher","first-page":"2487","DOI":"10.4049\/jimmunol.172.4.2487","article-title":"Protective T cell immunity against malaria liver stage after vaccination with live sporozoites under chloroquine treatment","volume":"172","author":"Belnoue","year":"2004","journal-title":"J Immunol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1056\/NEJMoa0805832","article-title":"Protection against a malaria challenge by sporozoite inoculation","volume":"361","author":"Roestenberg","year":"2009","journal-title":"N Engl J Med."},{"key":"B50","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1038\/330664a0","article-title":"Gamma interferon, CD8+ T cells and antibodies required for immunity to malaria sporozoites","volume":"330","author":"Schofield","year":"1987","journal-title":"Nature."},{"key":"B51","doi-asserted-by":"publisher","first-page":"11820","DOI":"10.1038\/srep11820","article-title":"Comparative assessment of vaccine vectors encoding ten malaria antigens identifies two protective liver-stage candidates","volume":"5","author":"Longley","year":"2015","journal-title":"Sci Rep."},{"key":"B52","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1016\/j.cell.2007.09.013","article-title":"Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite","volume":"131","author":"Singh","year":"2007","journal-title":"Cell."},{"key":"B53","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1586\/erv.12.92","article-title":"Pre-erythrocytic malaria vaccines: identifying the targets","volume":"11","author":"Duffy","year":"2012","journal-title":"Expert Rev Vaccines."},{"key":"B54","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1038\/360434a0","article-title":"Molecular analysis of the association of HLA-B53 and resistance to severe malaria","volume":"360","author":"Hill","year":"1992","journal-title":"Nature."},{"key":"B55","doi-asserted-by":"publisher","first-page":"286re285","DOI":"10.1126\/scitranslmed.aaa2373","article-title":"Prime-boost vaccination with chimpanzee adenovirus and modified vaccinia Ankara encoding TRAP provides partial protection against Plasmodium falciparum infection in Kenyan adults","volume":"7","author":"Ogwang","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"B56","doi-asserted-by":"publisher","first-page":"5135","DOI":"10.1016\/j.vaccine.2009.08.046","article-title":"Recombinant liver stage antigen-1 (LSA-1) formulated with AS01 or AS02 is safe, elicits high titer antibody and induces IFN-gamma\/IL-2 CD4+ T cells but does not protect against experimental Plasmodium falciparum infection","volume":"28","author":"Cummings","year":"2010","journal-title":"Vaccine."},{"key":"B57","doi-asserted-by":"publisher","first-page":"e12294","DOI":"10.1371\/journal.pone.0012294","article-title":"Immunization with pre-erythrocytic antigen CelTOS from Plasmodium falciparum elicits cross-species protection against heterologous challenge with Plasmodium berghei","volume":"5","author":"Bergmann-Leitner","year":"2010","journal-title":"PLoS ONE."},{"key":"B58","doi-asserted-by":"publisher","first-page":"D539","DOI":"10.1093\/nar\/gkn814","article-title":"PlasmoDB: a functional genomic database for malaria parasites","volume":"37","author":"Aurrecoechea","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"B59","doi-asserted-by":"publisher","first-page":"1576","DOI":"10.1093\/infdis\/171.6.1576","article-title":"Safety, immunogenicity, and efficacy of a recombinantly produced Plasmodium falciparum circumsporozoite protein-hepatitis B surface antigen subunit vaccine","volume":"171","author":"Gordon","year":"1995","journal-title":"J Infect Dis."},{"key":"B60","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1007\/978-1-62703-026-7_27","article-title":"Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry","volume":"923","author":"Sinnis","year":"2013","journal-title":"Methods Mol Biol."},{"key":"B61","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.chom.2013.06.005","article-title":"A microscale human liver platform that supports the hepatic stages of Plasmodium falciparum and vivax","volume":"14","author":"March","year":"2013","journal-title":"Cell Host Microbe."},{"key":"B62","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1186\/1475-2875-11-421","article-title":"A rapid and scalable density gradient purification method for Plasmodium sporozoites","volume":"11","author":"Kennedy","year":"2012","journal-title":"Malar J."},{"key":"B63","doi-asserted-by":"publisher","first-page":"2908","DOI":"10.1038\/s41467-018-05374-3","article-title":"A mosquito salivary gland protein partially inhibits Plasmodium sporozoite cell traversal and transmission","volume":"9","author":"Schleicher","year":"2018","journal-title":"Nat Commun."},{"key":"B64","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1093\/milmed\/134.9.1176","article-title":"Protective immunity produced by the injection of x-irradiated sporozoites of Plasmodium berghei. IV Dose response, specificity and humoral immunity","volume":"134","author":"Nussenzweig","year":"1969","journal-title":"Mil Med."},{"key":"B65","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1186\/s12915-014-0086-0","article-title":"A comprehensive evaluation of rodent malaria parasite genomes and gene expression","volume":"12","author":"Otto","year":"2014","journal-title":"BMC Biol."},{"key":"B66","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1016\/j.ebiom.2016.04.011","article-title":"Integrated analysis of the Plasmodium species transcriptome","volume":"7","author":"Hoo","year":"2016","journal-title":"EBioMedicine."},{"key":"B67","doi-asserted-by":"publisher","first-page":"965","DOI":"10.3389\/fmicb.2015.00965","article-title":"Malaria vaccines: identifying Plasmodium falciparum liver-stage targets","volume":"6","author":"Longley","year":"2015","journal-title":"Front Microbiol."},{"key":"B68","doi-asserted-by":"publisher","first-page":"e29289","DOI":"10.1371\/journal.pone.0029289","article-title":"A novel \u2018gene insertion\/marker out\u2019 (GIMO) method for transgene expression and gene complementation in rodent malaria parasites","volume":"6","author":"Lin","year":"2011","journal-title":"PLoS ONE."},{"key":"B69","doi-asserted-by":"publisher","first-page":"e01414","DOI":"10.1128\/mBio.01414-14","article-title":"Efficient editing of malaria parasite genome using the CRISPR\/Cas9 system","volume":"5","author":"Zhang","year":"2014","journal-title":"mBio."},{"key":"B70","doi-asserted-by":"publisher","first-page":"9740","DOI":"10.1038\/s41598-017-09569-4","article-title":"A Plasmodium plasma membrane reporter reveals membrane dynamics by live-cell microscopy","volume":"7","author":"Burda","year":"2017","journal-title":"Sci Rep."},{"key":"B71","doi-asserted-by":"publisher","first-page":"eaag2490","DOI":"10.1126\/scitranslmed.aag2490","article-title":"Infectivity of Plasmodium falciparum sporozoites determines emerging parasitemia in infected volunteers","volume":"9","author":"McCall","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"B72","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.jcmgh.2017.11.005","article-title":"Engineered livers for infectious diseases","volume":"5","author":"Gural","year":"2018","journal-title":"Cell Mol Gastroenterol Hepatol."},{"key":"B73","doi-asserted-by":"publisher","first-page":"764","DOI":"10.1002\/ca.22350","article-title":"Clinical and surgical anatomy of the liver: a review for clinicians","volume":"27","author":"Juza","year":"2014","journal-title":"Clin Anat."},{"key":"B74","doi-asserted-by":"publisher","first-page":"S54","DOI":"10.1002\/hep.21060","article-title":"The liver as an immunological organ","volume":"43","author":"Racanelli","year":"2006","journal-title":"Hepatology"},{"key":"B75","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1126\/science.170.3955.340","article-title":"Fine structure of the exoerythrocytic stage of Plasmodium cynomolgi","volume":"170","author":"Sodeman","year":"1970","journal-title":"Science."},{"key":"B76","doi-asserted-by":"publisher","first-page":"682","DOI":"10.4269\/ajtmh.1983.32.682","article-title":"In vitro cultivation of the exoerythrocytic stage of Plasmodium berghei in a hepatoma cell line","volume":"32","author":"Hollingdale","year":"1983","journal-title":"Am J Trop Med Hyg."},{"key":"B77","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/j.pt.2011.09.004","article-title":"A toolbox to study liver stage malaria","volume":"27","author":"Prudencio","year":"2011","journal-title":"Trends Parasitol."},{"key":"B78","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1016\/j.stemcr.2015.01.002","article-title":"Human iPSC-derived hepatocyte-like cells support Plasmodium liver-stage infection in vitro","volume":"4","author":"Ng","year":"2015","journal-title":"Stem Cell Reports."},{"key":"B79","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1517\/17425255.2.2.183","article-title":"Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies","volume":"2","author":"Castell","year":"2006","journal-title":"Expert Opin Drug Metab Toxicol."},{"key":"B80","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2174\/138920008783331086","article-title":"Cell lines: a tool for in vitro drug metabolism studies","volume":"9","author":"Donato","year":"2008","journal-title":"Curr Drug Metab."},{"key":"B81","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1007\/s13238-015-0180-2","article-title":"In vitro culture of isolated primary hepatocytes and stem cell-derived hepatocyte-like cells for liver regeneration","volume":"6","author":"Hu","year":"2015","journal-title":"Protein Cell."},{"key":"B82","doi-asserted-by":"publisher","first-page":"21","DOI":"10.4269\/ajtmh.1985.34.21","article-title":"In vitro cultivation of the exoerythrocytic stage of Plasmodium berghei in irradiated hepatoma cells","volume":"34","author":"Hollingdale","year":"1985","journal-title":"Am J Trop Med Hyg."},{"key":"B83","doi-asserted-by":"crossref","first-page":"909","DOI":"10.4049\/jimmunol.132.2.909","article-title":"Inhibition of entry of Plasmodium falciparum and P. vivax sporozoites into cultured cells; an in vitro assay of protective antibodies","volume":"132","author":"Hollingdale","year":"1984","journal-title":"J Immunol."},{"key":"B84","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.4049\/jimmunol.139.6.2020","article-title":"Interferon-gamma inhibits the intrahepatocytic development of malaria parasites in vitro","volume":"139","author":"Schofield","year":"1987","journal-title":"J Immunol."},{"key":"B85","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/0014-4894(77)90063-7","article-title":"Plasmodium berghei: T cell dependence of sporozoite-induced immunity in rodents","volume":"42","author":"Spitalny","year":"1977","journal-title":"Exp Parasitol."},{"key":"B86","doi-asserted-by":"publisher","first-page":"17011","DOI":"10.1038\/s41598-017-17274-5","article-title":"An in vitro assay to measure antibody-mediated inhibition of P. berghei sporozoite invasion against P. falciparum antigens","volume":"7","author":"Rodriguez-Galan","year":"2017","journal-title":"Sci Rep."},{"key":"B87","doi-asserted-by":"publisher","first-page":"3628","DOI":"10.1128\/IAI.00471-08","article-title":"Effector CD8+ T lymphocytes against liver stages of Plasmodium yoelii do not require gamma interferon for antiparasite activity","volume":"76","author":"Chakravarty","year":"2008","journal-title":"Infect Immun."},{"key":"B88","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.4049\/jimmunol.156.9.3374","article-title":"MHC class I-dependent presentation of exoerythrocytic antigens to CD8+ T lymphocytes is required for protective immunity against Plasmodium berghei","volume":"156","author":"White","year":"1996","journal-title":"J Immunol."},{"key":"B89","first-page":"115","article-title":"The development and routine application of high-density exoerythrocytic-stage cultures of Plasmodium berghei","volume":"68","author":"Sinden","year":"1990","journal-title":"Bull World Health Organ."},{"key":"B90","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1084\/jem.194.2.173","article-title":"Swift development of protective effector functions in naive CD8(+) T cells against malaria liver stages","volume":"194","author":"Sano","year":"2001","journal-title":"J Exp Med."},{"key":"B91","doi-asserted-by":"publisher","first-page":"e1002401","DOI":"10.1371\/journal.ppat.1002401","article-title":"The role of animal models for research on severe malaria","volume":"8","author":"Craig","year":"2012","journal-title":"PLoS Pathog."},{"key":"B92","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1038\/90968","article-title":"Hepatitis C virus replication in mice with chimeric human livers","volume":"7","author":"Mercer","year":"2001","journal-title":"Nat Med."},{"key":"B93","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/j.ijpara.2005.10.014","article-title":"Plasmodium falciparum infection and exoerythrocytic development in mice with chimeric human livers","volume":"36","author":"Sacci","year":"2006","journal-title":"Int. J. Parasitol."},{"key":"B94","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1038\/nbt1326","article-title":"Robust expansion of human hepatocytes in Fah-\/-\/Rag2-\/-\/Il2rg-\/- mice","volume":"25","author":"Azuma","year":"2007","journal-title":"Nat Biotechnol."},{"key":"B95","doi-asserted-by":"publisher","first-page":"3618","DOI":"10.1172\/JCI62684","article-title":"Complete Plasmodium falciparum liver-stage development in liver-chimeric mice","volume":"122","author":"Vaughan","year":"2012","journal-title":"J Clin Invest."},{"key":"B96","doi-asserted-by":"publisher","first-page":"7690","DOI":"10.1038\/ncomms8690","article-title":"Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice","volume":"6","author":"Soulard","year":"2015","journal-title":"Nat Commun."},{"key":"B97","doi-asserted-by":"publisher","first-page":"45424","DOI":"10.1038\/srep45424","article-title":"Towards a Humanized mouse model of liver stage malaria using ectopic artificial livers","volume":"7","author":"Ng","year":"2017","journal-title":"Sci Rep."},{"key":"B98","doi-asserted-by":"publisher","first-page":"386","DOI":"10.1186\/1475-2875-13-386","article-title":"Humanized HLA-DR4","volume":"13","author":"Wijayalath","year":"2014","journal-title":"Malar. J."},{"key":"B99","doi-asserted-by":"publisher","first-page":"272","DOI":"10.3389\/fmicb.2014.00272","article-title":"CD8 T-cell-mediated protection against liver-stage malaria: lessons from a mouse model","volume":"5","author":"Van Braeckel-Budimir","year":"2014","journal-title":"Front Microbiol."},{"key":"B100","doi-asserted-by":"publisher","first-page":"e0119880","DOI":"10.1371\/journal.pone.0119880","article-title":"Development of an in vitro assay and demonstration of Plasmodium berghei liver-stage inhibition by TRAP-specific CD8+ T cells","volume":"10","author":"Longley","year":"2015","journal-title":"PLoS ONE."},{"key":"B101","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1111\/j.1462-5822.2007.01032.x","article-title":"Dissecting in vitro host cell infection by Plasmodium sporozoites using flow cytometry","volume":"10","author":"Prudencio","year":"2008","journal-title":"Cell Microbiol."},{"key":"B102","doi-asserted-by":"publisher","first-page":"708","DOI":"10.4269\/ajtmh.2006.74.708","article-title":"Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax","volume":"74","author":"Sattabongkot","year":"2006","journal-title":"Am J Trop Med Hyg."},{"key":"B103","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1242\/dmm.013490","article-title":"Hypoxia promotes liver-stage malaria infection in primary human hepatocytes in vitro","volume":"7","author":"Ng","year":"2014","journal-title":"Dis Model Mech."},{"key":"B104","doi-asserted-by":"publisher","first-page":"e75321","DOI":"10.1371\/journal.pone.0075321","article-title":"Dynamics of the major histocompatibility complex class I processing and presentation pathway in the course of malaria parasite development in human hepatocytes: implications for vaccine development","volume":"8","author":"Ma","year":"2013","journal-title":"PLoS ONE."},{"key":"B105","doi-asserted-by":"publisher","first-page":"e17464","DOI":"10.1371\/journal.pone.0017464","article-title":"Inhibitory effect of TNF-alpha on malaria pre-erythrocytic stage development: influence of host hepatocyte\/parasite combinations","volume":"6","author":"Depinay","year":"2011","journal-title":"PLoS ONE."},{"key":"B106","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1006\/expr.2000.4570","article-title":"Plasmodium yoelii: efficient in vitro invasion and complete development of sporozoites in mouse hepatic cell lines","volume":"96","author":"Mota","year":"2000","journal-title":"Exp Parasitol."},{"key":"B107","doi-asserted-by":"publisher","first-page":"e0129623","DOI":"10.1371\/journal.pone.0129623","article-title":"Flow cytometry based detection and isolation of Plasmodium falciparum liver stages in vitro","volume":"10","author":"Dumoulin","year":"2015","journal-title":"PLoS ONE."},{"key":"B108","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.molbiopara.2012.01.006","article-title":"Development of a quantitative flow cytometry-based assay to assess infection by Plasmodium falciparum sporozoites","volume":"183","author":"Kaushansky","year":"2012","journal-title":"Mol Biochem Parasitol."},{"key":"B109","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/nm808","article-title":"Hepatocyte CD81 is required for Plasmodium falciparum and Plasmodium yoelii sporozoite infectivity","volume":"9","author":"Silvie","year":"2002","journal-title":"Nat Med."},{"key":"B110","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1126\/science.aad3318","article-title":"Malaria parasites target the hepatocyte receptor EphA2 for successful host infection","volume":"350","author":"Kaushansky","year":"2015","journal-title":"Science."},{"key":"B111","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/j.chom.2008.07.012","article-title":"Host scavenger receptor SR-BI plays a dual role in the establishment of malaria parasite liver infection","volume":"4","author":"Rodrigues","year":"2008","journal-title":"Cell Host Microbe."},{"key":"B112","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1111\/imm.12780","article-title":"Vaccine candidate discovery for the next generation of malaria vaccines","volume":"152","author":"Tuju","year":"2017","journal-title":"Immunology."},{"key":"B113","doi-asserted-by":"publisher","first-page":"E5","DOI":"10.1371\/journal.pbio.0000005","article-title":"The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum","volume":"1","author":"Bozdech","year":"2003","journal-title":"PLoS Biol."},{"key":"B114","doi-asserted-by":"publisher","first-page":"1503","DOI":"10.1126\/science.1087025","article-title":"Discovery of gene function by expression profiling of the malaria parasite life cycle","volume":"301","author":"Le","year":"2003","journal-title":"Science."},{"key":"B115","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1126\/science.1103717","article-title":"A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analyses","volume":"307","author":"Hall","year":"2005","journal-title":"Science."},{"key":"B116","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1093\/bfgp\/ely013","article-title":"Host-parasite interactions and ecology of the malaria parasite-a bioinformatics approach","volume":"17","author":"Izak","year":"2018","journal-title":"Brief Funct Genomics."},{"key":"B117","doi-asserted-by":"publisher","first-page":"1816","DOI":"10.1126\/science.287.5459.1816","article-title":"Identification of vaccine candidates against serogroup B meningococcus by whole-genome sequencing","volume":"287","author":"Pizza","year":"2000","journal-title":"Science."},{"key":"B118","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1126\/science.1109869","article-title":"Identification of a universal Group B streptococcus vaccine by multiple genome screen","volume":"309","author":"Maione","year":"2005","journal-title":"Science."},{"key":"B119","doi-asserted-by":"publisher","first-page":"2252","DOI":"10.1016\/j.vaccine.2006.12.003","article-title":"Discovery of a vaccine antigen that protects mice from Chlamydia pneumoniae infection","volume":"25","author":"Thorpe","year":"2007","journal-title":"Vaccine."},{"key":"B120","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1084\/jem.20071168","article-title":"Discovery of a novel class of highly conserved vaccine antigens using genomic scale antigenic fingerprinting of pneumococcus with human antibodies","volume":"205","author":"Giefing","year":"2008","journal-title":"J Exp Med."},{"key":"B121","doi-asserted-by":"publisher","first-page":"6817","DOI":"10.1128\/IAI.70.12.6817-6827.2002","article-title":"Search for potential vaccine candidate open reading frames in the Bacillus anthracis virulence plasmid pXO1: in silico and in vitro screening","volume":"70","author":"Ariel","year":"2002","journal-title":"Infect Immun."},{"key":"B122","doi-asserted-by":"publisher","first-page":"4135","DOI":"10.1016\/S0264-410X(01)00173-6","article-title":"Identification of vaccine candidate antigens from a genomic analysis of Porphyromonas gingivalis","volume":"19","author":"Ross","year":"2001","journal-title":"Vaccine."},{"key":"B123","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1038\/ng1924","article-title":"Genome-wide variation and identification of vaccine targets in the Plasmodium falciparum genome","volume":"39","author":"Mu","year":"2007","journal-title":"Nat Genet."},{"key":"B124","doi-asserted-by":"publisher","first-page":"4203","DOI":"10.1016\/j.vaccine.2007.03.012","article-title":"Phase I dose escalation safety and immunogenicity trial of Plasmodium falciparum apical membrane protein (AMA-1) FMP2.1, adjuvanted with AS02A, in malaria-na\u00efve adults at the Walter Reed Army Institute of Research","volume":"5","author":"Polhemus","year":"2007","journal-title":"Vaccine."},{"key":"B125","doi-asserted-by":"publisher","first-page":"2769","DOI":"10.3389\/fimmu.2018.02769","article-title":"Novel strategies for malaria vaccine design","volume":"9","author":"Frimpong","year":"2018","journal-title":"Front Immunol."},{"key":"B126","doi-asserted-by":"publisher","first-page":"3105","DOI":"10.1074\/mcp.O115.052431","article-title":"Analysis of major histocompatibility complex (MHC) immunopeptidomes using mass spectrometry","volume":"14","author":"Caron","year":"2015","journal-title":"Mol Cell Proteomics."},{"key":"B127","doi-asserted-by":"publisher","first-page":"2612","DOI":"10.1111\/febs.13287","article-title":"Proteomics beyond trypsin","volume":"282","author":"Tsiatsiani","year":"2015","journal-title":"FEBS J."},{"key":"B128","doi-asserted-by":"publisher","first-page":"310ra167","DOI":"10.1126\/scitranslmed.aac5477","article-title":"Identification of broadly conserved cross-species protective Leishmania antigen and its responding CD4+ T cells","volume":"7","author":"Mou","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"B129","doi-asserted-by":"publisher","first-page":"1605","DOI":"10.15252\/emmm.201708123","article-title":"Profiling MHC II immunopeptidome of blood-stage malaria reveals that cDC1 control the functionality of parasite-specific CD4 T cells","volume":"9","author":"Draheim","year":"2017","journal-title":"EMBO Mol Med."},{"key":"B130","doi-asserted-by":"publisher","first-page":"663","DOI":"10.4269\/ajtmh.15-0621","article-title":"Safety, immunogenicity, and protective efficacy of intradermal immunization with aseptic, purified, cryopreserved Plasmodium falciparum sporozoites in volunteers under chloroquine prophylaxis: a randomized controlled trial","volume":"94","author":"Bastiaens","year":"2016","journal-title":"Am J Trop Med Hyg."},{"key":"B131","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1038\/ncomms1615","article-title":"The blood-stage malaria antigen PfRH5 is susceptible to vaccine-inducible cross-strain neutralizing antibody","volume":"2","author":"Douglas","year":"2011","journal-title":"Nat Commun."},{"key":"B132","doi-asserted-by":"publisher","first-page":"7444","DOI":"10.1016\/j.vaccine.2015.09.094","article-title":"Progress with viral vectored malaria vaccines: a multi-stage approach involving \u201cunnatural immunity\u201d","volume":"33","author":"Ewer","year":"2015","journal-title":"Vaccine."},{"key":"B133","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.chom.2015.01.014","article-title":"A genome-scale vector resource enables high-throughput reverse genetic screening in a malaria parasite","volume":"17","author":"Gomes","year":"2015","journal-title":"Cell Host Microbe."},{"key":"B134","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/j.jmgm.2017.10.004","article-title":"In silico design of knowledge-based Plasmodium falciparum epitope ensemble vaccines","volume":"78","author":"Damfo","year":"2017","journal-title":"J Mol Graph Model."},{"key":"B135","doi-asserted-by":"publisher","first-page":"7496","DOI":"10.1016\/j.vaccine.2015.09.059","article-title":"Large screen approaches to identify novel malaria vaccine candidates","volume":"33","author":"Davies","year":"2015","journal-title":"Vaccine."}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2020.00190\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T09:15:47Z","timestamp":1695806147000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2020.00190\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,21]]},"references-count":135,"alternative-id":["10.3389\/fimmu.2020.00190"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2020.00190","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,21]]},"article-number":"190"}}