{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T10:00:17Z","timestamp":1792058417621,"version":"4.4.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T00:00:00Z","timestamp":1588809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2020.00655","type":"journal-article","created":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T15:43:12Z","timestamp":1588866192000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":58,"title":["Development of Nanobodies Against Hemorrhagic and Myotoxic Components of Bothrops atrox Snake Venom"],"prefix":"10.3389","volume":"11","author":[{"given":"Henri","family":"Bailon Calderon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica Olga","family":"Yaniro Coronel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar Alberto","family":"C\u00e1ceres Rey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Gaby","family":"Colque Alave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter Jhon","family":"Leiva Duran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Padilla Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harrison","family":"Montejo Arevalo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Garc\u00eda Neyra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Galarza P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar","family":"Bonilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benigno","family":"Tintaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Ricciardi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Smiejkowska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ema","family":"Rom\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Vincke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"L\u00e9vano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Celys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Lomonte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Muyldermans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,5,7]]},"reference":[{"key":"B1","year":"2018","journal-title":"Snake Antivenoms."},{"key":"B2","doi-asserted-by":"publisher","first-page":"6617","DOI":"10.2741\/3177","article-title":"Disintegrins in health and disease.","volume":"13","author":"McLane","year":"2008","journal-title":"Front Biosci."},{"key":"B3","first-page":"211","article-title":"Local tissue damage induced by Bothrops snake venoms. A review.","volume":"51","author":"Guti\u00e9rrez","year":"1989","journal-title":"Mem Inst Butantan."},{"key":"B4","doi-asserted-by":"publisher","first-page":"721","DOI":"10.2165\/00003088-200342080-00002","article-title":"Pharmacokinetic-pharmacodynamic relationships of immunoglobulin therapy for envenomation.","volume":"42","author":"Guti\u00e9rrez","year":"2003","journal-title":"Clin Pharmacokinet."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.2217\/nnm.13.86","article-title":"Nanobodies and their potential applications.","volume":"8","author":"Hassanzadeh-Ghassabeh","year":"2013","journal-title":"Nanomedicine."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1126","DOI":"10.1038\/nbt1142","article-title":"Engineered antibody fragments and the rise of single domains.","volume":"23","author":"Holliger","year":"2005","journal-title":"Nat Biotechnol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/s0968-0004(01)01790-x","article-title":"Recognition of antigens by single-domain antibody fragments: the superfluous luxury of paired domains.","volume":"26","author":"Muyldermans","year":"2001","journal-title":"Trends Biochem Sci."},{"key":"B8","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1146\/annurev-biochem-063011-092449","article-title":"Nanobodies: natural single-domain antibodies.","volume":"82","author":"Muyldermans","year":"2013","journal-title":"Annu Rev Biochem."},{"key":"B9","doi-asserted-by":"publisher","first-page":"2013","DOI":"10.1002\/ijc.26145","article-title":"A biparatopic anti-EGFR nanobody efficiently inhibits solid tumour growth.","volume":"129","author":"Roovers","year":"2011","journal-title":"Int J Cancer."},{"key":"B10","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.toxicon.2005.10.017","article-title":"Neutralisation of venom-induced haemorrhage by IgG from camels and llamas immunised with viper venom and also by endogenous, non-IgG components in camelid sera.","volume":"47","author":"Harrison","year":"2006","journal-title":"Toxicon."},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.3390\/toxins10040142","article-title":"Camelid single-domain antibodies (VHHs) against crotoxin: a basis for developing modular building blocks for the enhancement of treatment or diagnosis of crotalic envenoming.","volume":"10","author":"Luiz","year":"2018","journal-title":"Toxins."},{"key":"B12","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1016\/j.ijpara.2008.10.012","article-title":"Nanobodies, a promising tool for species-specific diagnosis of Taenia","volume":"39","author":"Deckers","year":"2009","journal-title":"Int J Parasitol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1970","DOI":"10.1074\/jbc.M111.242818","article-title":"Dual beneficial effect of interloop disulfide bond for single domain antibody fragments.","volume":"287","author":"Govaert","year":"2012","journal-title":"J Biol Chem."},{"key":"B14","doi-asserted-by":"publisher","first-page":"4586","DOI":"10.1073\/pnas.0505379103","article-title":"Molecular basis for the preferential cleft recognition by dromedary heavy-chain antibodies.","volume":"103","author":"De Genst","year":"2006","journal-title":"Proc Natl Acad Sci USA."},{"key":"B15","doi-asserted-by":"publisher","first-page":"4501","DOI":"10.1021\/ac058044j","article-title":"Isolation and characterization of a thermally stable recombinant anti-caffeine heavy-chain antibody fragment.","volume":"78","author":"Ladenson","year":"2006","journal-title":"Anal Chem."},{"key":"B16","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.toxicon.2015.09.007","article-title":"Partial in","volume":"108","author":"Guerra-Duarte","year":"2015","journal-title":"Toxicon."},{"key":"B17","doi-asserted-by":"publisher","first-page":"2181","DOI":"10.1016\/j.jprot.2012.01.020","article-title":"barnetti and B.","volume":"75","author":"Kohlhoff","year":"2012","journal-title":"J Proteomics."},{"key":"B18","doi-asserted-by":"publisher","first-page":"40528","DOI":"10.1074\/jbc.M202049200","article-title":"The reprolysin Jararhagin, a snake venom metalloproteinase, functions as a fibrillar collagen agonist involved in fibroblast cell adhesion and signaling.","volume":"277","author":"Zigrino","year":"2002","journal-title":"J Biol Chem."},{"key":"B19","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1016\/j.toxicon.2007.08.005","article-title":"BnP1, a novel P-I metalloproteinase from Bothrops","volume":"51","author":"Baldo","year":"2008","journal-title":"Toxicon."},{"key":"B20","first-page":"647","article-title":"Phospholipases A2 from viperidae snake venoms: how do they induce skeletal muscle damage?","volume":"58","author":"Lomonte","year":"2011","journal-title":"Acta Chim Slov."},{"key":"B21","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.toxicon.2012.09.006","article-title":"Phospholipases A2: unveiling the secrets of a functionally versatile group of snake venom toxins.","volume":"62","author":"Guti\u00e9rrez","year":"2013","journal-title":"Toxicon."},{"key":"B22","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.toxicon.2018.03.004","article-title":"Pros and cons of different therapeutic antibody formats for recombinant antivenom development.","volume":"146","author":"Laustsen","year":"2018","journal-title":"Toxicon."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005361","article-title":"Recombinant snakebite antivenoms: a cost-competitive solution to a neglected tropical disease?","volume":"11","author":"Laustsen","year":"2017","journal-title":"PLoS Negl Trop Dis."},{"key":"B24","doi-asserted-by":"publisher","first-page":"43","DOI":"10.7883\/yoken1952.13.43","article-title":"Studies on the quantitative method for determination of hemorrhagic activity of habu snake venom.","volume":"13","author":"Kondo","year":"1960","journal-title":"Jpn J Med Sci Biol."},{"key":"B25","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/0041-0101(82)90051-4","article-title":"Neutralization of local effects of the terciopelo (Bothrops asper) venom by blood serum of the colubrid snake Clelia clelia.","volume":"20","author":"Lomonte","year":"1982","journal-title":"Toxicon."},{"key":"B26","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/0041-0101(80)90087-2","article-title":"Mionecrosis, hemorragia y edema inducidos por el veneno de Bothrops","volume":"18","author":"Guti\u00e9rrez","year":"1980","journal-title":"Toxicon."},{"key":"B27","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0041-0101(00)00122-7","article-title":"The venom of Bothrops","volume":"39","author":"Saravia","year":"2001","journal-title":"Toxicon."},{"key":"B28","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.toxicon.2017.10.005","article-title":"An improved technique for the assessment of venom-induced haemorrhage in a murine model.","volume":"139","author":"Jenkins","year":"2017","journal-title":"Toxicon."},{"key":"B29","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.toxicon.2004.04.013","article-title":"Structural and functional characterization of myotoxin I, a Lys49 phospholipase A2 homologue from the venom of the snake Bothrops","volume":"44","author":"N\u00fa\u00f1ez","year":"2004","journal-title":"Toxicon."},{"key":"B30","doi-asserted-by":"publisher","first-page":"860","DOI":"10.2174\/092986609788923356","article-title":"The Phospholipase A2 homologues of snake venoms: biological activities and their possible adaptive roles.","volume":"16","author":"Lomonte","year":"2009","journal-title":"Protein Pept Lett."},{"key":"B31","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1016\/j.toxicon.2005.02.029","article-title":"Hemorrhage induced by snake venom metalloproteinases: biochemical and biophysical mechanisms involved in microvessel damage.","volume":"45","author":"Guti\u00e9rrez","year":"2005","journal-title":"Toxicon."},{"key":"B32","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.toxicon.2014.11.223","article-title":"The lethality test used for estimating the potency of antivenoms against Bothrops","volume":"93","author":"Chac\u00f3n","year":"2015","journal-title":"Toxicon."},{"key":"B33","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1016\/j.toxicon.2006.11.023","article-title":"Isolation, characterization and pentamerization of \u03b1-cobrotoxin specific single-domain antibodies from a na\u00efve phage display library: preliminary findings for antivenom development.","volume":"49","author":"Stewart","year":"2007","journal-title":"Toxicon."},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0151363","article-title":"Inhibition of the myotoxicity induced by Bothrops","volume":"11","author":"Prado","year":"2016","journal-title":"PLoS One."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2541","DOI":"10.3390\/toxins6082541","article-title":"Engineering Venom\u2019s toxin-neutralizing antibody fragments and its therapeutic potential.","volume":"6","author":"Alvarenga","year":"2014","journal-title":"Toxins (Basel)."},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06086-4","article-title":"In vivo neutralization of dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG antibodies.","volume":"9","author":"Laustsen","year":"2018","journal-title":"Nat Commun."},{"key":"B37","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.toxicon.2019.06.005","article-title":"Engineering and design considerations for next-generation snakebite antivenoms.","volume":"167","author":"Knudsen","year":"2019","journal-title":"Toxicon."}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2020.00655\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T15:43:14Z","timestamp":1588866194000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2020.00655\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,7]]},"references-count":37,"alternative-id":["10.3389\/fimmu.2020.00655"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2020.00655","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,7]]},"article-number":"655"}}