{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T18:22:56Z","timestamp":1787077376984,"version":"build-2736575974"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2020.01659","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T04:49:28Z","timestamp":1596516568000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Conventional and Computational Flow Cytometry Analyses Reveal Sustained Human Intrathymic T Cell Development From Birth Until Puberty"],"prefix":"10.3389","volume":"11","author":[{"given":"Marieke","family":"Lavaert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brecht","family":"Valcke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart","family":"Vandekerckhove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges","family":"Leclercq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Ling","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Taghon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,8,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1016\/j.it.2009.05.001","article-title":"An evolutionary perspective on the mechanisms of immunosenescence","volume":"30","author":"Shanley","year":"2009","journal-title":"Trends Immunol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1007\/s10522-011-9349-0","article-title":"It's not all equal: a multiphasic theory of thymic involution","volume":"13","author":"Aw","year":"2012","journal-title":"Biogerontology."},{"key":"B3","doi-asserted-by":"publisher","first-page":"646","DOI":"10.3324\/haematol.2010.036343","article-title":"T-lymphoid differentiation potential measured in vitro is higher in CD34+CD38-\/lo hematopoietic stem cells from umbilical cord blood than from bone marrow and is an intrinsic property of the cells","volume":"96","author":"De Smedt","year":"2011","journal-title":"Haematologica."},{"key":"B4","doi-asserted-by":"publisher","first-page":"245","DOI":"10.4049\/jimmunol.173.1.245","article-title":"Reduction in the developmental potential of intrathymic T cell progenitors with age","volume":"173","author":"Min","year":"2004","journal-title":"J Immunol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1046\/j.1365-2141.1999.01265.x","article-title":"Bone marrow CD34 cells generate fewer T cells in vitro with increasing age and following chemotherapy","volume":"104","author":"Offner","year":"1999","journal-title":"Br J Haematol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1242\/dev.103614","article-title":"Regeneration of the aged thymus by a single transcription factor","volume":"141","author":"Bredenkamp","year":"2014","journal-title":"Development."},{"key":"B7","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1182\/blood-2008-05-156265","article-title":"Foxn1 is required to maintain the postnatal thymic microenvironment in a dosage-sensitive manner","volume":"113","author":"Chen","year":"2009","journal-title":"Blood."},{"key":"B8","doi-asserted-by":"publisher","first-page":"3887","DOI":"10.1016\/j.celrep.2019.05.045","article-title":"Interplay between follistatin, activin A, BMP4 signaling regulates postnatal thymic epithelial progenitor cell differentiation during aging","volume":"27","author":"Lepletier","year":"2019","journal-title":"Cell Rep."},{"key":"B9","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.4049\/jimmunol.142.10.3369","article-title":"Cell proliferation and differentiation in the fetal and early postnatal mouse thymus","volume":"142","author":"Penit","year":"1989","journal-title":"J Immunol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1093\/intimm\/dxf042","article-title":"Molecular characterization of the mouse involuted thymus: aberrations in expression of transcription regulators in thymocyte and epithelial compartments","volume":"14","author":"Ortman","year":"2002","journal-title":"Int Immunol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1016\/S0161-5890(01)00122-5","article-title":"T cell receptor excision circle assessment of thymopoiesis in aging mice","volume":"38","author":"Sempowski","year":"2002","journal-title":"Mol Immunol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.cellimm.2007.10.007","article-title":"The role of sex steroids and gonadectomy in the control of thymic involution","volume":"252","author":"Hince","year":"2008","journal-title":"Cell Immunol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"6260","DOI":"10.4049\/jimmunol.164.12.6260","article-title":"Analysis of the human neonatal thymus: evidence for a transient thymic involution","volume":"164","author":"Varas","year":"2000","journal-title":"J Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"834","DOI":"10.1016\/j.jaci.2004.10.031","article-title":"Age-related changes in the cellular composition of the thymus in children","volume":"115","author":"Weerkamp","year":"2005","journal-title":"J Allergy Clin Immunol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1111\/j.1365-3083.1985.tb01916.x","article-title":"The involution of the ageing human thymic epithelium is independent of puberty. A morphometric study","volume":"22","author":"Steinmann","year":"1985","journal-title":"Scand J Immunol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"2741","DOI":"10.4049\/jimmunol.175.4.2741","article-title":"Activation of thymic regeneration in mice humans following androgen blockade","volume":"175","author":"Sutherland","year":"2005","journal-title":"J Immunol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1138","DOI":"10.1158\/1078-0432.CCR-07-1784","article-title":"Enhanced immune system regeneration in humans following allogeneic or autologous hemopoietic stem cell transplantation by temporary sex steroid blockade","volume":"14","author":"Sutherland","year":"2008","journal-title":"Clin Cancer Res."},{"key":"B18","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1016\/j.lfs.2015.10.025","article-title":"Men and mice: relating their ages","volume":"152","author":"Dutta","year":"2016","journal-title":"Life Sci."},{"key":"B19","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s13059-017-1382-0","article-title":"SCANPY: large-scale single-cell gene expression data analysis","volume":"19","author":"Wolf","year":"2018","journal-title":"Genome Biol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.cels.2019.05.003","article-title":"Geometric sketching compactly summarizes the single-cell transcriptomic landscape","volume":"8","author":"Hie","year":"2019","journal-title":"Cell Syst."},{"key":"B21","doi-asserted-by":"publisher","first-page":"5233","DOI":"10.1038\/s41598-019-41695-z","article-title":"From Louvain to Leiden: guaranteeing well-connected communities","volume":"9","author":"Traag","year":"2019","journal-title":"Sci Rep."},{"key":"B22","volume-title":"UMAP: Uniform Manifold Approximation and Projection for Dimension Reduction. ArXiv (preprint).","author":"McInnes","year":"2018"},{"key":"B23","doi-asserted-by":"publisher","first-page":"336","DOI":"10.1002\/cyto.a.23080","article-title":"A flowcytometric analysis to efficiently quantify multiple innate immune cells and T Cell subsets in human blood","volume":"91","author":"Draxler","year":"2017","journal-title":"Cytometry"},{"key":"B24","doi-asserted-by":"publisher","first-page":"e0162242","DOI":"10.1371\/journal.pone.0162242","article-title":"Comparison of human neonatal and adult blood leukocyte subset composition phenotypes","volume":"11","author":"Prabhu","year":"2016","journal-title":"PLoS ONE."},{"key":"B25","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.cell.2015.05.047","article-title":"Data-driven phenotypic dissection of AML reveals progenitor-like cells that correlate with prognosis","volume":"162","author":"Levine","year":"2015","journal-title":"Cell."},{"key":"B26","doi-asserted-by":"publisher","first-page":"636","DOI":"10.1002\/cyto.a.22625","article-title":"FlowSOM: using self-organizing maps for visualization and interpretation of cytometry data","volume":"87","author":"Van Gassen","year":"2015","journal-title":"Cytometry"},{"key":"B27","doi-asserted-by":"publisher","first-page":"eaah4447","DOI":"10.1126\/sciimmunol.aah4447","article-title":"The human thymus perivascular space is a functional niche for viral-specific plasma cells","volume":"1","author":"Nunez","year":"2016","journal-title":"Sci Immunol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"eaay3224","DOI":"10.1126\/science.aay3224","article-title":"A cell atlas of human thymic development defines T cell repertoire formation","volume":"367","author":"Park","year":"2020","journal-title":"Science."},{"key":"B29","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.immuni.2006.01.008","article-title":"Dynamics of thymus-colonizing cells during human development","volume":"24","author":"Haddad","year":"2006","journal-title":"Immunity."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1318","DOI":"10.1182\/blood-2007-08-106294","article-title":"Human intrathymic lineage commitment is marked by differential CD7 expression: identification of CD7- lympho-myeloid thymic progenitors","volume":"111","author":"Hao","year":"2008","journal-title":"Blood."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1088","DOI":"10.1016\/j.immuni.2020.03.019","article-title":"Integrated scRNA-Seq identifies human postnatal thymus seeding progenitors and regulatory dynamics of differentiating immature thymocytes","volume":"52","author":"Lavaert","year":"2020","journal-title":"Immunity."},{"key":"B32","doi-asserted-by":"publisher","first-page":"3085","DOI":"10.1084\/jem.20071003","article-title":"A human postnatal lymphoid progenitor capable of circulating and seeding the thymus","volume":"204","author":"Six","year":"2007","journal-title":"J Exp Med."},{"key":"B33","doi-asserted-by":"publisher","first-page":"3361","DOI":"10.1084\/jem.20161564","article-title":"Spatially restricted JAG1-Notch signaling in human thymus provides suitable DC developmental niches","volume":"214","author":"Mart\u00edn-Gayo","year":"2017","journal-title":"J Exp Med."},{"key":"B34","doi-asserted-by":"publisher","first-page":"32","DOI":"10.3389\/fimmu.2017.00032","article-title":"Loss of CD44(dim) expression from early progenitor cells marks T-cell lineage commitment in the human thymus","volume":"8","author":"Cant\u00e9-Barrett","year":"2017","journal-title":"Front Immunol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1038\/nbt.2594","article-title":"viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia","volume":"31","author":"Amir el","year":"2013","journal-title":"Nat Biotechnol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1038\/nmeth.4237","article-title":"Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids","volume":"14","author":"Seet","year":"2017","journal-title":"Nat Methods."},{"key":"B37","doi-asserted-by":"crossref","first-page":"3730","DOI":"10.4049\/jimmunol.158.8.3730","article-title":"MHC class II molecules are required for initiation of positive selection but not during terminal differentiation of human CD4 single positive thymocytes","volume":"158","author":"Vanhecke","year":"1997","journal-title":"J Immunol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"e47","DOI":"10.1093\/nar\/gkv007","article-title":"limma powers differential expression analyses for RNA-sequencing and microarray studies","volume":"43","author":"Ritchie","year":"2015","journal-title":"Nucleic Acids Res."}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2020.01659\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T07:20:55Z","timestamp":1696490455000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fimmu.2020.01659\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,4]]},"references-count":38,"alternative-id":["10.3389\/fimmu.2020.01659"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2020.01659","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,4]]},"article-number":"1659"}}