{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:30:31Z","timestamp":1783625431570,"version":"3.55.0"},"reference-count":65,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R33AI102264, R21AI140499, R01AI131662"],"award-info":[{"award-number":["R33AI102264, R21AI140499, R01AI131662"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Kinase activity plays an essential role in the regulation of immune cell defenses against pathogens. The protein kinase CK2 (formerly casein kinase II) is an evolutionarily conserved kinase with hundreds of identified substrates. CK2 is ubiquitously expressed in somatic and immune cells, but the roles of CK2 in regulation of immune cell function remain largely elusive. This reflects the essential role of CK2 in organismal development and limited prior work with conditional CK2 mutant murine models. Here, we generated mice with a conditional (floxed) allele of <jats:italic>Csnk2a<\/jats:italic>, which encodes the catalytic CK2\u03b1 subunit of CK2. When crossed to <jats:italic>Lyz2<\/jats:italic>-cre mice, excision of <jats:italic>Csnk2a<\/jats:italic> sequence impaired CK2\u03b1 expression in myeloid cells but failed to detectably alter myeloid cell development. By contrast, deficiency for CK2\u03b1 increased inflammatory myeloid cell recruitment, activation, and resistance following systemic <jats:italic>Listeria monocytogenes<\/jats:italic> (Lm) infection. Results from mixed chimera experiments indicated that CK2\u03b1 deficiency in only a subset of myeloid cells was not sufficient to reduce bacterial burdens. Nor did cell-intrinsic deficiency for CK2\u03b1 suffice to alter accumulation or activation of monocytes and neutrophils in infected tissues. These data suggest that CK2\u03b1 expression by <jats:italic>Lyz2<\/jats:italic>-expressing cells promotes inflammatory and anti-bacterial responses through effects <jats:italic>in trans<\/jats:italic>. Our results highlight previously undescribed suppressive effects of CK2 activity on inflammatory myeloid cell responses and illustrate that cell-extrinsic effects of CK2 can shape inflammatory and protective innate immune responses.<\/jats:p>","DOI":"10.3389\/fimmu.2020.590266","type":"journal-article","created":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T05:38:46Z","timestamp":1607405926000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection"],"prefix":"10.3389","volume":"11","author":[{"given":"Sandy R.","family":"Larson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikki","family":"Bortell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alysha","family":"Illies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Crisler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurel L.","family":"Lenz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410000256","article-title":"Subcellular localization of protein kinase CK2. 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