{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:21:44Z","timestamp":1792156904587,"version":"4.4.0"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T00:00:00Z","timestamp":1604448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016302","name":"Coalition for Epidemic Preparedness Innovations","doi-asserted-by":"publisher","award":["Platform Technology for Disease X Response"],"award-info":[{"award-number":["Platform Technology for Disease X Response"]}],"id":[{"id":"10.13039\/100016302","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"DOI":"10.3389\/fimmu.2020.592370","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T05:23:08Z","timestamp":1604467388000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":19,"title":["Rapid Response Subunit Vaccine Design in the Absence of Structural Information"],"prefix":"10.3389","volume":"11","author":[{"given":"Danushka K.","family":"Wijesundara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Avumegah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Lackenby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naphak","family":"Modhiran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel","family":"Isaacs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Watterson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith J.","family":"Chappell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,11,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1016\/j.virol.2015.03.043","article-title":"Viral membrane fusion","author":"Harrison","year":"2015","journal-title":"Virology"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1205669","article-title":"A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins","volume":"333","author":"Corti","year":"2011","journal-title":"Science"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115941109","article-title":"Neutralizing antibodies against the preactive form of respiratory syncytial virus fusion protein offer unique possibilities for clinical intervention","volume":"109","author":"Magro","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1243283","article-title":"Structure-based design of a fusion glycoprotein vaccine for respiratory syncytial virus","volume":"342","author":"McLellan","year":"2013","journal-title":"Science"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/nature13808","article-title":"Structure and immune recognition of trimeric pre-fusion HIV-1 Env","volume":"514","author":"Pancera","year":"2014","journal-title":"Nature"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1707304114","article-title":"Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen","volume":"114","author":"Pallesen","year":"2017","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)80205-6","article-title":"Core structure of gp41 from the HIV envelope glycoprotein","volume":"89","author":"Chan","year":"1997","journal-title":"Cell"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01202-12","article-title":"Novel, potentially zoonotic paramyxoviruses from the African straw-colored fruit bat Eidolon helvum","volume":"87","author":"Baker","year":"2013","journal-title":"J Virol"},{"key":"B9","doi-asserted-by":"publisher","first-page":"e0007049","DOI":"10.1371\/journal.pntd.0007049","article-title":"Prevalence of Wenzhou virus in small mammals in Yunnan Province, China","volume":"13","author":"Wang","year":"2019","journal-title":"PloS Negl Trop Dis"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.13135","article-title":"Evidence of human infection by a new mammarenavirus endemic to Southeastern Asia","volume":"5","author":"Blasdell","year":"2016","journal-title":"Elife"},{"key":"B11","doi-asserted-by":"publisher","DOI":"10.1101\/2020.05.30.125484","article-title":"Structure-based Design of Prefusion-stabilized SARS-CoV-2 Spikes","volume":"369","author":"Hsieh","year":"2020","journal-title":"bioRxiv"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.2174\/1570163817666200312102137","article-title":"Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein","author":"Sharker","year":"2020","journal-title":"Curr Drug Discov Technol"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(79)90625-4","article-title":"Differences in radiation sensitivity in subpopulations of mammalian multicellular systems","volume":"5","author":"Briganti","year":"1979","journal-title":"Int J Radiat Oncol Biol Phys"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42166","article-title":"New tools for automated high-resolution cryo-EM structure determination in RELION-3","volume":"7","author":"Zivanov","year":"2018","journal-title":"Elife"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.08.008","article-title":"CTFFIND4: Fast and accurate defocus estimation from electron micrographs","volume":"192","author":"Rohou","year":"2015","journal-title":"J Struct Biol"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2024671","article-title":"Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates","volume":"383","author":"Corbett","year":"2020","journal-title":"N Engl J Med"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2607-z","article-title":"Single-shot Ad26 vaccine protects against SARS-CoV-2 in rhesus macaques","author":"Mercado","year":"2020","journal-title":"Nature"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2608-y","article-title":"ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques","author":"van Doremalen","year":"2020","journal-title":"Nature"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507","article-title":"Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation","volume":"367","author":"Wrapp","year":"2020","journal-title":"Science"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd4251","article-title":"Distinct conformational states of SARS-CoV-2 spike protein","volume":"369","author":"Cai","year":"2020","journal-title":"Science"},{"key":"B21","doi-asserted-by":"publisher","first-page":"e1005859","DOI":"10.1371\/journal.ppat.1005859","article-title":"Engineering, Structure and Immunogenicity of the Human Metapneumovirus F Protein in the Postfusion Conformation","volume":"12","author":"Mas","year":"2016","journal-title":"PLoS Pathog"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00842","article-title":"Structure-Based Design of Nipah Virus Vaccines: A Generalizable Approach to Paramyxovirus Immunogen Development","volume":"11","author":"Loomis","year":"2020","journal-title":"Front Immunol"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02039-09","article-title":"Characterization of Lassa virus glycoprotein oligomerization and influence of cholesterol on virus replication","volume":"84","author":"Schlie","year":"2010","journal-title":"J Virol"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.12.009","article-title":"Structural characterization of the glycoprotein GP2 core domain from the CAS virus, a novel arenavirus-like species","volume":"426","author":"Koellhoffer","year":"2014","journal-title":"J Mol Biol"}],"container-title":["Frontiers in Immunology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2020.592370\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T05:23:09Z","timestamp":1604467389000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fimmu.2020.592370\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,4]]},"references-count":24,"alternative-id":["10.3389\/fimmu.2020.592370"],"URL":"https:\/\/doi.org\/10.3389\/fimmu.2020.592370","relation":{},"ISSN":["1664-3224"],"issn-type":[{"value":"1664-3224","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,4]]},"article-number":"592370"}}